反序列

Secondly, according to the theory of retrieving model using time series and the self-memorization principle, the grey self-memory model based on grey system theory and phase space self-memory model established using chaotic theory are put forward, and the two kinds of models are applied to the analogy and forecasting of hydrologic time series, and the application field of the self-memory model is further expanded from extent and profundity.

更进一步，根据时间序列反演建模基本理论和系统自记忆性基本原理，提出了基于灰色系统理论的灰色自记忆预报模型和基于混沌理论的相空间自记忆预报模型，并应用于水文时间序列的模拟与预测，从广度和深度上更加拓广了自记忆模型的应用范围。

Lastly, based on analysis on phylogentic relationships among 39 ciliates combined with their main morphologic characters, some conclusion were drawn: 1 the monophyly of the peritrichs was strongly supported, and its subclass rank in the class Oligohymenophorea is reasonable; 2 the genus Epistylis might not be monophyletic, and the peristomial area, especially the peristomial lip, might be an important phylogenetic character within the genus Epistylis; 3 the taxonomic rank of the genus Carchesium and the genus Campanella were suggested to be adjusted; 4 Opisthonecta probably diverged from peritrichs with stalks; 5 the phylogenetic tree of peritrichs was reconstructed based on the molecular information inferred from SSrRNA gene sequences and the morphology.

测定了6科8属13种缘毛类纤毛虫的SSrDNA序列，将构建的最大简约树和邻接树与形态学相结合，分析得出：1）缘毛类纤毛虫是单系的，其分类地位是隶属于纤毛门寡膜纲缘毛亚纲：2）指出累枝虫属可能不是单系的，口围区是累枝虫属中重要的系统发育特征；3）建议调整独缩虫和聚钟虫属的分类地位；4）得出后游虫可能是由有柄缘毛类进化而来的类群；5）基于缘毛类反口区结构和SSrRNA基因序列提供的信息重建了缘毛类纤毛虫的系统发育树。

Objective:To design the antisense RNA gene and hammerhead ribozyme gene according to the hTR sequence in the intrahepatic bile duct cancer cell,and insert them into the eukaryon expression vector pTriEx-4 separately.

目的：用反转录PCR法，调取肝内胆管癌细胞内的端粒酶RNA基因，并针对其序列设计反义RNA封闭基因及锤头状核酶切割基因序列，并将其构建入真核表达载体pTriEx-4内。

This article makes use of the chaotic power spectrum de-convolution, adder, translational, zoom to construct a new pseudo-random sequence. Through theoretical analysis and simulation experiments, the characteristics of the sequence are able to meet Golomb's three public supposals.

本文就是将混沌连续的功率谱进行反褶、平移、相加、缩放等变换构造出了一种新的伪随机序列，通过理论分析和仿真试验，初步验证了该序列的各项特性是满足了Golomb的3个公设。

One pair of primers that amplified the gB gene of pseudorabies viruswas designed and synthesized.PCR technique detecting the DNA of PRV was established after selecting the best reaction conditions.This technique was applied to specifically amplify the 281 bp DNA fragment of the PRV strains including Fa,Fb,Bartha,BJ,GD,V2F4,S,S3,SR,Buk,Shope,Norden,Mink Ⅲ,HB,F8,F9 and F12 in cultured samples.The negative results were achieved from Vero cells,swine vesicular disease virus,hog cholera virus,Japanese encephalitis virus,porcine reproductive and respiratory syndrome virus,porcine parvovirus,foot and mouth disease virus F29 strain,O3I3 strain,T509 strain and O Ⅱ MF249 strain.The results of sequencing showed that the PCR method was of specificity.The sensitivity of PCR reached 15.8 pg of PRV Fa strain DNA.The tissue samples obtained during 1994 and 2000 were detected,and the results showed that the sensitivity of PCR was more sensitive than virus isolation and the Sandwich ELISA.The PCR was applied to detect 191 tissue samples from 31 pig farms obtained from Guangdong,Fujian,Hainan Provinces during 1999 and 2000,50 samples(26.2%)were positive and 22 pig farms(71%)were positive.

