反复法

The various kinds of markers of whole blood viscosity and erythrocyte aggregation in the rats were measured with auto-viscometer,and the maximum rate of platelet aggregation was measured by turbidimetry.

采用反复注射肾上腺素和施以冰水浸泡的方法制作急性血瘀证大鼠模型，以全自动血流变学仪测定全血粘度和红细胞聚集指数等指标，以比浊法测定血小板最大聚集率。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

Methods The skeletal muscle stem cells were enzymatically isolated with collagen and dispase, and then purified by different adhesion time from 6 female SD rats. The purified cells were transinfected with adenovirus carrying lac-Z gene, and injected into the bladder neck of 6 autologous and 4 allogeneic rats. Tissues of blader neck and ureter were obtained in sacrified rats after 5 and 15 days, histological examination and X-gal staining were performed.

采用混合酶消化及反复差速贴壁法分离、纯化培养SD大鼠骨骼肌干细胞，然后用携带lac-Z基因的腺病毒载体转染肌干细胞，待转染成功后将携带lac-Z基因的骨骼肌干细胞注射于自体和同种异体的膀胱颈及后尿道周围，分别于注射后5、15d，处死大鼠取出膀胱颈及后尿道，行组织学检查及X-gal染色。

Objective:By histochemical method, the four biotinylated lectins (DBA, SBA, WGA, UEA 1) were used to study changos in glycoconjugate expression on the surface of endometrium of 28 cases of RSA without obvious reasons in secretary phase, at the same time, endometrial biopsies were taken for histomorphologic evolution.

本文通过生物素分别标记的双花藕豆凝集素、大豆凝集素、麦胚凝集素及荆豆凝集素（UEA－1）与子宫内膜的反应，应用S－P法研究28例无明显原因反复流产的分泌期子宫内膜糖复合物的变化及与其组织形态学分期的相关性。

The essay also treats significance of this method in maths of high school education. It puts forward teaching strategy about how to emphasize maths transformation method, that is to place emphasis on basic knowledge, perfect knowledge structure, develop the transformation method, and improve the ability of transformation. We also base on our text books, and epurate and summarize the basic teaching strategy in our work.

接下来，论述了化归思想方法在中学数学教学中的意义，最后结合个人教学实践，提出了加强数学化归思想方法的教学策略，即夯实基础知识，完善知识结构；培养化归意识，提高转化能力；掌握化归的一般方法；强化通性通法教学；深入教材，反复提炼与总结等基本教学策略。

Through trial-and-error, you will know exactly how much to give them at feeding time.

透过反复试验法，你将知道在喂食时间究竟该给多少饲料。

One of the suites, for example, exhibits the musical refrain B-flat, A, C, B, which in German usage spells Bach.

比如组曲中其中一个曲子有一个&降B，A，C，B&的反复，而这四个字母排列恰巧是&巴赫&的德语拼法。

Coning and quartering method: put the sample onto the steel plate in the form of a cone; each scoop shall be inserted from the top of conical to make it spread along the top evenly, and pay attention not to misplace the center of cone; do it at least 3 times and mixed thoroughly; then flatten the top and use the cross plate from top to bottom to divide into 4 equal segments, randomly take out 2 oppositesegments and do it repeatedly for several times and finally divided into not less than the minimum retained quantity.

圆锥四分法：将样品置于已用样品刷好的钢板上，堆成圆锥形，每铲由圆锥顶尖落下，使其均匀地沿锥尖散落，注意勿使圆锥中心错位，如此反复至少移堆三次，使其充分混匀，然后将锥尖压平，用十字板自上插下，分成四等份，任取两个对角的等份，重复操作数次，缩分至不少于最少留样量。

Main point: The strength should be well-distributed and gentle, The frequency should be moderate, Holding and rubbing on joint of knees boing over two palms should be stopped on the knee with rubbing repeatedly for 5-10 seconds which main point is well-distributed and soft.

要领：用力要均匀，柔和，频率要适中，抱揉膝关节，在上述动作完毕后，顺势用两手抱住其膝关节处，用揉按法反复操作约五至十秒，其要领：均匀，和缓。

Methods Through repeated testing and practical application, the ways of "3-lines" is used in the ground precession standardization. The first line is guard line of the left marginal of the shelter, the second is allocation line of the end part of the shelter and the third is front wheel alignment line of the whole unloading vehicles.

通过反复实验测量和实际应用，采用&三线&法在场地先行标定，第1条线是方舱左侧边缘的标志线，第2条线是方舱尾部定位线，第3条线是整装整卸车前轮定位线。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。