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Then the promoter CaMV 35S driven, plant siRNA expression vector pARB with inverted repeats of BBEwas constructed based on the vectors pHANNIBAL and pART27. The work will lay the foundation for breeding a low morphine and high thebaine poppy.

以中间载体pHANNIBAL和植物表达载体pART27为基础,构建了CaMV-35S启动子驱动的含小檗碱桥酶基因片段反向重复序列的RNAi双元表达载体pARB,为培育低吗啡,高蒂巴因的罂粟新品系奠定了基础。

The cloned BBE gene sequences were 94.84% identified with the reported BBE genes in GenBank previously. Based on the cDNA sequences of COR and BBE ,two fragments about 400~500 bp from each gene with lower identity among them were cloned. The fusion gene BC (744 bp) is fused by the PCR technique. Then the promoter CaMV 35S driven, containing'forward BC fusion fragments-reverse pdk intron-reverse BC fusion fragments', plant siRNA expression vector were constructed based on the vectors pHANNIBAL and pART27.The work will lay the foundation for breeding a low morphine and high thebaine poppy.

利用blast及分子生物学软件DNAStar对COR和BBE基因的cDNA序列同源性进行分析比较,分别从各基因中筛选和克隆了一段同源性极低、约400~500 bp的片段;并应用重叠PCR法将其拼接成744 bp的融合基因BC,以中间载体pHANNIBAL和植物表达载体pART27为基础,构建了以CaMV 35S启动子驱动的含有"正向BC融合片段- pdk内含子-反向BC融合片段"的ihRNAi植物表达载体,通过转化野生罂粟,初步研究了以COR和BBE基因为靶标的RNAi对内源吗啡合成的抑制效果,为进一步培育低吗啡高蒂巴因的罂粟种质提供了依据。

Based on the thermionic emission theory, a method to extract parameters of Si SBD is given in this study. Using this model, we tested the characteristics of devices and extracted the parameters of two kinds of samples. The ideality factor is 1.01 and 2.13 respectively. The zero-field barrier height is 0.70eV and 0.72eV. The surface state density is 5.5×10~(15)cm~(-2)eV~(-1) and 4.3×10~(12)cm~(-2)eV~(-1). The interface oxide capacitance is 9.0×10~(-4)F·cm~(-2) and 5.4×10~(-6)F·cm~(-2). The neutral level of the surface states is 0.81eV and 1.1eV. The reverse breakdown voltage is 101V and 56V.

本研究在热电子发射模型的基础上,建立一种提取Si基SBD器件特性参数的理论模型,基于这个模型,对两种样品的性能进行测试,并计算出退火和未退火样品的理想因子分别为1.01和2.13、零电场势垒高度分别为0.70eV和0.72eV、表面态浓度分别为5.5×10~(15)cm~(-2)eV~(-1)和4.3×10~(12)cm~(-2)eV~(-1)、界面层电容分别为9.0×10~(-4)F·cm~(-2)和5.4×10~(-6)F·cm~(-2)、表面态中性能级为0.81eV和1.1eV、反向击穿电压101V和56V。

Feed the gross pipe from front plant, cog down the upper roller to given size to rotating bring the pipe into rolling zone for reducing diameter and wall form rolling; after rolling from head to tail, turn 90Deg with the gross pipe, top bar and core head by tilter for next rolling travel that inverse roll to head to bring the pipe back to front plant; then, turn again for next periodical to-an-fro rolling till design size request.

将毛管自轧机前台喂入,将上轧辊压下至设定的尺寸,毛管在轧辊的旋转带动下曳入轧制区域,进行减径和减壁变形轧制;毛管从头部轧制到尾部后,将毛管、顶杆及芯头在翻转装置的作用下翻转90°,进行下一个轧制行程,反向轧制到头部,毛管回到轧机前台;再将毛管、顶杆及芯头在翻转装置的作用下翻转90°,进行下一个轧制周期往复轧制,轧制到毛管成达到设计尺寸要求。

At first, this article introduces the background and main methods of DoS attacks, and discusses the advantages and disadvantages of some traditional methods for traceback.

在对节点采样标记算法分析的基础上,设计出本文自己的反向节点采样标记算法,改进后的算法在性能与效率上有显著提高。

From the point of view of original owner of a trade mark , it is a kind of trade mark tort.

从原商标权人的角度看,商标反向假冒行为是一种商标侵权行为。

So that, it's easy to deduce the correct angle by a trigonometric function equation, which can be setup using Variance derivative must be equal to zero.

利用方差导数为零建立三角函数等式,进行校正角的反向推导计算。

Using semi-quantitative RT-PCR, the differential expression profiles of eleven selected genes were confirmed in the ovaries of triploid and diploid. These genes fell in gene categories with a wide range of functions. The results indicated that triploidy affects the dynamic gene regulatory network in triploid ovary. This study established a firm basis for future investigation on characterization of crucial molecular events for normal ovarian development in shrimp.To further dissect exact gene functions for gonad development of shrimp, three differentially expressed genes between diploid and triploid ovary, PCNA (proliferating cell nuclear antigen), CAS/CSE1 (cellular apoptosis susceptibility protein/chromosome segregation 1) and SSRF (spermatogonial stem-cell renewal factor) were characterized on certain aspects.

利用抑制性消减杂交技术,建立了对虾二倍体和三倍体卵巢间的2个消减文库;在正向消减文库(以三倍体卵巢作为实验组,二倍体卵巢作为驱动组)中,鉴定到54个基因;在反向消减文库(以二倍体卵巢为实验组,三倍体卵巢为驱动组)中,鉴定到16个基因;选取11个差异表达的基因,利用半定量RT-PCR的方法对其在二倍体和三倍体卵巢间的表达进行了检测,均能很好地与消减结果相吻合;这些差异基因编码多种功能的蛋白,分析表明染色体的三倍化使三倍体卵巢中的基因调控网络受到了影响;为深入揭示维持卵巢正常发育的关键分子调控事件奠定了基础。

Build suspense as you chase your victim in this fast paced 2d reverse tron.

制造悬念,你追逐你的受害者反向特隆在这个快节奏的2D。

The black curve shows the response with the tweeter connected with opposite polarity.

图里的黑色曲线显示了极性反向相连的高音喇叭响应。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

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A single payment file can be uploaded from an ERP system to effect all pan-China RMB payments and overseas payments in all currencies.

付款指令文件可从您的 ERP 系统上传到我们的电子银行系统来只是国内及对海外各种币种付款。