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反应抗体

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Results:Monoclonal antibodies IH3,5A12,7E5,7F9 showed specific respo nse to NIH3T3-erbB2 cell and SKBR3 cell,no response to NIH3T3 cell.1H3 also boun d specifically to the extracellular domains of P185 protein that expressed by E.

结果:单克隆抗体1H3、5A12、7E5、7F9可与NIH3T3-erbB2细胞、SKBR3细胞特异性结合,与NIH3T3细胞无反应;1H3与原核表达的c-erbB2胞外区蛋白有特异性反应,而与无关蛋白KDR无反应。

Methods BALB/c mice were immunized by cytoderm antigen of Aspergillus fumigatus, excreting antigen and inactivated conidiospore respectively, to prepare monoclonal antibodies specific to Aspergillus. Cross-reactions between mAbs and antigens from Aspergillus or Monilia genus were characterized by immunofluorescence.

采用烟曲霉细胞壁抗原、分泌抗原和灭活分生孢子,分别免疫BALB/c小鼠,制备特异性的抗曲霉单克隆抗体,免疫荧光法鉴定单克隆抗体与曲霉属和念珠属抗原的交叉反应。

This is due to the fact that AFP in the sample flows through the sample pad and binds the conjugate to form a complex. This complex then migrates down to the membrane and binds to the immobilized anti-AFP antibody A2 of the test line, resulting in the immobilization of the reddish conjugate.

如果样品中含有甲胎蛋白,膜上便会出现肉眼可见的微红色检测线,这是由于样品中的AFP与标记有单克隆抗体A1的免疫金结合形成复合物,复合物向膜上移动与固定在膜上的检测线单克隆抗体A2结合反应,结果在该处形成固定的微红色线条。

This specific preparation methods including: parathion of the initial reaction in acid and zinc metal in the common under redox reaction, a product of the reduction of parathion - parathion, with the sodium nitrite in the cold under diazotization reaction, and bovine serum protein tyrosine on the o-hydroxy combination of the adult immune original and immune rabbit polyclonal antibody preparation.

本发明制备方法具体包括:以对硫磷为反应初始物,在酸和金属锌的共同参与下进行氧化还原反应,得对硫磷还原产物对氨基对硫磷,再与亚硝酸钠在低温下进行重氮化反应后,与牛血清蛋白酪氨酸上羟基的邻位结合,制备成人工免疫原,并免疫新西兰大白兔制备多克隆抗体。

After hybridization of two DNA probes with target DNA, EDTA-〓 and β-diketone come close to each other, and an EDTA-〓-β-diketonate ternary complex having strong and long-lived fluorescence was formed, thus the target DNA was detected sensitively with a detection limit of 6 pM (0.6 fmol per assay) by time-resolved mode.

系统研究了使用EDTA-〓为标记物的石墨炉原子吸收光谱免疫分析法,并将之用于人血清中AFP和水中BSM的测定。该法中使用了EDTA-〓标记SA及生物素标记抗体或抗原,在96孔板中的免疫反应结束后,利用稀硝酸将反应产物分解,然后用石墨炉原子吸收光谱法对免疫反应产物进行测定。

Blood bank type the appliance divided by the blood or blood component in the blood bank 滤 to use amid the leuco - cell, and the aetioporphyrin 品 only be limited by sex usage, that product a time the availability to do away with in order not to leuco - cell, object is the leuco - cell the antibody to spark not the 溶 guts have fever the anteiso -, anaphylaxis, HLA the immune response of of same race isoquinoline ketone body and big and small cytidine taken by leuco - cell k virus k, T lymph cell leukemia the k virus k's infection, prevent hard-pressed synthesis 症 of person respiratory trees

血库型应用于血库滤除血液或血液成分中白细胞用,本品只限于一次性使用,该产品有效去除白细胞,目的是为了避免白细胞抗体引起的非溶血性发热反应、过敏反应、HLA同种异体免疫反应以及由白细胞携带的巨细胞病毒、T淋巴细胞白血病病毒的传染,防止成人呼吸窘迫综合症

Immunohistochemical localization of inhibin α-subunit in normal mature rats was carried out by using Strept Avidin-Biotin-Peroxidase Complex .The results showed that there was positive immunoreactivity of the McAb to inhibinα-subunit in heart ,kidney , spleen , lymph ,adrenal gland and pancreas cells. It indicated that inhibinα-subunit existed in the above tissues , But no positive sites were seen in hypothalamus, pituitary, lung and liver, that means they have no inhibinα-subunit. Compared with the ovariectomized rats, the hypothalamus and pituitary of the rats injected with inhibin-α fragment(1~32)showed an positive reaction, suggesting that inhibin α-subunit could pass through the blood-brain barrier .

用链霉亲和素-生物素-过氧化物酶(Strept Avidin-Biotin-Peroxidase Complex, SABC)免疫组织化学方法,以抑制素α亚基单克隆抗体对正常雌性性成熟大鼠各组织的抑制素α亚基进行定位观察,结果显示:⑴大鼠心脏、肾、脾、淋巴、肾上腺、胰腺均呈阳性反应,表明存在抑制素α亚基;下丘脑、垂体、肝脏和肺组织呈阴性反应,表明其不存在抑制素α亚基;⑵与去卵巢大鼠相比,外源注射抑制素α(1~32)片段的大鼠其下丘脑和垂体出现阳性反应,说明抑制素α亚基能通过血脑屏障。

On the other hand the proliferation activity of ConA-induced splenocytes was enhanced by polysaccharide fraction of Hoshouwu. In mucosal immunity, although polysaccharide fraction of Hoshouwu might not be an effective adjuvant, it enhanced IgM antibody production induced by Pneumococcus vaccine.

何首乌甲醇粗萃物促进自然杀手细胞活性和增强骨髓系造血反应,而何首乌高分子提升全身免疫作用的 T 细胞活性;肠道黏膜系统免疫反应方面,虽然何首乌高分子无法作为有效的佐剂,但可加强肠道病菌感染时的 IgM 抗体反应。

Ternary copolymer ammoniacal latex was obtained using the reaction of nitration and reduction reaction.

通过硝化反应与还原反应在苯乙烯的苯环上接上氨基,利用重氮化反应将登革热抗体与之相交联制成登革病毒胶乳凝集免疫诊断试剂。

Twenty-three virus samples were collected from Siegesbeckia orientalis, Malvastrum coromandelianum, Ageratum conyzoides, Amaranthus spp, Parthenium spp and Artemisia spp, and these samples were tested by TAS-ELISA with monoclonal antibodies against begomoviruses.

从云南赛葵、稀硷、胜红蓟及苋属、银胶菊属和蒿属杂草上采集了23个病毒样本,用双生病毒单克隆抗体进行TAS-ELISA检测发现,赛葵样本VA2、VA7、VA8对SCR18单抗有弱阳性反应;稀硷样本X2对SCR18、106号单抗呈弱阳性反应;其他杂草材料均无明显阳性反应。

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