反应抗体

Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%,0 and 38% respectively in sorted GPA positive cells of 3 samples by FISH,and the results of GPA+ cells of the 3 cases by PCR were positive,negative and positive respectively,which were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16～24周的孕妇血中分离出NRBCs，并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果，3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%；其PCR结果，分别为阳性、阴性、阳性，并在引产后得到性别证实。

Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%0 and 38% respectively in sorted GPA positive cells of 3 samples by FISHand the results of GPA+ cells of the 3 cases by PCR were positivenegative and positive respectivelywhich were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16～24周的孕妇血中分离出NRBCs，并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果，3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%；其PCR结果，分别为阳性、阴性、阳性，并在引产后得到性别证明。

Trimolecular complex will be activated T cells, immune cells leading to a variety of abnormalities, a variety of cytokines and antibody, and then, like a domino effect caused a series of the same immune response, resulting in blood vessels and synovial inflammation .

三分子复合物会激活T细胞，导致多种免疫细胞的异常，产生各种不同的细胞因子和抗体，然后会像多米诺骨牌效应一样引起一系列的免疫反应，从而导致血管和关节滑膜的炎症。

Histology, immunohistochemistry and electron microscope were used to study the structures and functions of the endostyle of amphioxus and the thyroid gland of grey mullet.

免疫组织化学结果揭示，内柱第3和第5区带细胞的顶端胞质及纤毛对鼠抗人甲状腺球蛋白抗体呈免疫阳性反应。

In this paper, we built and discussed a virus dynamics model with antibody immune response.

建立并讨论了一类具有抗体免疫反应的病毒动力学模型。

The glycyrrhizic acid antigen is prepared through the process including following steps: dissolving glycyrrhizic acid in N, N-dimethyl formamide, dioxane or dimethyl sulfoxide, heliophobically adding N-hydroxy succinimide, reacting with dicyclohexylcarbodiimide at 0-10 deg.c for 2-5 hr; and reacting with carrier protein at normal temperature for 4-6 hr to obtain glycyrrhizic acid antigen.

本发明所提供的甘草酸抗体，是用甘草酸抗原免疫动物，然后从被免疫动物的血清中分离得到的；所述甘草酸抗原按如下过程制备：先将甘草酸溶解在N，N-二甲基甲酰胺、二氧六环或二甲基亚砜中，避光加入N-羟基琥珀酰亚胺，然后在0-10℃条件下与二环己基碳二亚胺反应2-5小时；接着与载体蛋白常温反应4-6小时，得到甘草酸抗原。

The reacting tite with NDPK-A was 1:800 and theantisera was specific.Conclusions: The purified rhNDPK-A protein exists mainly in the form of hexamer;rhNDPK-A protein can promote the survival of spleen cell and may take part in theadjustment of cell growth by the way of changing cell cycle.

ELISA法检测所制备的抗体的反应效价为：1：800，western blotting试验结果证实，该抗血清具备较好的特异性，能与rhNDPK-A蛋白发生反应，结论：rhNDPK-A蛋白可能主要以六聚体形式存在，直接观察的蛋白结构与文献报道的X衍射结果相似；rhNDPK-A蛋白对正常小鼠的脾细胞的存活有促进作用，推测很可能以信号转导的方式调控细胞周期从而达到对细胞生长的调节作用。

Increasing of the proliferation and differentiation of lymphocytes in LP were found. Conclusions Lymphocyts in LP play an important role on the mucosal immunity of SPF mice when they are first inoculated with Candidas. The reducing amount of Candidas in intestine is related to increasing of the specific IgA antibodies in the intestinal mucus.

带菌动物首次白念菌肠内给入后以固有层中淋巴细胞反应为主，增殖及分化均增加；白念菌总量和粘附数量减少与局部粘膜免疫反应及特异IgA抗体含量上升的关系十分密切。

Moreover, in order to make clear whether IV infection touches off the cause and its pathogenesis of artherosclerosis inflammatory reaction. The study also uses IV of type A1 and A3 to infect repeatedly Jimpy mice with E gene defect, to observe AS histology change of Jimpy mice aorta and detect the change of IV antibody and inflammatory reaction medium (eg.TNF-α, IL-1) in Jimpy mice serum and express of monocyte chemotactic factor( MCF-1) and nuclear factor kB in Jimpy mice aorta tissue .

另外，为了弄清流感病毒感染是否是触发动脉粥样硬化炎症反应的发生原因及其机理，本研究还用A1、A3型流感病毒反复感染载脂蛋白E基因缺陷小鼠，观察小鼠主动脉的动脉粥样硬化组织学改变，并检测小鼠血清中流感病毒抗体、炎症反应介质（如TNF-a、IL-1等）变化情况以及小鼠主动脉组织中的单核细胞趋化因子-1（MCP-1）和核转录因子kB表达情况。

The positive seroconversion rates of neutralizing antibody were 100%(n=27 GMT 2.89 IU/ml) and 100%(n=30, GMT 5.29 IU/ml) respectively by pre-exposure and post-exposure schedules.

结果 全部受试者仅显示12%的轻度副反应，无中强度副反应；不管以暴露前或暴露后方案接种，全程注射后的中和抗体阳转率均为100%。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。