反应性地

After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象，提取细菌基因组DNA，用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切，回收大小为1～5Kb的DNA片段，将其连接入原核表达载体pET-28a-c，最后转化宿主菌，结果成功地构建了基因表达文库，为后续的基因筛选工作奠定基础；另外，本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计，以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板，进行PCR扩增反应，结果表明成功地获得两个新基因：23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸)，并进一步做了不同物种之间的分子系统发育分析，构建了基于23S rRNA基因的邻接法系统发育树，阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

Studing on samarium diiodide promoted asymmetric intramolecular reducing coupling reactions, we have made the resolution of the racemic biaryl dials by using enantiomeric N-tert-butanesulfinamine as the resolving reagent successfully and thus obtained a series of axially chiral biaryl dials with high enantiomeric excess.

中文摘要在二碘化钐促进的分子内不对称还原偶联反应中，我们不仅对联芳基二醛底物的合成路线进行了优化，还发现N-叔丁基亚磺酰胺辅基可以作为手性试剂，成功地对联芳基骨架二醛化合物进行拆分，得到高对映体过量的轴手性二醛类化合物。

Good thermal stability and self-extinguishing behavior was observed with the cured polybenzoxazine resins. Second, furan-containing benzoxazine monomers, P-FBz, BPA-FBz, and 4FP-FBz were prepared using furfurylamine as a raw material. Formation of furfurylamine Mannich bridge networks in the polymerizations of P-FBz and BPA-FBz increased the cross-linking densities and thermal stability of the resulting polybenzoxazines. P-FBz and BPA-FBz- based polymers also exhibited high glass transition temperatures above 300℃, high char yields, and low flammability with a limited oxygen index value of 31. The dielectric (Dk3.21–3.39) and mechanical properties (high storage modulus of 3.0–3.9 GPa and low coefficient of thermal expansion of 37.7– 45.4 ppm) of the P-FBz and BPA-FBz-based polymers were superior or comparable to other polybenzoxazines. 4FP-FBz used for polybenzo- xazine modification by means of formation of benzoxazine hybridation with P-FBz.

第二部份以2-呋喃甲胺为起始物，制备了P-FBz、4FP-FBz与BPA-FBz 三个含呋喃官能基之氧代氮代苯并环己烷的单体；BPA-FBz与P-FBz於开环聚合中形成之Mannich bridge网状结构有效地提升聚氧代氮代苯并环己烷聚合物之交联密度与热安定性；Poly与Poly具有大於300℃之玻璃转移温度、高的焦炭生成率与具有31之极限氧指数(Limited oxygen index， LOI)的难燃性特质，Poly与Poly之介电常数(Dk=3.21~3.39)与机械性质[3.0~3.9 GPa的储存模数与37.7~45.4ppm之低热膨胀系数( Low coefficient of thermal expansion， Low CTE)]均较其他聚氧代氮代苯并环己烷为优；4FP-FBz具有低之介电常数(Dk=2.7)，以其作为降低介电常数之反应型改质剂，与P-FBz混成，证实可有效地降低Poly的介电常数。

Using a rat model of chronic constriction injury of the sciatic nerve, we found that pharmacologic ETA receptor antagonism acutely and significantly reduced thermal and mechanical hyperalgesic responses 5 days after injury.

利用小鼠坐骨神经慢性收缩性损害模型，我们发现， ETA 受体拮抗药能快速地明显地降低损伤5天后热和机械的过敏性痛觉反应。

A one-pot chemoselective reduction and lactonization of(4S,5R)-hemiesters was carried out to afford high-yielding(3aS,6aR)-lactone,a key chiral building block for the biotin synthesis. One-step introduction of carboxybutyl chain onto(3aS,6aR)-thiolactone was reached upon treatment of zinc reagent 25 in the presence of nanopalladium via an improved Fukuyama coupling under mild reaction conditions.

