反向

The method is just like the QuikChange site-directed mutagenesis method except for its special primer. This pair of primers is composed of a long primer and a short primer which is central overlapped.Both of the Tm of 5\'sequence in long primer and Tm of 3\'sequence in short primer are above 66℃.The Tm of short primer is almost half of that of long primer.It is convenient to perform insertion or substitution mutation of DNA fragments larger than 20bp,In addition,it is highly applicable in research about the deletion mutation of DNA fragments larger than 2000bp.

我们实验室一直在寻找一种简单高效的可以用于基因的删除、插入及替代突变的方法，通过大量研究和实验发现，可以使用一种基于反向PCR原理，使用全新的引物设计的突变方法——&COP&突变法，该方法采用与QuikChange点突变法相同的操作步骤，其策略是设计一对中心重合的引物，长引物的5\'端序列和3\'端序列的Tm值均不低于66℃，短引物Tm值约为长引物一半，且与长引物中心重合互补。

Usually, the target sequences are palindromes, meaning they read the same backward and forward.

通常，它们的目标序列具有回文结构，即从反向读和从正向读它们都是一样的。

In a VLIW processor implementation, decoding of one token is pipelined with the inverse scan and inverse quantisation step of the preceding token.

在VLIW处理器实现中，一个标记的解码与前一标记的反向扫描和反量化步骤被流水线处理。

First, we MUST add an horizontal stabilizer. This stabilizer will create an opposite moment to reduce the roll.

第一，我们必须加装水平尾翼，水平尾翼产生的反向扭矩能降低旋翼机翻滚的几率。

The results showed that activities of the forward sequence of both genotypes were higher than that of reverse sequence. Promoter activity of the recombinants with A allele was about one third lower than that with C allele.

实验结果表明，2种基因型的正向序列启动子活性均高于相应的反向序列，含有A等位基因的启动子片段活性比含有C等位基因的片段低约1/3。

A double helical gear is equivalent to two helical gears of opposite hand, mounted side by side on the same shaft.

双斜齿轮是与反向的并排地装在同一轴上的两个斜齿轮等效。

Applying E-commence strategy will lead to a negative effect on firm's FP.

电子商务策略的运用会对要素生产力产生反向的效果。4。

Reverse terminations are required for the analog input.

50Ω反向端接是必需的模拟输入。

The ray-path integrated growth is much smaller than that in electrojet conditions for the same values of driven electric field |ED| because the vertical component of group veloci...

在这一高度范围，波可能是不饱和的，增大L_N，使波在h=105km饱和的临界电场│E_D│值减小。上述计算结果可解释某些在反向电射流条件下，在Addis-Ababa观测到的10m波长雷达回波的特性。

Bottom sediement classification by using the multibeam echo sounder data is a topic of interest to marine geologist, and is also a research hotspot of application of multibeam echo sounder data.

摘要利用声反向散射数据作海底沉积物分类，是海洋地质学家感兴趣的话题，也是目前多波束声纳应用的一个研究热点。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。