双核型

The mating type gene is inherit decision factor of compatibility with the performance type,P.ostreatus belongs to sexual reproduction heterothallism,tetrapolarity,Namely the colony which is consist of single spore mycelium will self-sterility,If conjugate sex gene with all for incompatibility,then crossing can dicaryotization and form sporophore.

交配型基因是性亲和表现型遗传决定因子，平菇为有性生殖异宗配合四极性类型，即单孢形成的菌落自体不育，不同单核菌丝在配对基因皆为杂交结合时才能双核化，形成子实体。

The structures of spin quimtuplet state of {2}3+ and {2}5+ are more stable than those of spin singlet states of these complexes.

同时双核之间的咪唑桥环也对稳定该模拟物的构型起到了一定的作用。

Results Stable Spectral Karytyping method was set up and extraordinary multiplicity of structure, including metathesis、depletion、amplification、rearrangement、equiarm、marker-body and so on were found by this methad.

结果 我们建立了稳定的光谱核型分析方法，发现肿瘤实体瘤染色体异常呈多倍体且结构异常复杂，包括易位、缺失、扩增、重排、双着丝粒、等臂及标记染色体等。

The karyotype resemblance -near coefficients among the thirteen bivalves were computed according to the relative length and arm ratio of chromosomes. their average, mean square, extreme margin and standard deviation. The kindredship of thirteen bivalves were analyzed with the method of UPGMA.

方法］根据染色体相对长度、臂比，以及它们的平均值、方差、极差、标准差等参数，计算了13种双壳贝类之间的核型似近系数，用UPGMA法对它们的亲缘关系进行了聚类分析。

These findings convincingly confirmed that the isochromosomes 8q were monocentric chromosomes and that they were truly isochromosomes 8q rather than isodicentric chromosome (8)(p11) or a translocation between a chromosome 8 and any other chromosomes.

由此证实此核型为47，XY，-8，+i(8)(q10)×2，而非等臂双着丝粒8q或8号染色体与其它染色体易位所致。

The coordination geometry of the Eu atom is best described as a strongly distorted square-antipyrism.. The two Eu atoms are bridged by two N-oxide oxygen atoms forming a dimeric unit with crystallographic inversions centre. The Eu……Eu distance is 4.2137(9)〓. The two europium atoms and two bridging oxygen atoms is coplanar. The angles of Eu(1)-O(7)-Eu(1A) and O(7)-Eu(1)-O(7A) are 117.82(14)° and 62.18(14)°, respectively. 15 is the first dinuclear triboluminescent lanthanide complex reported.

同时还测定了其摩擦发光光谱与其光致发光光谱，并讨论了晶体结构和摩擦发光活性之间的关系。X-射线结构分析表明，15为有对称中心结构的配合物，每个Eu与三个HTTA的六个O原子和两个PyO的两个O原子形成八配位的四方反锥几何构型，2个N-Oxide O原子桥联两个Eu，形成了一个双核配合物，Eu。。。。

In this paper, the distribution of four subclasses of four subclasses of Seyfert Galaxy ( Seyfert 1s,Seyfert 1.5s, Seyfert 2s and Seyfert 3s or lin -ers)in the two -color diagram are comoared, It is found that the four subclasses have significantly different distribution in two -color diagram and have significantly different average color index, and especially Seyfert 1s are bluer than Seyfert 2s.

文章比较了四种赛弗特星系子类(1型赛弗特星系，1.5型赛弗特星系，2型赛弗特星系和3型赛弗特星系或低电离核星系)大双色图中的分布情况。结果发现四种赛弗特星系子类在双色图中具有显著不同的分布，特别地1型赛弗特星系比2型赛弗特星系要蓝。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

The genes of hammerhead ribozymes targeting against two and three sites on negative strand of potato spindle tuber viroid were designed, synthesized and cloned according to the action manner of hammerhead ribozyme.

根据锤头型核酶的作用模式，设计、合成并克隆了特异切割马铃薯纺锤形块茎类病毒负链RNA不同区域位点的双价和三价锤头型核酶基因。通过体外转录，将PSTVd负链RNA分别与双价和三价核酶混合，37℃温育 2h。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