在侧链引入过程中，采用纳米钯LDH-Pd~0为催化剂以改进的Fukuyama偶联反应一步高效地引入-生物素五碳羧酸侧链，紧接着创造性地以离子氢化的方式建立了-生物素的第三个手性中心。

Multiplex-PCR detection method for Pasteurella multocida and Haemophilus parasuis had been developed based on published single PCR for these pathogens. The PCR reagents, annealing temperature and the PCR procedure were optimized and the primers were screened, the PCR products including 457bp of PM and 1090bp of HPS were obtained just by one test.

在已经建立的多杀性巴氏杆菌、副猪嗜血杆菌的单项PCR诊断方法的基础上，通过对扩增条件和引物的筛选，成功地建立了PM、HPS复合PCR诊断方法，利用一次PCR反应，即可同时扩增多杀性巴氏杆菌的457 bp和副猪嗜血杆菌的1090 bp的特异性片段。

I am not very happy acting pleased whenever prominent scientists overmanify intellectualellightenment,stoutheartedly outvociferating ultrareactionary retrogressionists ,characterisically unsupernaturalizing transubstantiantively,philosophicoreligious,incomprehensiblenesses, anthtopomorphologically

有些杰出的科学家，有时过分夸大智力超常开发的意义，我对此深表不以为然，因为那些勇敢地站出来反对超常反应的非超常人士明明白白地从人类的生物多态性的角度指出，他们所说的哲学信仰是难以理解，但并非无法理解。

Excitation functions of neutron, proton and α particle emission prior to scission measured in the reactions 28 Si+ 164,167,170 Er→ 192,195,198 Pb are studied using an extensive fission diffusion model and it is found that the model can well describe the trend of particle multiplicities of Pb isotopes measured experimentally as a function of the bombarding energy,which indicates the existence of isotope effects in prescission particle emission.

用推广的裂变扩散模型研究了2 8Si+16 4，16 7，170 Er→192 ，195，198Pb反应中测量的断前中子、质子和α粒子多重性的激发函数。发现扩散模型能很好地拟合这些轻粒子多重性随裂变系统激发能和Pb同位素的变化趋势。表明断前粒子发射存在一个同位素效应。进而在一个考虑了裂变过程中摩擦效应的统计模型的框架内，在In和Er这两个元素的同位素链中，考察了同位素效应与裂变碎片质量不对称性和裂变系统尺度的关系。计算结果表明：同位素效应的存在不依赖于裂变系统的大小和不对称性。此外，当裂变系统具有高的中质比时，轻带电粒子发射将不再敏感于裂变延迟时间的长短，而中子发射则非常敏感。

Oil and polyol firstly hydrolyze, and then react with maleic anhydride to give semi ester, the residual maleic anhydride hydrolyzing to give maleic acid, finally are adducted with DCPD. The result of experiment shows that the synthesized resin is of perfect air drying performance and improved flexibility and anti-greening because of the use of oil, meetin...

试验结果表明，油与多元醇在210～220℃下先醇解，然后与顺酐反应形成半酯，剩余顺酐水解成顺丁烯二酸，然后与DCPD加成，合成的树脂具有优良的空干性；同时油的使用极大地提高了涂膜的柔韧性和抗绿化性，得到的树脂性能上可满足涂料用不饱和聚酯树脂的要求。

By defining several localindices which can be easily calculated from these ground-state wavefunctions, we first succeeded in explaining bond lengths, orientation ofelectrophilic aromatic substitution, and local aromaticity of these benzenoidhydrocarbons within the VB theory. Moreover, the energy splittings betweenthe ground state and first excited state are applied to correlate with theiroverall aromatic characters, the results are also in good agreement withrelated predictions from MO theory.

通过定义一些可由基态波函数直接计算的局域指标，首次在VB模型下成功地解释了苯型烃的键长、亲电取代反应的方向及局域芳香性，并用基态与第一激发态的能量差讨论了这些分子的整体芳香性，得到了与分子轨道理论的预测基本平行的结果。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。