双染的

RESULTSdiabetic rats showed typical symptoms involving elevated serum glucose and weight loss (2)The rats in DM+VaD group reacted slower and made more error numbers in Y-maze than the rats in VaD group did, with a prolonged total reacting time in a whole day.(3)More neurons with serious damages, such as edema, ischemia or pyknosis could be found in DM+VaD group than in VaD group. More disarrangement of cone neurons, obvious glia proliferation, and more neuron apoptosis were found in CA area of hippocampus. CONCLUSIONS (1)2-VO in STZ-induced diabetic rats succeeded in establishing VaD animal models.(2) The cognitive dysfunction induced by VaD rats had been deteriorated by diabetes mellitus.(3)Morphology evidences proved that diabetes mellitus aggravated brain damages induced by VaD.PART II Effect of diabetes mellitus on the cholinergic nervous systemin CA1 area of hippocampus of VaDOBJECTIVE To estimate the role of cholinergic nervous system in hippocampus during the process of cognitive dysfunction aggravated by diabetes mellitus in DM+VaD group. METHODS Observe the changes of ChAT protein by immunohistochemistry stain. Chose three rats randomly from each group at each pointat two week, four week, and eight week, then sepa

经腹腔注射链脲佐菌素诱导慢性实验性糖尿病，1周后永久性结扎双侧颈总动脉(2-VO)制作VaD模型记录术后2周、4周和8周各组大鼠的体重及血糖；应用Y-迷宫检测空间定向学第二军医大学博士论文中…丈摘…要1ia；一色恤~﹁染澎糕行HE染色观察组织病理学孪叱；胶质细胞原纤维酸性蛋白protellZ，Cf冰尸标记观察星形胶质细舱的激活情况、橄声软公点娜育票票众糯黑橄居端粗默孺篇筑价井袱第二部分糖尿病对血管性痴呆大鼠海马c心区胆碱能神经系统的影响探讨海马CAI区胆碱能神经系统在糖尿病加重确D一大鼠认知障碍中所实验动物及分组同第一部分，应用免疫组织化学染色方法现察海马CAI{介狱牛第晕军l袭拔常博粗{论义

RESULTS: Islets isolated and digested were stained red by dithizone and no stain was observed in the islet exocrine gland. The purity of islets was ≥ 85%. The results of MTT and functional detection showed that the survival rate of islets in the scaffold group was significantly higher than control group (P 0.05). The observation under scanning electron microscopy demonstrated that islets tightly adhered to the PGA scaffolds and grew in the three-dimensional status.

结果：分离和消化后的胰岛被双硫腙染成红色，胰岛外分泌腺不着色，胰岛纯度≥85%；MTT及胰岛功能检测结果显示，支架组的胰岛成活率及胰岛功能明显增高，与对照组相比差异有显著性意义(P 0.05)；扫描电镜观察，胰岛紧密黏附在聚乙醇酸支架上，支架上胰岛成三维立体生长。

By using PCR method, a 1kb fragment was gained from Heliothis amigera nuclear polyhedrosis virus genome.

用PCR方法从棉铃虫核多角体病毒基因组中扩增到了1kb的DNA片段，用双脱氧链终止银染测序法测定了该片段的全序列，发现它含有一个完整。。。

Suspects of DS and 26 cases of fetal amniocytes come from pregnant woman with high risk of DS were undertook to analysis 10 STR loci simultaneously by PAGE and silver staining or automated fluorescent laser activated detector sequencer (ABI310). 6 suspects of DS were determined as DS by trisomic triallelic with a ratio of 1: 1: 1 and the other STR loci show nonnal diallelic pattern with a ratio of 1: 1 or homozygous with two alleles of the same size. The other one suspect determined as DS by 7 STR marker showing trisomic diallelic band or peak.

将10个STR位点的PCR产物用聚丙烯铣胺凝胶电泳和银染法或基因片段扫描法进行检测，结果从23例可疑患者中发现7例DS患者，其中6例均出现三条带图谱，1例虽未发现3条带图谱，但除纯合子外其余位点均表现为双倍剂量（2：1）的2条带图谱。26例胎儿羊水细胞STR基因型均表现为正常2条带图谱（1：1），表明为非DS胎儿。

Methods: The olfactory nerve layer and glomerular layer was isolated from the olfactory bulb of three-day old rats, Enzyme digestion method was used to isolate cells and the cells were purified by differential adhesion method. Freshly isolated OECs were plated onto poly-L-lysine-coated tissue culture and then incubated for 2 days. The purity of OECs was identified by immunocytochemistry of NGFRp75 and S100 and Hoechst33342 counterstain method.

从新生SD大鼠(3d)嗅球中迅速分离嗅神经层和嗅颗粒层，采用酶消化法分离细胞，差速贴壁法纯化细胞，接种于多聚赖氨酸包被的培养板内培养2d，采用NGFR p75和S100蛋白双标免疫组化、以Hoechst33342复染鉴定OECs的纯度。

The key point of production of cotton double warp fabric is the selection of pigment for printing so as to reach the satisfied color fastness of sunlight and dry cleaning resistant Process recipe and condition is given in the paper .

通过小试和中试，制订了纯棉双经装饰用布的染整工艺，认为其生产关键是优选印花涂料，以达到令客户满意的耐日晒和耐干洗牢度。

Heat-killed bifidobacterium has curing effect to mice with enterogenic infection caused by immunocompromise same as live bifidobacterium.

双歧杆菌灭活菌液具有与活菌液相同的抗免疫功能低下致小鼠肠源性感染作用。

There is a blue print cloth or double-sided, which need to be positive siningensis strickle pulp making copy table on reverse alignment positive patterns and then strickle pulp and yeum before you can get a printed double-sided blue.

有的蓝印花布还是双面的，这就需要在正面刮浆干透后，利用拷贝桌在反面对准正面纹样再刮浆一次，这样染后就可得到双面的蓝印花布。

During processing of polyester and polyamide yarns to manufacture wovens and knitgoods piece-dyed bico-lour effects such as white resists, ombre and differential dyeing as well as tone-in-tone dyeing can be reached.

聚酯/酰胺交织机织物或针织物的匹染过程中，可以实现诸如防白染色、虹染色、异染色及同色深浅效应染色等的双色效果。

A process for preparing the recombinant chicken gamma-interferon (rCHIFN-gamma) with high activirus activity includes such steps as cloning ChIFN-gamma gene from eukaryotic plasmid to transfer carrier by dual enzyme servenings to obtain recombinant transfer plasmid pFASTBACI-ChIFN-gamma, taking DH10Bac competent cell, adding IngpFASTBAC1-ChIFN-gamma, transposition, diluting to culture object by SOD culture medium, coating on Luria plate, culturing, choosing white clony, purifying, naming positive plasmid as Bacmid-ChIFN-gamma, extracting its DNA, and transfecting Sf9 cells.

高抗病毒活性重组鸡γ-干扰素的制取方法及用途，涉及一种广谱高效抗病毒基因工程产品的制取方法。将ChIFN-γ基因从真核质粒中经双酶切后克隆到转移载体中，获得重组转移质粒pFASTBAC1-ChIFN-γ；取DH10Bac感受态细胞，加入1ng pFASTBAC1-ChIFN-γ，转座后，用SOC培养基稀释培养物，涂布Luria平板，培养后，挑取白色菌落，Luria平板进行纯化，阳性质粒命名为Bacmid-ChIFN-γ，提取阳性质粒DNA；取上述重组DNA质粒转染Sf9细胞，制得高抗病毒活性重组鸡γ-干扰素。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新