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Centromeres could be identified easily on the bivalents at diplotene, so it was convenient to analyse the karyotype.

结果表明,日本沼虾染色体数n=52,2n=104,据双线期二价体的相对长度、着丝点位置以及形态诸方面的特点分析了核型,共分为A,B,C,D4个染色体组,核型组成是N=37M+4ST+11T;初级精母细胞减数分裂前期I可分为细线期/偶线期、粗线期、双线期和终变期5个时期,双线期的二价体存在弥散阶段,核仁明显,且双线期二价体着丝点清晰,给核型分析带来了方便。

While under natural low temperature (10-20℃), a great deal of abnormalities, including micro nucleoli, scattered and lagged chromosomes and anaphase bridges were detected during PMC meiosis, even though the abnormalities were different between the two cultivars; the ratio of micro nucleoli was 22.88% of average, which was obviously higher than that of control (4.56% of average under normal temperature); and the ratios of univalent and multivalent in diplotene, which increased seriously, were 8.82% and 17.65% of Tainong 1, and 14.52% and 24.19% of Irwin under natural low temperatures.

低温(10~20℃)条件下,减数分裂过程中核仁的行为发生大量异常,微核仁的平均检出率高达22.88%,明显高于常温条件下的平均4.56%;双线期单价体和多价体的发生频率有明显增加,其中台农1号为8.82%和17.65%,Irwin为14.52%和24.19%;减数分裂中期Ⅰ、Ⅱ及后期Ⅰ、Ⅱ均观察到落后染色体,其中台农1号异常率为12.73%、5.63%、15.79%和4.76%,Irwin为11.11%、7.61%、9.52和6.38%。

In the treatment of higher concentration of NaN_3,some cells appeared crystalloid,two nuclei,abnormal nucleus,nucleolus losing.

较高浓度NaN3处理的PLB细胞中出现晶体物质、双核、畸形核和核仁丢失等现象。

The ultrastructure characters of pollen grain in Brassica napus are:1There are vacuoles in cytoplasm but no starch grain in uniceelluar pollengrain.2The veget- ative nucleus of bicellular pollen grain is generally spheroidal;while the generative cell is spinde-shaped,with a big nucleus,thin layer of cytoplasmand few cell orgens,no cell wall.3In three-celled pollen grain the sperm cell is separated from the cytoplasm of vegetative cell by two layers of plasmic membrances.

Brassica napus的花粉粒,在不同发育时期超微结构的特征如下:1单胞花粉粒有一个球形的细胞核和明显的核仁,细胞质内出现液泡,缺少典型的淀粉粒。2双胞花粉粒的营养核多为球形。生殖细胞纺锤形,无细胞壁,细胞核比例大,细胞质呈薄层,细胞器少。3三胞花粉粒有一个裂片状的营养核和两个纺锤形的精细胞,精细胞无壁,以两层质膜与营养细胞的细胞质相隔。

RESULTS: After 24 hours of preinduction, cells changed from spindle to polygonal or irregular, with many short rod-shape processes and 2-3 nucleoli. Whirlpool shape growth disappeared, and was disordered. After 1-3 hours of induction, cells gradually became round, with significant cell contraction, strong refraction, bipolar or pluripolar processes connected each other into a net. After 5 hours of induction, grades one and two branches were detected.

结果:预诱导24 h,胞体由原来的长梭形变成多角形或不规则形,伸出多个短棒状突起,可见2~3个核仁,细胞间漩涡状生长趋势消失,排列无规律;诱导1~3 h,细胞逐渐变圆,胞质收缩明显,折光性增强,双级或多极的突起互相连接呈网状;诱导5 h,突起出现一、二级分支。

Furthermore, by using two-colored probes to study the relationships between RNA polymerase Ⅲ and RNA polymerase I, we observed that the signals of the two RNA polymerase were very close.

同时,应用这一研究方法对RNA聚合酶Ⅰ的转录发生在核仁中的结论进行了确证,并进而应用双探针标记荧光原位杂交的方法对RNA聚合酶Ⅲ和RNA聚合酶Ⅰ转录位点的关系进行了初步探讨,结果显示两者的转录位点之间非常接近,并且有伴随发生的趋势。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。