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Reverse transcription polymerase chain reaction and western blot were used to detect the COCH and POU3F4/brn4 gene's expression. To investigate in vitro characteristics of COCH gene's expession, two factors were introduced in the culture medium of spiral ligament type Ⅰ fibrocytes respectively. Firstly, phosphorothionate-modified antisense oligodeoxynucleiotides to POU3F4/brn4 mRNA was applied to study the regulation effects of POU3F4/brn4 to COCH gene transcription.

引入人工合成的针对POU3F4/brn4基因mRNA的义硫代磷酸寡聚脱氧核苷酸,干扰耳蜗螺旋韧带Ⅰ型成纤维细胞POU3F4/brn4的表达,免疫印迹检测义干扰细胞POU3F4/brn4蛋白条带的强弱,RT-PCR检测义寡核苷酸干扰细胞COCH基因转录水平的变化,观察POU3F4/brn4对COCH基因的转录调控作用。

In order to get a clear insight into the nature of this cleaning process and provide a solid basis for the industrial applications, this dissertation focuses on the fundamental investigation of the rigid ceramic candle filter, mainly including experimental study, theoretical analysis and dynamic modeling of the unsteady flowfield in the whole pulse cleaning process.

针对刚性陶瓷过滤器在其理论和工程应用中存在的问题,本论文对脉冲吹全过程中气体引射空间和滤管内外空间的瞬态流场进行了系统的实验研究和理论分析,在此基础上进行了数值模拟并建立了脉冲吹系统数学模型,提出了改进脉冲吹清灰效果的阻尼式气体引射器。

Many new methods, such as reduction of gravity data to a horizontal plane, wavelet analysis, higher order statistics, joint inversion and intera...

山区重力资料曲化平,小波分析及高阶统计量等现代信号处理方法,重震、重磁、电震的联合演与交互演,三维可视化演,BP人工神经网络方法等在综合地球物理处理解释中得到广泛应用。

They pass Brindejonc's previous AT gun emplacement and one of the tank explodes on one of the AT mines laid there by the French gunners !

但是,R-35在通过他们的坦克阵地时,一辆坦克竟然压到了坦克手们之前埋设的坦克地雷!

In 1940, a French infantry division was made up of three infantry regiments (each one having a headquarters company, a weapons company and three battalions), one cavalry unit tasked with reconnaissance, one anti-tank company (12 25mm anti-tank guns), one light field artillery regiment with three groups of 12 75mm guns each (plus an anti-tank and anti-aircraft company to protect the field guns from enemy tanks and aircraft), one heavy field artillery regiment (two groups of 12 155mm howitzers each), two engineer companies, two signals companies, two transport companies, one ammunition company, one medical group and divisional services.

在1940年,一个法军步兵师由三个步兵团(每个都有一个司令部,一个武器连和三个营),一个负责侦察的骑兵部队,一个坦克连(12架25mm坦克炮),一个轻型野战炮团配备有12架75mm炮三组(再加上一个坦克防空连保护野战炮不受敌人坦克和飞机的攻击),一个重型野战炮团(12架155mm榴弹炮两组),两个工兵连,两个通信连,两个运输连,一个军火连,一个医疗大队和师后勤部门。

It is the preprocessing of the Original data of the seismic inversion, the main control factor analysis, of affecting inversion results, and the geologic explain ways of the inversion results that is Point discussed.

重点讨论了地震演原始资料的预处理、影响演结果的主要因素和演结果的地质解释方法。

A novel least squares algorithm based on anti-WKB method and linear segment interpolation,which is called prediction-verification method, is proposed.This method takes the values calculated by anti-WKB and linear segment interpolation as approximation,then uses the least squares fitting,and after that compares the linear segment interpolation based on the anti-WKB method with it.

提出一种基于WKB法与分段线性插值的最小二乘拟合方法来确定离子交换玻璃波导的横向折射率分布,它将WKB法与分段线性插值所计算出的结果作为近似值,然后进行最小二乘拟合,并与基于WKB的分段线性插值方法进行比较。

It is concluded that because the mica schist is mixed with feeble weathered granite, the grain is broken up severely; the back-analysis parameters of explosion rockfill(3BⅠ)are lower than the results of laboratory triaxial test, and the Duncan E-B parameters obtained by back-analysis of her sandy gravel (3BⅡ)are higher than the triaxial test results of laboratory triaxial test which can not reflect the mechanical behaviors of the dam material objectively; the research should be strengthened in 300 m high rockfill dam construction. The fitting modes of the space displacement field of prototype observation are also explored and it turns out that the results of IGA analysis considering only vertical deformation meet the need of dam's technical accuracy.

结果表明,由于在微弱风化花岗岩掺加云母片岩,颗粒破碎严重,爆破堆石料(3BⅠ)的原级配粗粒料演参数远低于室内三轴试验成果;与3BⅠ堆石料相,砂砾料(3BⅡ)现场演得到的邓肯E~B模型参数高于室内三轴试验值;室内三轴试验成果不能客观映筑坝材料的力学特性,对于当今的300 m级高土石坝建设,宜加强对原级配粗粒料的力学特性研究;同时对原型实测的空间位移场不同拟合方式进行了探讨,认为仅考虑铅直向变形的IGA参数演成果能基本满足土石坝工程的精度要求。

Firstly the buried depth and distribution of the Pre-Silurian interface were in- versed from magnetotelluric data with constraint conditions.

从提高纵向分辨率的处理和演技术的应用入手,结合约束条件,利用大地电磁测深数据演,揭示前志留面等界面的埋深和分布;同时应用小波分析技术,从磁场中分解出区域磁异常,演结晶基底的埋深。

The method is easy to utilize, and it only needs spectral data of two different kinds of land covers. In addition, it doesn't need the albedo computation of narrow wavebands. The method proves to be a good means for inversion of surface albedo.

该方法不但简便易行,所需野外数据较少,物理意义明确,而且省去了窄波段照率的计算过程,是一种遥感演区域照率的好方法。

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Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车,和一双合并为一移交&整个lotta爱&。

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