双核的

Double fertilization The fusion of one pollen nucleus with the egg to form the zygote and of the other pollen nucleus with a polar nucleus to form the triploid endosperm nucleus.

双受精：指花粉粒中的一个精子与胚珠中的卵细胞结合形成受精卵，另一个精核与极核结合形成三倍体的胚乳核。

When EMS was applied, it was found that 2.0% EMS resulted the highest microkernel rate (15.6‰). Treating the materials with 2.0% EMS for 4 hours could lead to semi-lethal. Cytological observations found that chromosome aberration phenomena such as single microkernel, double microkernel, three microkernel, chromosome bridge, resort chromosome and syncytium were presented in the mutants.

EMS诱变途径中，浓度为2.0 %处理时间为4 h的组合获得最高的微核率（15.6‰），EMS浓度为2.0 %处理时间为4 h的组合使材料达到半致死；细胞学的观察看到了单微核、双微核、三微核、染色体桥、滞留染色体和合胞体等染色体畸变现象，再生苗中发现了1棵变异株。60Co辐射的诱变途径中，20Gy的剂量使不定芽半致死，再生苗中发现了1棵变异株。

We are satisfied that thefive main components were pure and plenty enough for NMR study. The structure and configuration of key component of urushiol weresuccessfully determined by using 〓 and 〓 conventioanl method,DEPTtechnique,homo- and heteronuclear chemical shift correlation 2D-NMR,2D-J resolved NMR,heteronuclear relayed coherence transfer 2D-NMR,and relaxation time measurement.The assignment of resonances for 〓and 〓 were confirmed.

对中国生漆含量最大的关键漆酚组分，在〓常规NMR谱和DEPT编辑谱的基础上，采用同核及异核化学位移相关二维谱，同核J分辨谱和异核中继相干传递二维谱等2D—NMR方法，并结合弛豫时间〓测定技术等，完全确定了占中国生漆总量60%左右的有两双键共轭的三烯漆酚的结构与构型。

The increase in either OCDAS or AAS scores associated with provocation was statistically significant (P＜0.01). A statistically significant increase (P＜0.05) in rCBV during the OCD symptomatic state versus the control state was found in the right inferiofrontal cortex, postcentral cortex, angular cortex, and bilateral orbitofrontal cortices, cuneus cortices, heads of caudate neucli, thalami and amygdalae.

结果 静息期和诱发期的强迫症状评分结果分别是1.00±0.33和6.65±0.71，有显著性差异（P＜0.01）；静息期和诱发期的焦虑症状评分结果分别是1.20±0.42和2.75±0.42，也有显著性差异（P＜0.01）；与静息期相比，右侧额下回、中央后回、角回、双侧眶额部、楔叶皮质及双侧尾状核头、丘脑、杏仁核rCBV在症状诱发期显著增加（P＜0.05）。

Methods: Human T cell leukemia cell line Jurkat was chosen as a model. The effect of deguelin on the growth of Jurkat cells was studied by 3-(4,5-dimethyl-2-thiazolyl)-2,5 diphenyl-2H-tetrazolium assay. Apoptosis was detected through DNA fragmentation assay, Hoechst 33258 staining assay and Annexin V/PI double-labeled cytometry. The expression levels of nucleophosmin and nucleoporins, including Nup88 and Nup214, were studied by flow cytometry, Western Blot and reverse transcription-polymerase chain reaction.

以人类T淋巴细胞白血病细胞系Jurkat作为研究对象，采用MTT法检测细胞增殖活性；DNA ladder法、Hoechst33258染色法和Annexin V-FITC/PI双标法检测细胞凋亡；流式细胞术、Western Blot、RT-PCR检测鱼藤素作用前后，Jurkat细胞内核孔蛋白Nup88、Nup214与核磷蛋白(nucleophosmin， NPM)表达水平的变化；激光共聚焦显微技术观察上述核孔蛋白与核磷蛋白的亚细胞定位情况。

Relationship between DNA content and evolutionary degree DNA contents of bivalves were analyzed statistically with SPSS software at subclass, order, family and species grade respectively. Significant difference was detected between mean values of two subclasses according to the result of T-test. The DNA content of Heterodonta (3.77pg) was distinctly larger than that of Pteriomorphia (3.14pg). It was concluded based on ANOVA that the general trend of DNA content was to change from lower to higher values associated with evolution at higher taxonomical classification, i.e.

