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Flow cytometry was used to measure the cells cycle kinetics of the treated cells. Hoechst33258/PI double staining was performed to evaluate the cell morphology. Western blotting was conducted to analyze the expressions of p53, Bcl 2 and Bax. Results 2 ClAdo and 2 CldAdo had different cytotoxicities on the cells, while 2' dAdo had no cytotoxicity on the cells.

以胎盼蓝活细胞拒染法测定药物对细胞生长、增殖的抑制及IC50 ;以流式细胞术观察药物对细胞周期的影响;以荧光双染法(Hoechst33258/PI)观察药物对细胞形态学的影响;以Western blot法观察药物对细胞P53、Bcl 2、Bax蛋白表达的影响。

METHODS: Bone of limbs were obtained under sterile condition, washed by PBS, centrifugalization, and dropwise into centrifuge tube containing 1.073 g/mL Percoll separating medium, centrifugalization, and then adding L-DMEM containing 10% fetal bovine serum, benzylpenicillin sodium and phytomycin, cultured in a incubator at 37 ℃ with 5% saturated humidity. The culture medium was renewed after 48 hours, and non-adhesive cells were removed.

无菌条件下截取流产胎儿双侧四肢骨,PBS冲洗髓腔,离心去脂肪及上清液,L-DMEM重悬细胞,沿管壁缓慢滴加至底部有等量1.073 g/mL Percoll分离液的离心管中,离心后吸取中间界面白膜层,加入含体积分数为10%的胎牛血清、青霉素钠、链霉素的L-DMEM完全培养基,接种后置于37 ℃、体积分数为5%的CO2饱和湿度孵箱内培养。48 h后换液,去除未贴壁细胞,以后每2 d换液一次。

The puerperant and her relatives all signed informed consents. METHODS: Bone of limbs were obtained under sterile condition, washed by PBS, centrifugalization, and dropwise into centrifuge tube containing 1.073 g/mL Percoll separating medium, centrifugalization, and then adding L-DMEM containing 10% fetal bovine serum, benzylpenicillin sodium and phytomycin, cultured in a incubator at 37 ℃ with 5% saturated humidity.

无菌条件下截取流产胎儿双侧四肢骨,PBS冲洗髓腔,离心去脂肪及上清液,L-DMEM重悬细胞,沿管壁缓慢滴加至底部有等量1.073 g/mL Percoll分离液的离心管中,离心后吸取中间界面白膜层,加入含体积分数为10%的胎牛血清、青霉素钠、链霉素的L-DMEM完全培养基,接种后置于37 ℃、体积分数为5%的CO2饱和湿度孵箱内培养。48 h后换液,去除未贴壁细胞,以后每2 d换液一次。

Dizygotic twin pregnancies account for about two-thirds to three-quarters of all spontaneous multiple pregnancies in a random population, therefore the results of this study predominantly, but not exclusively, represent fraternal twins.

因为在随机人群的自发性多胎妊娠中,双合子双胞胎妊娠占了大约2/3到3/4,所以本研究主要反映了异卵双胞胎的情况。

The vascular bundle of stem is the type of double phloem. The anther is tetrasporangiate. Simultaneous cytokinesis in the microspore mother cell follows meriosis.

茎的维管束是双韧类型;雄蕊的花药四室,小孢子的形成为同时型胞质分裂,悬垂胎座,倒生胚珠,核型胚乳,果实成熟时胚仍处于球形胚阶段。

Shadowless sword manual is the Massachusetts Institute of Technology successfully developed the world's most advanced technology, a high-pressure injection technology, at 0.01 seconds of time to quickly solve a variety of symptoms of aging nano trace elements, in order to fog-state in the form of fixed-point, quantitative, fixed layer, positioning, in order to fan-shaped form of the direct, accurate tire reaches the skin layer, 20-minute onset, 2 hours effective, and 48 hours markedly effective, non-invasive no side effects, can wipe out wrinkles, Sichuan word pattern, raised eyebrow, raised his eyes, his eyes cured pattern, eyes with a black eye, heal nasolabial fold, modified nose shape, face-lift, facial upgrade, double chin, carved facial contours, 7 days to eliminate fat, slim build remodeling , no recovery of cotton contour sculpture mm accuracy can be controlled, not only to solve the aging problem, had a beautiful face can be more delicate to maintain years.

