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Sequences of modified genes GOX and CP4-EPSPS in GM canola and that of modified gene Cryla in GM cotton were decoded and the conservative sequences of exogenous resistant genes: PLRVrep, PVYcp and CryⅢA in GM potato: New leaf〓 PLUS and New leaf〓 Y were confirmed using the modified exogenous gene decoding technique and isogenous sequence similarity BLAST analysis. Bases on DNA sequences studied above, conventional PCR primers were designed and selected in an optimized way and the conventional PCR detection protocol for exogenous resistant genes in 19 GM crops was established.

本研究采用反向PCR克隆—测序测定未知序列技术,针对转基因玉米(MON810、BT11、BT176、GA21、T25、CBH-351)六个品系、转基因大豆GTS 40-3-2品系、转基因油菜RT73和MS8两个品系、转基因棉花MON531和MON1445两个品系测定出品系鉴定的边界序列;采用外源修饰基因序列的破译方法和BLAST同源性序列分析技术,针对转基因作物中修饰的外源抗性基因进行破译研究,破译出转基因油菜中修饰的GOX基因、修饰的CP4-EPSPS基因和转基因棉花中修饰的Cry1A基因的序列,并确定了转基因马铃署New leaf〓PLUS和New leaf〓Y两个品系中PLRVrep、PVYcp和CryⅢA外源抗性基因保守序列。

The result makes clear, xiang Di decides size of grain of course complex Mg2 jumbly (100~600 Nm), grain of retrorse titrate complex slants small (200 Nm controls), distributinging concentration of diameter of bead of grain of two-way titrate complex (400 Nm controls).

结果表明,正向滴定过程合成的Mg2晶粒大小混杂(100~600 nm),反向滴定合成的晶粒偏小(200 nm左右),双向滴定合成的晶粒粒径分布集中(400 nm左右)。

Result 1 Magnetic nanoparticles, magnetic nanoparticles modified with antisense oligodeoxynucleotide of human telomerase reverse transcriptase induced HL-60 tumor cells to apoptosis, we could see typical morphologic change of apoptosis cells: karyopyknosis, chromation"s condensing and aggregation in nuclear, forming crescent-shaped or annulus structures to lean on edge of cell nucleus"s membrane and posing apoptosis body by Atomic Force Microscope, Fluorescence microscope, transmission electron Microscope 2 There was a significant difference compared with control group(p.01), inhibition ratio had significant positive correlation with medication dosage and time ;during 0.8-8μM dosage amplitude, inhibition ratio accrescenced by dosages increasing. However, the inhibition ratio would decrease when dosage over 8-80μM.

结果 1 磁性纳米粒子、修饰有端粒酶反义寡核苷酸的磁性纳米粒子诱导HL-60细胞发生凋亡,原子力显微镜、光学显微镜、荧光显微镜和透射电镜下均观察到HL-60细胞呈现典型的凋亡细胞的形态变化:细胞核固缩,核内染色质浓缩、凝聚、形成新月形或环状结构紧靠在细胞核膜边缘,并形成凋亡小体。2 磁性纳米粒子、修饰有端粒酶反义寡聚脱氧核苷酸的磁性纳米粒子对HL-60肿瘤细胞的生长和增殖有明显的抑制作用,与对照组相比有显著性差异(p<0.01),在剂量为0.8-8μmol/L范围内,抑制率随剂量的增加而增加,当剂量超过8μmol/L时,抑制率反而下降;3 磁性纳米粒子、修饰有端粒酶反义寡聚脱氧核苷酸的磁性纳米粒子可增强p53基因的表达活性,引起DNA降解损伤,反向调节细胞周期活动,促使细胞从G0期进入G1期,抑制肿瘤细胞的生长。4 修饰有端粒酶反义寡聚脱氧核苷酸的量子点能通过内吞作用进入HL-60肿瘤细胞的细胞核,可以在细胞内进行定位和促进HL-60肿瘤细胞的凋亡。

Nevertheless Kees van den Ende, the reverse-electrodialysis project manager at KEMA, thinks his process has some advantages over the Norwegian way (which is known, technically, as pressure-retarded osmosis).

