反应抗体

Western blot results showed that the antibodies recognized proteins with molecular weight of 63000, 59000 and 53000, respectively, the antibodies could specifically react with synthesized peptides of CYP450 enzymes as well as natural human liver microsomal proteins.

Western blot结果显示，三种抗体均可与其合成肽及天然CYP1A2、CYP3A4及CYP4A11蛋白出现特异性反应，识别的相应抗原的相对分子质量分别为63000、59000及53000。

For competitie inhibition, 100-fold molar excess of the unlabelled oligonucleotide was added to the reaction mixture.

为了做超移位分析，抗p50抗体(Santa Cruz Biotechnology， Santa Cruz公司)加入到细胞核提取无物中，在反应前孵化30分钟。

Results :Two weeks after challenge, a remarkable serum antibodyresponse and significant infiltration of numerous polymorphonuclear leukocytes in thelung tissues with higher IL-4、TNF-α and lower IFN-γ production in the lungs were found in theNS placebo treated group compared to the NS control group and SFA treated group.

结果：PA 感染两周后，生理盐水治疗组的肺部炎症反应严重，镜下观肺组织有大量的 PMN 浸润；血清 PA 特异性 IgG 抗体及肺部 IL-4、TNF-α水平显著升高，肺部 IFN-γ水平明显降低。

One pair of primers that amplified the gB gene of pseudorabies viruswas designed and synthesized.PCR technique detecting the DNA of PRV was established after selecting the best reaction conditions.This technique was applied to specifically amplify the 281 bp DNA fragment of the PRV strains including Fa,Fb,Bartha,BJ,GD,V2F4,S,S3,SR,Buk,Shope,Norden,Mink Ⅲ,HB,F8,F9 and F12 in cultured samples.The negative results were achieved from Vero cells,swine vesicular disease virus,hog cholera virus,Japanese encephalitis virus,porcine reproductive and respiratory syndrome virus,porcine parvovirus,foot and mouth disease virus F29 strain,O3I3 strain,T509 strain and O Ⅱ MF249 strain.The results of sequencing showed that the PCR method was of specificity.The sensitivity of PCR reached 15.8 pg of PRV Fa strain DNA.The tissue samples obtained during 1994 and 2000 were detected,and the results showed that the sensitivity of PCR was more sensitive than virus isolation and the Sandwich ELISA.The PCR was applied to detect 191 tissue samples from 31 pig farms obtained from Guangdong,Fujian,Hainan Provinces during 1999 and 2000,50 samples(26.2%)were positive and 22 pig farms(71%)were positive.

根据伪狂犬病病毒gB基因的序列，设计并合成了一对引物，以闽A株细胞培养毒为模板，筛选最佳反应条件，建立了检测PRV的PCR方法应用该方法对Fb、Bartha、BJ、GD、V2F4、S、S3、SR、Buk、Shope、Norden、MinkⅢ、HB、F8、F9、F12等毒株的细胞培养液进行基因扩增，均获得了分子量为 2 81bp的特异性目的DNA片段，而对Vero细胞与FMDV、SVDV、HCV、PRRSV、JEV、PPV等病毒进行检测，结果均为阴性，没有出现交叉反应对PRV毒株扩增的产物测序，结果序列与文献报道一致，证明PCR扩增产物和方法的特异性对 1994～ 2 0 0 0年期间送检的临床样品和保存的PRV毒种，用病毒分离、双抗体夹心ELISA和PCR等 3种方法进行检测，结果前 2种方法检测为阳性的，PCR检测均为阳性；PCR检测为阴性，前 2种方法检测也为阴性；可是，前 2种方法检测为阴性的，PCR却检测出部分阳性；经x2 检验，证明PCR检出率明显高于前 2种方法的检出率对PRV闽A株细胞毒提取物DNA进行检测，其最低检出量为 15 8pg 对 1999～ 2 0 0 0年期间广东、福建、海南等省的 31个大中型猪场送检的 191份病料进行检测。

We did not observe any late rebound of antibodies and there were no humoral rejections.

我们没有看到任何在移植后的抗体复原和体液排斥反应。

D. CBC, AB blood typing and Rh factor, antibody screen, rubella, VDRL/RPR, hepatitis B surface Ag.

D。 全血计数，AB血型及Rh因子，抗体筛查，风疹病毒，性病研究实验室试验/快速血浆反应素环状卡片试验，乙型肝炎表面抗原。

CPLP sensitized with bovine serum albumin were chequerboard titrated by antibody against BSA, and effects of sensitized conditions on latex agglutination test were studied.

结果表明，以0.05 mol／L，pH8.0的Na_2HPO_4-NaH_2PO_4缓冲液稀释CPLP乳液至1.0％，BSA的终浓度为0.5 mg／mL，37℃致敏30 min时，与1∶160稀释的腹水抗体(效价1∶40，000)发生凝集反应的效果最好。

Subsequently Feng N etal thought the levels of theneutralizing antibody response do not always correlate with protection.

随后Feng N et al研究人员认为中和抗体反应的水平并不是总与保护关联。

Our passive immunity patent portfolio concerns the local or topical administration of monoclonal or polyclonal antibody compositions in the prevention and treatment of infectious disease.

通过局部或透皮给送单克隆或多克隆抗体而刺激被动性免疫反应以达到对感染性疾病的预防和治疗。

This thesis mainly focuses on the application of QDs in biology, especially in the cell labeling and immunoassay.

本论文主要工作是量子点在生物学研究中的应用，特别是在细胞标记及抗原抗体免疫反应等方面的生物应用。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。