反应抗体

We optimized reaction system for labelling-antibody in which the optimal amount of the purified anti-Stx2B IgG conjugated with colloidal gold beads was 60μg/mL, the purified antibody for E.coli O157 was 57μg/mL, the optimal pH was 8.2, the size of colloidal gold partical was 20nm, the optimization of stabilizing agent was BSA, the optimization buffer was boracic acid buffer with pH 8.2, the optimization of preserving fluid and eluant was boracic acid buffer with pH 8.2 including 5mM NaCl-1%BSA, confining liquid for NC membrane was 0.01% PBST with 3%BSA, the amount of polyclonal antibody against E.coli O157 and Stx2 conjugated with colloidal gold beads for conjugate pad was 3μg respectively, the amount of anti-Stx monoclonal antibody for test line was 0.1μg and 1μg for E.coli O157, the amount of goat anti rabbit IgG for control line of both GICA were 1μg.

测定了胶体金颗粒的最优化反应体系：胶体金颗粒的大小为20nm；抗体与胶体金溶液结合的最佳pH约为8.2；最佳蛋白结合量分别为抗大肠杆菌O157 IgG为57μg/mL，抗重组Stx2B IgG为60μg/mL；最佳稳定剂为BSA；最佳缓冲液为pH8.2硼酸溶液；最佳金标保存液和洗涤液为5mM NaCl-1%BSA的pH 8.2的硼酸缓冲液；NC膜的封闭液为3%BSA的0.01mol/L PBST；Stx2试纸条和大肠杆菌O157试纸条的质控线上的羊抗兔IgG的多克隆抗体最佳点样量均为1μg，其检测线的抗Stx2的单克隆抗体的点样量为0.1μg、抗大肠杆菌O157的单克隆抗体的点样量为1μg，结合垫上的金标抗大肠杆菌O157和重组Stx2B多克隆抗体点样量均为3μg。

The limited detectable concentration was 85μg/L. The cross-reaction rate between PAb and other pyrethroid pesticides was low, except deltamethrin.

以抗体与甲氰菊酯的反应率为100%，抗体与其他4种菊酯类农药交叉反应率很低，与溴氰菊酯交叉反应较高。

Distinction instillment different concentration antigen.

分别滴加不同浓度的抗原使其发生反应，测量傅里叶变换红外谱和加入抗体前后反射谱的变化，结果表明硅烷化效果明显，生物抗原较好的固定，抗原抗体反应前后反射谱有明显的红移，且红移量于滴加抗原浓度增加而增加，并且呈现出良好的非线性关系。

In order to discuss the interaction of viremia and antibody caused by reticuloendotheliosis and avian leucosis virus of subgroup J, REV, ALV-J and REV+ALV-J were injected to SPF chicken at 2 d separately, then tested the viremia and antibody dynamically for 7 weeks. The results indicated that compared with single infection, the dynamic viremia level of REV and ALV-J wasn't obviously different when coinfected.

为了研究禽网状内皮增生病病毒、禽J亚群白血病病毒共感染时对彼此病毒血症及抗体反应的影响，2日龄SPF鸡分别接种REV、ALV-J或同时2种病毒造成共感染，对各感染组7周内的病毒血症及抗体反应进行动态检测。

There is also in the skin lesions of psoriasis immune globulin, complement, anti-IsA factor, anti-keratin antibodies and parakeratotic nuclei antibody, etc., these factors result in abnormal T lymphocytes in the immune cycle of separatist injury and the number of T lymphocytes in significantly reduced, such as the Con A stimulus-response is reduced, while phytohemagglutinin and pokeweed mitogen factor is a normal reaction, this phenomenon is to support T-cell subsets flawed.

在牛皮癣的皮损中还有免疫球蛋白、补体、抗IsA因子、抗角蛋白抗体及角化不全细胞核中有抗体等，这些免疫异常因子造成T淋巴细胞的周期分裂性损伤和T淋巴细胞数量明显减少，如对刀豆球蛋白的刺激反应性下降，而对植物血凝素和商陆有丝分裂因子却反应正常，这种现象支持T细胞亚群存在缺陷。

To develop standard WNV diagnostic tools, four monoclonal antibodies against WNV envelope protein domain III were produced. The isotype, the reactivity with denatured and native antigens, the affinity and specificity of antibodies have been characterized. Two MAbs (4B3 and 2F5) were used to establish an antigen capture-ELISA detection system.

为了建立有效的WNV检测方法，本研究制备了四株抗WNV囊膜蛋白第三结构域的单克隆抗体，鉴定了抗体的亚型、抗体和变性及非变性抗原结合的强度、抗原和抗体结合的亲和力常数，进行了免疫荧光分析、测定了抗原表位竞争、抗体的中和能力等，并检测了上述单抗与日本乙型脑炎病毒的交叉反应情况。

Results The level of antibodies against monocytes (34.94%) were higher than that of the antibodies against lymphocytes (26.67%) in pretransplant patients. The level of antibodies in first time transplant patients (32.5%) was lower than that of antibodies in retransplant patients (59.7%). The patients with intensity of antibodies against monocytes PRA more than ≥50% only accounted for 1.87%, 10%＜ MPRA ＜50% for 17.62% and MPRA ＜10% 15.44%,respectively.

结果 肾移植术前患者的抗单核细胞抗体水平较抗淋巴细胞抗体水平高，其百分比分别为34.94%和26.67%；而从患者初次和再次肾移植前的比较中可见，初次比再次产生的抗单核细胞抗体水平要低，其百分比分别为32.05%和59.7%；对于抗单核细胞抗体群体反应性抗体的强度，MPRA≥50%的只占1.87%，而50%＜MPRA＞10%以及MPRA＜10%的分别占17.62%和15.44%。

The new method formulizes the immune response as a quaternion AIR=, where G denotes exterior stimulus or antigen, I denotes the set of valid antibodies, R denotes the set of reaction rules describing the interactions between antibodies, and A denotes the dynamical algorithm describing how the reaction rules are applied to antibody population.

新方法将免疫响应过程建模为一个四元组 AIR=，其中 G 为引发免疫响应的外界刺激，即抗原； I 为所有可能抗体的集合； R 为抗体间相互作用的规则集合； A 为支配抗体反应、指导抗体进化的动态算法。

The results showed that antibody response were observed in 19 (27.1%) of the 70 children with pneumonia. Excluding one case of cross-reaction, the others 18 cases, were diagnosed as Hib infection five caese had no Hib antigen evidence. Their diagnosis were confirmed only by the antibody response.

结果有19/70(27.1%例)肺炎患儿产生Hib OMP抗体反应，除外1例交叉反应，余18例诊断为Hib感染，其中5例未查到Hib抗原，以Hib OMP特异抗体反应得到诊断。

It is to use a high degree of specificity of the antibody immune response and chromatography analysis technology, marked by colloidal gold β-HCG and mouse monoclonal antibody IgG and fixed in the nitro-cellulose membrane of the anti-α-HCG and sheep anti-mouse antibody IgG Antibody, the specificity of detection in urine HCG.

是利用高度特异性的抗原抗体反应及免疫层析分析技术，通过胶体金标记的β-HCG单克隆抗体和鼠IgG以及固定于硝基纤维素膜上的抗α-HCG抗体及羊抗鼠IgG抗体，特异性的检测尿液中HCG。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。