但是KEMA公司反向电渗析项目的负责人基斯·范登·恩德认为他们的方法相对于挪威的方法(用科学术语来说,叫作&压力延迟渗透&)来说有几个优点。

The sense of vertical throw is thus opposite to the general NW sideup thrusting of the Wenchuan rupture. We propose that it is due to the inheritance at shallow depth of the SEdipping fault of the klippen system in the south.

本文对高原新村附近的反向陡坎进行了深入的探讨,并综合前人的研究结果,认为是破裂在从深部往上扩展的过程中,在浅表部位顺着发育在虹口东南部的大鱼洞-龙溪飞来峰后缘的具南东倾向的断面继承而成。

This thesis sets up a new method to synthesize stereoselectively ?ra玸--2,5-disubstituted tetrahydrofurans by introducing two alkyl groups in 3-C and 5-C of lactol, flash decomposition and hydrogenation.

本文独创新的合成方法:以-乳醇,即-4,10-二氧杂三环[5.2.1.0~(2,6)]-癸-8-烯-3-醇为原料,在其3-位和5-位先反向引入两个取代基,再经过热分解和催化加氢反应,高立体选择性地合成反式-2,5-二取代四氢呋喃。

The key problem is to synthesize high stereoselectively trans- 2,5- disubstituted tetrahydrofurans. This thesis sets up a new method to synthesize stereoselectively ?ra玸--2,5-disubstituted tetrahydrofurans by introducing two alkyl groups in 3-C and 5-C of lactol, flash decomposition and hydrogenation.

本文独创新的合成方法:以-乳醇,即-4,10-二氧杂三环[5.2.1.0~(2,6)]-癸-8-烯-3-醇为原料,在其3-位和5-位先反向引入两个取代基,再经过热分解和催化加氢反应,高立体选择性地合成反式-2,5-二取代四氢呋喃。

Objective To observe the effect of complications such as middle turbinate drift and adhesion in the treatment of middle turbinate dealtwith functional endoscopic sinus surgery.Methods In160cases dealt with FESS,to seek for what happened about the complications by resecting the lateral section and/or basilar section of middle turbinate,and keep its plate continuance by resecting the posterior ethmoid when the middle turbinate has been found to be laterotorsion,reverse,hypertrophy or vesicle change.

目的 观察实施功能性内镜鼻窦手术时,中鼻甲的处理对中鼻甲漂移、粘连并发症的影响方法在160例患者实施FESS时,对中鼻甲外翻、反向、肥、泡性改变,实施中鼻甲外侧或并游离缘部分切除,保持中鼻甲基板连续性,观察中鼻甲漂移、粘连并发症发生情况。

Methods In160cases dealt with FESS,to seek for what happened about the complications by resecting the lateral section and/or basilar section of middle turbinate,and keep its plate continuance by resecting the posterior ethmoid when the middle turbinate has been found to be laterotorsion,reverse,hypertrophy or vesicle change.

在160例患者实施FESS时,对中鼻甲外翻、反向、肥大、泡性改变,实施中鼻甲外侧或并游离缘部分切除,保持中鼻甲基板连续性,观察中鼻甲漂移、粘连并发症发生情况。

However," two-dimensional letoff/handover paper " rarely used at the time of General letoff, you must have business card printing and membership card making faces, which is a one-way process.

必然必要在第1个单位举行背后制卡和会员卡制作,这时不离请求制卡和会员卡制作机可以反向不播卷和移接纸。

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In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是,在否定句或疑问句里,这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷

Nusa lembongan : Bali's sister island, coral and sand beaches, crystal clear water, surfing.

Nusa Dua :豪华度假村,冲浪和潜水,沙滩,水晶般晶莹剔透的水,网络冲浪。