无影刀说明书是美国麻省理工大学研制成功目前世界上最先进的技术,一种利用高压喷射技术,在0.01秒的时间内快速将能解决各种不同衰老症状的纳米微量元素,以雾态的形式定点、定量、定层、定位,以扇状的形直接、准确的到达皮肤中胎层,20分钟起效,2小时见效,48小时显效,无创无副作用,可祛除皱纹、川字纹、提眉、提眼角、祛眼角纹、眼带、黑眼圈、平复鼻唇沟、修饰鼻形、瘦脸、面部提升、双下巴、精雕面部轮廓,7天之内消除脂肪,重塑苗条身材,无恢复期,对棉布轮廓雕塑精准度可控制的毫米之间,不光是解决了衰老问题,可使原本漂亮的脸更加精致,维持之久。

Shadowless sword manual is the Massachusetts Institute of Technology successfully developed the world's most advanced technology, a high-pressure injection technology, at 0.01 seconds of time to quickly solve a variety of symptoms of aging nano trace elements, in order to fog-state in the form of fixed-point, quantitative, fixed layer, positioning, in order to fan-shaped form of the direct, accurate tire reaches the skin layer, 20-minute onset, 2 hours effective, and 48 hours markedly effective, non-invasive no side effects, can wipe out wrinkles, Sichuan word pattern, raised eyebrow, raised his eyes, his eyes cured pattern, eyes with a black eye, heal nasolabial fold, modified nose shape, face-lift, facial upgrade, double chin, carved facial contours, 7 days to eliminate fat, slim build remodeling , no recovery of cotton contour sculpture mm accuracy can be controlled, not only to solve the aging problem, had a beautiful face can be more delicate to maintain years.

去 美容美发保健品 IM互动商圈,与商家群聊发现更多商机。共有24条同类&美体器材&信息,请通过分页查看 12 3 4 5 无影刀说明书是美国麻省理工大学研制成功目前世界上最先进的技术,一种利用高压喷射技术,在0.01秒的时间内快速将能解决各种不同衰老症状的纳米微量元素,以雾态的形式定点、定量、定层、定位,以扇状的形直接、准确的到达皮肤中胎层,20分钟起效,2小时见效,48小时显效,无创无副作用,可祛除皱纹、川字纹、提眉、提眼角、祛眼角纹、眼带、黑眼圈、平复鼻唇沟、修饰鼻形、瘦脸、面部提升、双下巴、精雕面部轮廓,7天之内消除脂肪,重塑苗条身材,无恢复期,对棉布轮廓雕塑精准度可控制的毫米之间,不光是解决了衰老问题,可使原本漂亮的脸更加精致,维持之久。

To explore how p110C participated in apoptosis, we used p110C as bait in yeast two-hybrid system screens of a human fetal liver cDNA library, and identified p21-activated kinase 1 (PAK1) as a partner of p110C. The interaction of PAK1 and p110C was specific because neither interaction between PAK1 and other PITSLRE kinases nor interaction between p110C and other Group Ⅰ PAKs (PAK2 and PAK3) was detected.

为了寻找p110C参与细胞凋亡的可能机制,我们以p110C为诱饵蛋白,用酵母双杂交技术筛选人胎肝cDNA文库,找到了与p110C结合的蛋白PAK1.p110C与PAK1的相互作用是特异性的,这是因为我们既没有检测到p110C与其它Ⅰ类PAKs的相互作用,也没有检测到PAK1与其它PITSLRE激酶成员的相互作用。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。

Closes the eye, the deep breathing, all no longer are the dreams as if......

关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。