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Studies showed that the Giemsa-C banding patterns of Ae.tauschii- H.vulgare amphidiploid is much in accord with that of its two parents, and this further proved that the regenerated plants from embryo callus of hybrids between Ae.tauschii and H.vulgare (2n=28) were spontaneously chromosal doubling amphidiploid.

分析表明,节节麦-大麦双二倍体Giemsa--C带核型与两个亲本的带型基本一致,这证明了节节麦-大麦双二倍体的染色体组成。

Bnormal MRI manifestations in all of the 20 cases with acquired hepatocerebral degeneration,in which T1WI demonstrated increased signal in hibateral lentiform nucleus (n=20),globus pallidus(n=17),surrounding mesencephalon red nucleus(n=15),and in anterior pituitary(n=4)While T2WI demonstrated no corresponding alteration in signal intensityConclusion Abnormal MR signal in the patients with acquired hepatocerebral degeneration has special features and MRI has right value for the diagnosis of this disease

全部病例均有完备的临床与MRI资料。结果:20例脑部均有阳性MRI表现,其中双侧、豆状核20例,苍白球17例,中脑红核周围15例,垂体前叶4例,表现为自旋回波序列T1WI为高信号,T2WI及FLAIR像无异常。结论:获得性肝性脑部变性的MRI征象具有特征性,MRI对该病的诊断有较高价值。

The DSC results showed that calcium carbonate,sodium benzoate and terephthalic acid were the nucleating agents of polypropylene with high thermal stability of the nucleating effect,while the thermal stability of nucleating effect of pimelic acid,suberic acid,azelaic acid,and the two component nucleating agents suberic acid/calcium carbonate and azela...

研究了碳酸钙、苯甲酸钠、对苯二甲酸和脂肪二元酸:庚二酸、辛二酸、壬二酸及其与碳酸钙组成的双组分成核剂对聚丙烯结晶成核效果的热稳定性。

Based on the research on the solidifiation of twin-roll casting strip,the analytical model and emulational model of nucleation,the growth of dendrity tip and colunar dendrity transformation to equiaxis dendrityof twin-roll casting strip solidification are established by means of the principle of metal solidification and modern computer emulational technology.

在研究双辊薄带连铸工艺凝固过程的基础上,运用金属凝固的基本原理,并用现代计算机仿真技术建立了双辊连铸薄带凝固的形核、枝晶尖端的生长动力学、柱状晶向等轴晶生长的转变的解析模型及仿真模型,为双辊连铸薄带凝固组织形成的仿真模拟奠定了基础。

Then pMD-P80 is digested by the enzymes Xho I and Apa I, separated and linked to the linear plasmid pEGPF-C1 after digested by Xho I and Apa I. So the recombined expression plasmid pGFP-P80 is achieved. Then it is sequenced, PCR and digested by the restriction enzymes Xho I and Apa I. The results give that pGFP-P80 is achieved successfully and the insert sites, direction and reading frame are all right. It builds the basis for expressing, purifying, gaining p80 protein from mammiferous cells.

将pMD-P80 分别经Xho I 和Apa I 双酶切和回收,然后与经过Xho I 和Apa I 酶解的真核表达载体pEGPF-C1 连接、转化,获得重组质粒,经PCR,Xho I 和Apa I 限制性酶切和序列测定,鉴定为真核表达质粒pEGFP-P80,并且目的基因的插入位置、方向和读码框完全正确,为在哺乳动物细胞中表达并分离纯化p80 蛋白奠定了基础。

Results The micronuclear rate in medium dosage group and high dosage group were significantly higher than that of control group(P<0.01). The ratio of sperm malformation in middle group and high group were higher than those of controls (P<0.01).The ratio of sperm malformation increased in dose-dependent manner with dose, a dose-response relationship was found. Conclusion The DBP contamination presents the positive results under the certain dosage to the mouse marrow micronucleus experiment and the spermatozoon abnormal experiment.

结果 中、高剂量组微核率与阴性对照组相比明显升高(P<0.05),且各剂量组微核率随染毒浓度的增加而升高,呈现剂量-反应关系(r=0.937,P<0.01);中、高剂量组精子畸形率与阴性对照组比较差异有统计学意义(P<0.05),且各剂量组精子畸形率随染毒浓度的增加而升高,呈剂量-反应关系(r=0.904,P<0.01),畸形精子中以无钩为最多,其次是香蕉形、尾折叠、双头,分别为4.5%、3.09%和1.32%,结论 DBP染毒在一定剂量下对小鼠骨髓微核试验和精子畸形试验呈现阳性结果。

The microkemel and sperm cell abnomalities were analyzed. Results The micronuclear rate in medium dosage group and high dosage group were significantly higher than that of control group(P<0.01). The ratio of sperm malformation in middle group and high group were higher than those of controls (P<0.01).The ratio of sperm malformation increased in dose-dependent manner with dose, a dose-response relationship was found. Conclusion The DBP contamination presents the positive results under the certain dosage to the mouse marrow micronucleus experiment and the spermatozoon abnormal experiment.

结果 中、高剂量组微核率与阴性对照组相比明显升高(P<0.05),且各剂量组微核率随染毒浓度的增加而升高,呈现剂量-反应关系(r=0.937,P<0.01);中、高剂量组精子畸形率与阴性对照组比较差异有统计学意义(P<0.05),且各剂量组精子畸形率随染毒浓度的增加而升高,呈剂量-反应关系(r=0.904,P<0.01),畸形精子中以无钩为最多,其次是香蕉形、尾折叠、双头,分别为4.5%、3.09%和1.32%,结论 DBP染毒在一定剂量下对小鼠骨髓微核试验和精子畸形试验呈现阳性结果。

The gray value of SP and CGRP radioactive particles in bilateral spinal trigeminal caudal subnucleus in rats were detected by immunohisto- chemisrty while obvious damages in trigeminal neurocytes on the injected side were confirmed.

在确认实验侧三叉神经节细胞出现明显破坏的同时,采用免疫组织化学技术,检测大鼠双侧三叉神经脊束核尾侧亚核内P物质和降钙素基因相关肽免疫阳性颗粒的灰度值。

Morphologically and biologically, the triple-layered rotavirus particles are composed of three protein capsids, outer capsid includes VP4 and VP7, the middle is VP6, and the core is composed of VP2. VP1, VP3 and the 11 segments of double-stranded genomic RNA.

完整的轮状病毒颗粒直径约70nm,为正二十面体结构,由三层蛋白壳包绕,外壳为VP4及VP7蛋白,VP6蛋白构成病毒第二层蛋白壳,最内层为核壳,由结构蛋白VP1、VP2、VP3组成,核壳内包含有11个分节段的病毒双链RNA基因片段。

The genes of hammerhead ribozymes targeted against two and three sites on negative strand of potato spindle tuber viroid were designed, synthesized and cloned according to the action manner of hammerhead ribozyme.

根据锤头型核酶的作用模式,设计、合成并克隆了特异切割马铃薯纺锤形块茎类病毒负链RNA不同区域位点的双价和三价锤头型核酶基因。

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