反应作用

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

The proceed also can be used analogically this simulation model to simulate a series simulation, to erect the performance or operation diagram of reaction temperature v.

此外，於实务上因反应腔化学作用、工件材料、抽气速度等操作条件、生产批次之不同，通过冷却管排之工作介质的设定条件亦需随之变化，此亦可经由类似本模拟模型进行一系列之模拟，并据此建立各装置之反应温度－冷却剂通过水量等操作或性能线图，以提供使用端或操作人员於操作时之设定依据，此亦是本研究探讨的目的。

In this paper, based on the analysis of the effects of the structure of UASB reactors and the processes of reactions on corrosion, the corrosion mechanism is discussed, and three measures to prevent the corrosion of the UASB reactor are presented, I.e., improvement of the anticor rosion design, application of anticorrosion materials and preservative treatment of the equipment.

由于上流式厌氧污泥床UASB内发生的反应主要属于化学过程和生物过程，因此UASB反应器内壁极易发生腐蚀，从而影响反应效果，降低设备使用寿命，增加维护费用在分析了上流式厌氧污泥床反应器的结构与工艺对腐蚀的作用的基础上，讨论了腐蚀机理，提出了应在改进防腐设计、采用耐腐蚀材料和对设备进行防腐处理等3个方面采取措施，从而防止UASB反应器内的腐蚀现象

Aside from pancreatic enzymes activiation and autodigestive process, recent investigations have established that the upregulation of inflammatory mediator are believed to be the critical steps in the progression of mild pancreatitis to severe pancreatitis. Tumor necrosis factor-, ThyomboxaneA2 ,ProstaglandinsI2 are all important inflammatory mediator. TNF-( is one of cytokines. It is important to the over-production of ICAM-1,VCAM-1, TXA2 andPGI2. The ICAM-1 and VCAM-1 mediate both leukocytes adhesion and migration through the endothelium into tissues to connect with injuried target cells. The TXA2 and PGI2 are the production of arachidonic acid. They are responsible for the tissue ischaemia.

肿瘤坏死因子（Tumor necrosis factor-， TNF-、血栓素A2（ThyomboxaneA2，TXA2），前列环素（ProstaglandinsI2，PGI2）均是炎症反应中重要的炎性介质，其中肿瘤坏死因子-是非常重要的细胞因子之一，它由活化的单核细胞产生，在细胞间黏附分子（Intercellular adhesion molecule-1，ICAM-1）及血管细胞黏附分子(Vascular cell adhesion molecule-1，VCAM-1的表达、血栓素A2及前列环素过量生成方面起着重要的作用，前两者是介导白细胞与内皮细胞黏附，迁移于组织并与受损的靶细胞相结合的主要媒介，是炎症反应中的重要介质，后两者是花生四烯酸的代谢产物，亦是重要的炎性介质，参与构成血液循环障碍。

Conversely, in negative reinforcement, the frequency of a response increases because it is followed by the removal of an aversive stimulus.

作用下，反应频率由于反应发生后令人厌恶的刺激消除而有所增加。

The system exhibits favorable catalytic activity for the oxidation of benzylic and allylic alcohols, in spite of low activity observed for the oxidation of heterocyclic and aliphatic alcohols.

探讨了TEMPO-IL/CuCl催化体系的催化机理，铜离子是反应的活性中心。针对 TEMPO/CuCl 催化体系反应速度较慢的问题，研究了碱对 TEMPO/CuCl 体系的促进作用。

Therelative rates of the Rh-mediated carbenoid insertion into the benzylic C - H bonds of aseries of para-substituted phenyl substrates: methyl 2-diazo-3-oxo-6-(4-substituted phenyl)-4-propylhexanoate 47a - g, have been measured by an indirect intramolecular competitionmethod under the condition in which the possible steric and conformational effects have beenminimized.

为了将立体因素和构象因素最大程度减小，以便较为准确地评价电子效应对该反应的影响，设计并合成了不同对位取代基的化合物4-丙基-6-(4-取代苯基)-2-重氮基-3-氧代己酸甲酯47a-g，并研究了它们在不同Rh催化剂作用下的分子内C-H插入反应。

In this thesis, the three series of P, O and P, N bidentate ligands chelated palladium complexes ￡40-46} were designed and synthesized for Suziki cross-coupling reaction.

研究了它们对Suzuki偶联反应的催化作用，优化了这些催化剂在联苯类化合物合成中的反应条件。

Bornyl oxalate was formed smoothly by esterification of β-pinene with anhydrous oxalic acid using solid superacid as catalyst. Borneol was obtained by saponification of bornyl oxalate with NaOH solution.

在固体超强酸催化作用下β-蒎烯和无水草酸平稳地发生酯化反应，生成草酸龙脑酯；加入氢氧化钠溶液发生皂化反应得到龙脑。

Effects of vapour of organic solvents on the poly- was studied by the differential scanning calorimetry.

用差示扫描量热研究了几种有机溶剂蒸气对聚对苯二甲酸乙二酯的作用，表明可分为反应性的和非反应性的两种。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。