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The karyotypes and the Giemsa-C banding patterns of Aegilops tauschii (85A-34) and Hordium vulgare (H2) and regenerated plants from embryo callus of hybrids between Ae.tauschii and H.vulgare (2n=28) were confirmed by karyotype analyzing and Giemsa-C banding technique in this study, thus proved that the regenerated plants from embryo callus of hybrids between Ae.tauschii and H.vulgare (2n=28) were spontaneously chromosal doubling amphidiploid.

本研究通过染色体组型分析和Giemsa--C带显带技术,确定了节节麦(85A85A-34)、栽培大麦(H2)、节节麦×大麦杂种胚再生植株(2n=28)的染色体组型和Giemsa--C带核型,证明了节节麦×大麦杂种胚再生植株(2n=28)为自发加倍的节节麦-大麦双二倍体。

By Giemsa--C technique, the chromosomes of Aegilops tauschii (85A-34) and Hordeum vulgare (H2) and Ae.tauschii- H.vulgare amphidiploid were all produced clear bands, the terminal band, centromeric band, secondary constrition band and rich intercalary bands were displayed distinctly, thus the corresponding Giemsa-C banding patterns were confirmed.

利用Giemsa--C带显带技术,节节麦、大麦、节节麦-大麦双二倍体都显示出了清晰的带纹,都具有端带、着丝点带、随体带和丰富的中间带,相应的确定了它们的Giemsa--C带核型。

The synthesis of Zr-Zr bimetallic complexes was studied.

1对Zr-Zr型双核茂金属化合物的合成进行了进一步的尝试。

Furthermore, the structures of the complexes are strange. The tripodal ligand H〓L〓 form a 2: 2 ring-like binuclear complexes [Eu〓·L〓·6DMF] ·CH〓OH·DMF with Eu〓 in DMF. Two DMF molecules are accommodated in the ring and coordinate with Eu〓.

此外配合物的结构还比较特殊,三足配体H〓L〓与铕的配合物在配位能力较强的溶剂DMF中形成环状的2:2型双核配合物[Eu〓。L〓·6DMF]·CH〓OH·DMF,环状空腔刚好能够把两个配位的DMF溶剂分子包在里面。

Expression of hepatocyte markers such as ALB and CK18 was measured with immunohistochemistry. RESULTS: Cells in control group kept the long spindle shape, with shape agent as 4.5±1.0 and small size, proliferated quickly, submitted typical fibroblast whirlpool or flamboyancy growth. Five to seven branches were observed at the late culture period, no cell rounded, and the rate of dikaryocyte was less than 0.1%.

结果:未进行诱导的对照组细胞保持长梭型,形状因子4.5±1.0,面积较小,增生较快,呈典型成纤维细胞的漩涡状或火焰状生长,培养后期可见5~7个分支,无细胞变圆,双核细胞率小于0.1%,逐渐老化,颗粒增多。

The synthesized cDNA is a continuous band less than 6. 0kb shown on x-film through α〓PdCTP which was inserted in the first strain of cDNA. The titer of the Ha-cDNA library was about 1. 5×10〓cfu/ml which could represent all protein-encoding sequences from Hz-AM1 cells; Namely, the cDNA library has representativity. After the successful construction of the Ha-cDNA library, the open-reading frame of vp39 gene of Heliothis armigera was then fused in frame with the Ga14-DNA-binding domain in the pGBT9 plasmid and the fusion protein Ga14-VP39 was expressed in yeast to be used as bait protein.

在成功构建棉铃虫细胞Hz-AM1cDNA文库的基础上,本文接着将棉铃虫核型多角体病毒衣壳蛋白VP39(HaNPV-VP39)基因克隆到酵母双杂交系统(yeasttwo-hybrid system)中的质粒pGBT9上。pGBT9上含有酵母菌转录激活因子GAL4 DNA结合域的编码区(GAL4-DBD),使克隆到质粒pGBT9上的HaNPV-vp39基因与GAL4-DBD基因融合,在酵母菌中表达DBD-vp39融合蛋白,该融合蛋白即为酵母双杂交系统中的诱饵蛋白。

Results The frequencies of micronucleated binucleated cell in 30 nmol/L FA were significantly higher than those in 60, 120, 240, 2 260 nmol/L FA (P.001~0.05), but no significant differences were observed either between those in 60 and 120 nmol/L FA, or between those in 120, 240 and 2 260 nmol/L FA within either groups when the genotype was the same.

结果 MTHFR C677T三种基因型的乳腺癌个体与对照组淋巴细胞在叶酸浓度为30 nmol/L时双核细胞微核率均显著高于60、120、240和2 260 nmol/L测试组(P.001~0.05),60 和120 nmol/L 两个测试组之间以及120、240和2 260 nmol/L三个测试组之间都未发现显著性差异。

In tetrapolarity mating system,A and B factor is two mating types locus locating different chromosome,only when two single spore mycelium form AαAβBαBβ,then can produce to dikaryon and complete whole sexual life history.Study according to the Eger.

在四极性交配系统中,A和B因子分别对应位于不同染色体上的两个交配型位点,两个单核菌丝相遇,只有当它们形成A1A2B1B2或者AαAβBαBβ型时,才能产生具有锁状联合的双核体,完成整个有性生活史。

Under nitrogen limitation conditions, cells of opposite mating type, MATa and MATα, fuse to form dikaryotic filaments, and subsequently produce the meiotic basidiospores. Our prior studies have shown that C.

在一般培养状态下,主要以酵母菌的型态存在;於氮素源缺乏的环境下,两种交配型的细胞会进行细胞融合,产生双核菌丝,最终形成担子孢子。

The vascular bundle of stem is the type of double phloem. The anther is tetrasporangiate. Simultaneous cytokinesis in the microspore mother cell follows meriosis.

茎的维管束是双韧类型;雄蕊的花药四室,小孢子的形成为同时型胞质分裂,悬垂胎座,倒生胚珠,核型胚乳,果实成熟时胚仍处于球形胚阶段。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。