反应抗体

The level of PGE was assayed by spectrophotometer; Meanwhile, 3H-TdR incorporation method was used to detect ConA- and LPS- induced splenocytes proliferation and the production of interleukin-1 and IL-2. The content of antibodies to CII was determined by ELISA method. Pathological examination of articulus tissue was observed by Hematoxylin-eosin stain.

检测小鼠足爪肿胀度并对炎症进行评分；测定胸腺指数和脾脏指数；~3H-TdR参入法检测胸腺、脾淋巴细胞增殖反应；~3H-TdR参入法测定白细胞介素-1(interleukin-1，IL-1)和IL-2水平；ELISA法测定小鼠血清中抗CⅡ抗体含量；分光光度法检测足爪局部产生的前列腺素水平；HE染色法对关节组织作病理检查。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

The bacterially expressed GP5 protein was recognized by specific antibody,confirming that the protein had biological activity.

免疫印迹试验证实，表达的融合蛋白能与特异性血清抗体反应，表明表达的PRRSV GP5蛋白具有良好的生物学活性。

Furthermore, we take the bicolor (i.e. green and orange) CdTe QDs as the effective pH-sensitive fluorescent probes for dual simultaneous and independent detection of viruses.

我们先采用单色量子标记的F0F1-ATP脂酶体作为病毒检测器，利用抗体抗原反应，实现了H9型禽流感病毒的快速、灵敏检测。

Methods The antibody of Borrelia burgdoferi in the crowd serum was detected by immunofluorescence, and the spirochete DNA was tested with PCR.

人群血清伯氏疏螺旋体抗体的检测采用免疫荧光法，以聚合酶链反应检测伯氏疏螺旋体DNA。

Any pathogen which targets and destroys bursal cells will have a devastating effect on antibody-dependent immune responses.

任何针对并破坏法氏囊细胞的病原将对抗体依赖性免疫反应造成毁灭性的影响。

The inflammation reaction hyperplactic stage and lymphatic nodular stage are of nonspecific morphological changes and should be diagnosed with the help of positive tuberculin test and obvious increase of adenosine deaninase ; tuberculous nodular stage has a great number of epithelioid cells,and Langhans cells, caseous necrosis stage is characterized by a great deal of necrotic tissue and debris,a small number of fragmented epithelioid cells

炎性增殖反应期非特异性形态学变化，需要结合结核抗体阳性和腺苷酸脱氨酶明显增高有助于诊断，结核结节期主要可有较多类上皮样细胞及郎罕氏细胞；而干酪样脓样坏死主要见大量坏死组织及碎屑、少数残碎不全类上皮样细胞，此期主要能查到抗酸菌为特点；纤维增殖期，抽出物难取，仅见少数纤维组织、纤维细胞和黏液间质为其特征，提示结核恢复、瘢痕形成所致。

These antibodies were first found to react with cerebellar cells.

这些抗体被首次发现的反应与小脑细胞。

Initially this method was employed to dected antibodies in sera from 50 clonorchiasis patients; 46 sera were found to be positive with a positivity rate of 92%.

初次探索方法时曾检测50份华支睾吸虫病人血清，46份阳性反应，阳性率92％，间接萤光抗体阳性率为74％。

We use the kidney to research the issue of that cystamine can regulate kidney to improve the disease or not. In flow cytometry, we use CD8/CD4/CD25 makers to observe the balance of TH1/TH2, and we find that cystamine can raise TH2 expression to produce autoantibodies and also can induce overexpression of Treg to ease inflammation.

为了同时在免疫方面观察胱胺是否也有著能减缓SLE的倾向，进而深入了解观察肾脏这个重要的次级免疫器官，探讨cystamine是否对於肾脏也具有重要的调节作用进而能改善病症，在本实验中以flow cytometry对免疫倾向的确认，透过CD8/CD4/CD25 maker观察TH1/TH2 balance，发现胱胺能有效的反转SLE大量倾向TH2进而产生的自体抗体，同时也能增加 Treg的高量表达进而去抑制免疫反应缓解发炎。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。