根据伪狂犬病病毒gB基因的序列，设计并合成了一对引物，以闽A株细胞培养毒为模板，筛选最佳反应条件，建立了检测PRV的PCR方法应用该方法对Fb、Bartha、BJ、GD、V2F4、S、S3、SR、Buk、Shope、Norden、MinkⅢ、HB、F8、F9、F12等毒株的细胞培养液进行基因扩增，均获得了分子量为 2 81bp的特异性目的DNA片段，而对Vero细胞与FMDV、SVDV、HCV、PRRSV、JEV、PPV等病毒进行检测，结果均为阴性，没有出现交叉反应对PRV毒株扩增的产物测序，结果序列与文献报道一致，证明PCR扩增产物和方法的特异性对 1994～ 2 0 0 0年期间送检的临床样品和保存的PRV毒种，用病毒分离、双抗体夹心ELISA和PCR等 3种方法进行检测，结果前 2种方法检测为阳性的，PCR检测均为阳性；PCR检测为阴性，前 2种方法检测也为阴性；可是，前 2种方法检测为阴性的，PCR却检测出部分阳性；经x2 检验，证明PCR检出率明显高于前 2种方法的检出率对PRV闽A株细胞毒提取物DNA进行检测，其最低检出量为 15 8pg 对 1999～ 2 0 0 0年期间广东、福建、海南等省的 31个大中型猪场送检的 191份病料进行检测。

J. Biol. Chen., 1995, 270:17908～17912.［10］Wioto A, Baltimmore D.πρ Lineage-specific expression of the π T cell receptor gene by nearby silencer.

在很多情况下，细胞不具备与这段保守序列结合的反式因子，这时，这段保守序列就不表现调控活性。

L By looking at the individual elements in any given backbend—such as examining the direction of movement of the shoulders, arms, torso, hips and legs—we can deconstruct the posture and aligning, strengthening and opening these individual regions to more refined by identifying particular areas of interest need in our students' bodies and focusing on these areas in class, I call this "progressive sequencing" and it is different from "pose/ counter pose " sequencing.

通过关注每个后弯体式中独立的元素——例如检查肩膀、手臂、躯干、髋以及两腿的运动趋势——我们能够通过分解的方式来探究体式和学习校准；通过了解学生的身体中需要深化和发掘的独立的区域，强化并打开这些独立的区域，并且能够在课堂中专注于这些区域，我把这个叫做&渐进序列&，这与&体式/反体式&序列是不同的。

By looking at the individual elements in any given backbend—such as examining the direction of movement of the shoulders,击 arms,击 torso,刨 hips and legs—we can deconstruct the posture and aligning,凋 strengthening and opening these individual regions to more refined by identifying particular areas of interest need in our students' bodies and focusing on these areas in class,刞 I call this "progressive sequencing" and it is different from "pose/ counter pose " sequencing.

通过关注每个后弯体式中独立的元素——例如检查肩膀、手臂、躯干、髋以及两腿的运动趋势——我们能够通过分解的方式来探究体式和学习校准；通过了解学生的身体中需要深化和发掘的独立的区域，凌强化并打开这些独立的区域，凚并且能够在课堂中专注于这些区域，凟我把这个叫做&渐进序列&，凛这与&体式/反体式&序列是不同的。刦

By looking at the individual elements in any given backbend—such as examining the direction of movement of the shoulders,删 arms,凹 torso,凨 hips and legs—we can deconstruct the posture and aligning,凟 strengthening and opening these individual regions to more refined by identifying particular areas of interest need in our students' bodies and focusing on these areas in class,凝 I call this "progressive sequencing" and it is different from "pose/ counter pose " sequencing.

通过关注每个后弯体式中独立的元素——例如检查肩膀、手臂、躯干、髋以及两腿的运动趋势——我们能够通过分解的方式来探究体式和学习校准；通过了解学生的身体中需要深化和发掘的独立的区域，凨强化并打开这些独立的区域，凣并且能够在课堂中专注于这些区域，凡我把这个叫做&渐进序列&，凷这与&体式/反体式&序列是不同的。凯

By looking at the individual elements in any given backbend—such as examining the direction of movement of the shoulders,凸 arms,凊 torso,凳 hips and legs—we can deconstruct the posture and aligning,刨 strengthening and opening these individual regions to more refined by identifying particular areas of interest need in our students' bodies and focusing on these areas in class,凙 I call this "progressive sequencing" and it is different from "pose/ counter pose " sequencing.

通过关注每个后弯体式中独立的元素——例如检查肩膀、手臂、躯干、髋以及两腿的运动趋势——我们能够通过分解的方式来探究体式和学习校准；通过了解学生的身体中需要深化和发掘的独立的区域，凢强化并打开这些独立的区域，凉并且能够在课堂中专注于这些区域，刁我把这个叫做&渐进序列&，刘这与&体式/反体式&序列是不同的。刓

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。