核DNA含量与进化地位的关系运用SPSS统计软件，在亚纲、目、科以及种4个分类等级上对双壳贝类的核DNA含量进行了统计分析。T检验结果显示，两亚纲核DNA含量的平均值有明显差异，异齿亚纲的核DNA含量平均值(3.77pg)显著大于翼形亚纲(3.14pg)，该结果表明，核DNA含量在较高的分类等级，即亚纲上表现出随着进化地位的升高显著增大的趋势。

We didn\'t find activated points in cerebellum and deeper brain.3.Acupuncture of sham point can significantly activated BA2,6,8,13,21,37,40,44,45,47,putamen and other areas.Both the left and right side of the brain have activated points,but points on the right side are more than those on the left.Both sides of middle temporal gyms,parietal lobule,supramarginal gyms and the lentiform nucleus have obvious activated points.The activated points mainly concentrated in the cerebral cortex,the deep-activated are mainly in the putamen.4. Sham needling in sham point can significantly activate BA6, 8,9,10,18,21,37,40,43,44.The activated points are mainly distributed in the right side of the brain.The left side also has some activated points;5.In the comparasion of Acupunture and sham-needling in S J5,we find that BA8 and cerebellum have distinct regional activated points;6.In the comparasion of acupuncture in SJ5 and sham point,we find BA2 and left cerebellar regions have activated points;7.Sham needling in S J5 compared with sham point,we find BA7,8,9,18 and other areas have activated points,the main activated points are at the left brain.It is not difficult to find that the distribution of activated points are mainly in the middle brain,no obvious activated points at the temporal lobe.

结果：通过对数据的处理和分析，我们初步发现：1、外关穴真针刺能显著激活Brodmann area45、37、44、40、22、13、37、47区、海马、杏仁核、黑质等区域，小脑左侧更明显，左侧颞叶皮层激活点多于右侧，且脑部左侧深层激活点多于右侧；2、外关穴假针刺能显著激活BA46、44、41、13、40、37、19区等区域，激活点主要集中表现在大脑皮层，以颞叶为主，小脑及深部未发现明显激活点；3、非穴真针刺组结果分析初步表明，BA2、6、8、13、21、37、40、44、45、47区以及壳核等区域有激活点，大脑左、右侧均有激活点，但右侧更多，双侧颞中回、顶叶下小叶、缘上回及豆状核有明显激活点，激活点主要集中在大脑皮层，深部激活点主要在壳核；4、非穴假针刺能显著激活BA6、8、9、10、18、21、37、40、43、44区等区域有激活点，主要分布在大脑的右侧，左侧也有不少激活点，就其具体分布主要在颞叶和额叶，少部分分布在顶叶和枕叶；5、外关穴真针刺与假针刺对比发现，BA8区和小脑等区域有明显的激活点；6、外关穴真针刺与非穴真针刺对比发现BA2区、左侧小脑等区域有激活点；7、外关穴假针刺与非穴假针刺对比发现，BA7、8、9、18等区有激活点，主要反应点在左侧大脑的枕叶中回、楔叶，顶叶楔前叶及额上回、中回，就其分布不难看出主要在大脑中部，颞叶未见明显激活点。

Results: The results showed that NOS positive neurons existed in all the auditory afferent nucleus while NOS negative neu-rons existed in the superior olivary nucleus. Most of NOS positive neurons in CNC were located in the posterior ventral cochlear nu-cleus ,which were round or oval bipolar neurons. NOS positive neurons were also found in potine widely.

结果：在脑桥的各级听觉传入核团，均有NOS阳性神经元，而上橄榄复合体无NOS阳性神经元；耳蜗核内NOS阳性神经元，主要集中在后腹侧核，为圆形或椭圆型双极神经元；下丘的臂内侧核NOS阳性神经元呈小的、圆形细胞。

Results: The Fos-like immunoreactive neurons distributed mainly in the spinal trigeminal subnucleus caudalisipsilaterally and medullary visceral zone (MVZ,including nucleus tractus solitarius,ventrolateral medulla and the reticular formation between the first two),ln the sections processed for double staining of Fos and TH immunohisto chemistry, numerous Fos-LI neurons showed also TH- like immunoreactivity in their cytoplasm(Fos-TH cells)in MVZ.

结果：Fos样免疫反应神经元主要分布在同侧三叉神经脊束核尾侧亚核、双侧延髓内脏带(孤束核、腹外侧区及二者之间的网状结构)。在抗Fos 和抗酪氨酸羟化酶的双重免疫染色切片上，见到延髓内脏带内许多Fos阳性神经元的胞浆同时呈TH样免疫反应(Fos-TH双重阳性细胞)。

Methods The total RNA was acquired from young rabbit particular cartilage, the BMP7 gene was inserted into pcDNA-3.1 to construct eukaryotic expression vector plasmid of pcDNA3.1-BMP7; the target gene-BMP7 was identified respectively by PCR analysis, restriction enounces analysis and nucleotide sequencing; the plasmid was transfected into adult rabbit knee articular chondrocytes, then the expression of BMP7 was detected by in situ hybridization, PCR and Western blotting.

从体外培养的幼兔膝关节软骨细胞中提取总RNA；按GenBank BMP7基因序列化学合成2条引物，采用RT-PCR方法得到BMP7基因；将BMP7基因片段插入到真核表达载体pcDNA 3.1中，构建pcDNA3.1-BMP7真核表达载体质粒；利用双酶切、PCR及核苷酸序列分析鉴定BMP7目的基因；将重组pcDNA3.1-BMP7真核表达载体质粒转染家兔关节软骨细胞，分别采用原位杂交、PCR、Western blotting法测定BMP-7表达。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。