反应学

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

SLE is involved in immediate allergic II~IV types reactions. The overexpression of immediate allergic reactions can be the potential factor to induce cell apoptosis. In the past researches, cystamine which is the inhibitor of TG2 and caspase can slow down the expression of SLE through suppressing cell apoptosis and also can prolong the ratio of surviving in NZB/W F1 mice model in serological and protein test.

其广泛的牵涉了II~IV型的过敏反应，过敏反应的过分激活为自体抗体大量产生引发细胞apoptosis的潜在因素，在本实验室过去曾发现在NZB/W F1 mice作为实验动物模式之下，以cystamine此TG2及caspase的抑制剂具有在血清学及蛋白检测中均可透过抑制细胞的凋亡，进而减缓SLE达到保护的作用，改善狼疮病症，且延长其存活率。

"Palmar-plantar erythrodysesthesia typically presents with dysesthesia and tingling in the hands and feet, which usually appear 2 to 12 days after administration of chemotherapy," note the authors led by Domenica Lorusso, MD, from the department of oncology at Catholic University of the Sacred Heart, in Campobasso, Italy.

手掌脚掌红肿感觉异常，也称手足症候群或手至足症候群，是不舒服且相对常见的皮肤毒性反应，通常是对某些化学治疗药物；目对症候群仍所知不，为了厘清这个问题，专家于7月号癌症学志发表了一篇综论性文章，讨论如何处理这个不幸的不良反应

The experiments in the herbicidal activity inhibiting bacteria activity and promoting animal growth activity of these novel compounds are also first studied.

分析结果显示两个反应体系中分别含有16种和17种成分。依据有关反应理论和质谱等波谱学理论，解析了33种成分的结构。

Radiologically, it is characterized by close approximation and contact of adjacent spinous processes, resultant enlargement, flattening and reactive sclerosis of apposing interspinous surfaces.

放射学表现为邻近的棘突极度接近，反应性肥大，并列的棘突间表面变扁及反应性硬化。

Results AHS was an acute severe drug reaction with characteristic symptoms, including generalized skin eruption, fever, lymphadenectasis, eosinophilia, and multiple visceral involvement.

结果 AHS是一种急性严重药物反应、临床表现为广泛皮损，伴发热、淋巴结肿大、多脏器受累、嗜酸粒细胞增多等血液学异常为特征的严重药物反应。

Objective To study the statement of immunoreaction of c Jun and functional recovery of spinal cord injury in rats after fetal spinal cord transplantation.Methods Experimental rats were divided into two groups:spinal cord hemisection injury combination with transplants of FSC to injured site group,and spinal cord injury that received gelfoam pledgets group.On the lst,3th,7th,14th and 28th day after operation,6 animals in each group at each time phase,were observed for their function recovery with the methods of praxiology and electro physiological examination,the animals were then sacrificed for the observation of the statement of c Jun reaction by immnochemistry method and computer image analysis system was used for quantitative analysis.

目的：研究大鼠胚胎脊髓移植后能否影响，c-Hun免疫反应的表达和脊髓损伤后大鼠功能的恢复，方法；将动物分为脊髓半切洞损伤加胚胎脊髓移植组和单纯脊髓板切洞损伤加明胶海绵填塞组，每个时相点每组6只动物，术只1，3，7，14和28天，应用行为学和电生理检查观察大鼠功能恢复情况，应用免疫细胞化学方法，c-Jun免疫反应的表达，采用计算机图像分析技术，进行定量分析。

The aim is to use rat model of formalin result to pain to observe rat praxiology responses after giving ASA , The results found that: The rats showed spontaneous pain-related responses after formalin test. After being injected formalin into one hindpaw, the rats of F group immediately showed intense pain-related response: flinching / shaking and licking / biting injected paws.

在福尔马林炎性痛动物模型测定给药后大鼠的痛行为反应，发现ASA可以明显抑制F引起的双相痛反应，故从行为学的水平确定了ASA有显著的镇痛效应。

To monitor the effieciency of this site-directed serodiagnostic procedure, the synthetic peptides adhered to the microplates was biotinylated in situ with biotin of an extended spacer arm and avidin-biotinylated horseradish peroxidase complex was used.

为控制定位血清学检测质量，本研究应用长臂生物素对吸附于反应板上的合成肽作&原位&标记，经ABC结合和底物显色并计算吸附指数，首次为检测合成肽对反应板吸附性建立了一种简单可靠的方法。

Injection of BTX-A intraperitoneally significantly reduces acetic acid-induced nociceptive behaviors after 1 week and 4 weeks, decreases the level of serumal TNF.

腹腔注射BTX-A能缓解乙酸所致的大鼠内脏损伤的疼痛行为学反应，减少血清TNF释放，BTX-A可能通过减少血清TNF释放，减轻炎性反应，减少疼痛刺激。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。