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The karyotypes and the Giemsa-C banding patterns of Aegilops tauschii (85A-34) and Hordium vulgare (H2) and regenerated plants from embryo callus of hybrids between Ae.tauschii and H.vulgare (2n=28) were confirmed by karyotype analyzing and Giemsa-C banding technique in this study, thus proved that the regenerated plants from embryo callus of hybrids between Ae.tauschii and H.vulgare (2n=28) were spontaneously chromosal doubling amphidiploid.

本研究通过染色体组型分析和Giemsa--C带显带技术,确定了节节麦(85A85A-34)、栽培大麦(H2)、节节麦×大麦杂种胚再生植株(2n=28)的染色体组型和Giemsa--C带核型,证明了节节麦×大麦杂种胚再生植株(2n=28)为自发加倍的节节麦-大麦双二倍体。

Cathepsin D and β-tubulin marked with fluorescence were followed in distribution observed by laser scaning microscope, the distribution was from nucleus nearby to cytoplasm and apophysis, which suggested that they took part in traffic in cells.

荧光双标组织蛋白酶D及β-tubulin,共焦激光扫描显微镜下观察,可见二者伴随分布,均由开始的核周分布明显到散在分布于胞质及突起中,说明溶酶体与骨架蛋白密切相关,提示共同参与细胞内的运输。

The result of framework protein and lysosome marked with fluorescence was observed in sym-acorched laser scaning microscope: red fluorescent β-tubulin and green fluorescent eathepsin in nerve cell were seen in distribution in the same sample. The distribution of both was the same, and was located in around nucleus in the early of differentiation, and in cytoplasm and apophysis in the maturity of differentiation.

共焦激光扫描显微镜镜观察荧光双标的骨架蛋白和溶酶体的染色结果:在同一标本上,不同波长的单光激发可分别见到发红色荧光的β-tubulin和发绿色荧光的组织蛋白酶D在神经元内的存在及分布情况,二者的分布区域大致相同,神经元分化初期,较集中分布在核周附近,神经元分化成熟则分布于胞质及突起内。

Numerical results of single solid/core-shell and two cylindrical nanorods under plane electromagnetic wave with resonance frequency are presented.

本研究主要探讨不同细长比之单、双颗实心或核-壳细杆形金奈米粒子於电、磁场中受到平面波或电偶极波源时,散射体的表面电浆子现象。

AIM: To study the function of hepatitis C virus internal ribosome entry site, and to construct biscistronic vector.

目的: 研究丙型肝炎病毒核糖体插入位点启动翻译蛋白质功能,构建双顺反子真核表达载体。

Series of octahedral core-shell emulsion microcrystal with different distribution of iodide ions was prepared by double-jet method.

本文应用双注法制备了一系列碘分布不同的八面体核壳乳剂。

By using PCR method, a 1kb fragment was gained from Heliothis amigera nuclear polyhedrosis virus genome.

用PCR方法从棉铃虫核多角体病毒基因组中扩增到了1kb的DNA片段,用双脱氧链终止银染测序法测定了该片段的全序列,发现它含有一个完整。。。

Fluorescence in situ hybridization was carried out to confirm the integration of HBV DNA into male pronucleus and its replication with cell division in embryonic development.(Reverse transcriptase polymerase chain reaction,RT-PCR) and immunofluoresence assay were performed to observe the expression of the HBV gene in two-cell stage.

材料与方法:成熟雄鼠麻醉后双侧睾丸注射经脂质体DOSPER包裹的HBV质粒,手术后雄鼠与超排雌鼠合笼交配,用荧光原位杂变(Fluorescence in situ hybridization,FISH)分别检测单细胞胚和二细胞胚间期核中HBV DNA的存在与复制,用逆转录聚含酶链反应(Reverse transcriptase-polymerase chain reaction,RT-PCR)和免疫荧光方法检测HBV基因在二细胞胚胎中的表达。

Infected oligodendrocytes or type 2 PML cells with oval enlarged showing replacement of granular chromatin by basophilic or amphophilic, homogenized, ground-glass like viral inclusion bodies different from eosinophilic viral inclusion bodies of Cowdry type

感染的少突胶质细胞,或2型PML细胞,伴卵圆形拉长的核,显示颗粒状染色质变成嗜碱性或双嗜性、均质、毛玻璃样病毒包涵体(与Cowdry型的嗜酸性病毒包涵体不同)。

Chiral SalanCrX complexesa saturated version of SalenCr(ⅢX are designed,and in conjunction with an ionic quaternary ammonium salt or organic strong base can efficiently catalyze the asymmetric,regio-and stereoselective alternating copolymerization of CO_2 with rac-PO at mild conditions to afford isotactic-enriched polycarbonates with~95%head-to-tail linkages and moderate enantioselectivity.

本论文在Salen构型配体的基础上,设计合成了一系列手性Salan型铬配合物,并将其与季铵盐/有机强碱组成亲电-亲核双组分催化体系,在室温下可以有效的催化CO_2和环氧丙烷的不对称交替共聚反应,实现了CO_2和环氧丙烷的不对称交替共聚反应的区域和立体化学控制,得到95%的头尾连接单元和中等光学纯度(对映体过量值ee%≈70%)的全同结构的聚碳酸酯。

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