双染的

Western blot and double staining analyses show NYD-SP27 becomes detached from sperm, as they undergo capacitation and acrosome reaction.

Western blot和双染的研究表明在精子发生获能和顶体反应的过程中NYD-SP27逐渐从精子上丢失。

E and TUNEL Besides,early apoptotic cells which were densely green yellowstained or displaying green yellow fragments,late apoptotic cells which were densely cardinal redstained or displaying cardinal red fragments, and cardinal red swollen necrotic cells could be found in the AO/EB doubly stained cells.

AO/EB双染色法还能够显示呈致密浓染的黄绿色染色或出现黄绿色碎片的早期凋亡细胞，呈致密浓染的鲜红色染色或出现鲜红色碎片的晚期凋亡细胞，以及呈鲜红色膨大状的坏死细胞。

Through discussions on the effect of shrinkage in scouring-dyeing on the apparent and inherent quality of crepe de chine products, suggestions are made on some adjustments of crepe de chine's specifications and shrinkage in scouring, i.

探讨了染整工艺中练缩率对双绉成品外观质量和内在质量的影响，建议对双绉的规格和练缩率进行适当调查，将"统一出口绸缎规格"(1983年编)中规定的双绉经向练缩率2％～4％提高到8％～11％，以提高真丝双绉的成品质量。

Flow cytometry was used to measure the cells cycle kinetics of the treated cells. Hoechst33258/PI double staining was performed to evaluate the cell morphology. Western blotting was conducted to analyze the expressions of p53, Bcl 2 and Bax. Results 2 ClAdo and 2 CldAdo had different cytotoxicities on the cells, while 2' dAdo had no cytotoxicity on the cells.

以胎盼蓝活细胞拒染法测定药物对细胞生长、增殖的抑制及IC50 ；以流式细胞术观察药物对细胞周期的影响；以荧光双染法（Hoechst33258/PI）观察药物对细胞形态学的影响；以Western blot法观察药物对细胞P53、Bcl 2、Bax蛋白表达的影响。

Applied rabbit anti-5-HT and mouse monoclonal anti-TPH antisera, the localization of 5-HT and its synthesizing enzyme TPH in the visual system, midbrain, and suboesophageal ganglion of the beetle Harmonia axyridis (Coleoptera: Coccinellidae) was studied by Streptavidin-Peroxidase immunohistochemical method on Colophony-Paraffin embedded serial sections.

运用树脂石蜡组织包埋切片技术，结合免疫组化链霉菌抗生物素蛋白-过氧化物酶双染法，采用兔抗5-HT抗血清和鼠抗TPH单克隆抗体，初步构建了异色瓢虫Harmonia axyridis视觉系统、脑中部及咽下神经节5-HT及其合成酶TPH抗原系统的免疫组织化学反应模式。

Methods SECs were isolated by collagenase perfusion combined with isopycnic centrifugation in a two-step percoll gradient and selective adherence, and identified by morphologic observation under light microscope, by immunocytochemistry for CD14 and Ⅷfactor related antigen, by immunofluorescence staining for RECA-1 and fenestrations under SEM.

方法用胶原酶灌注结合Percoll密度梯度离心加选择性贴壁的方法分离SEC；用光镜形态学、Ⅷ因子和CDl4免疫细胞化学染色及RECA.1单抗间接免疫荧光法及SEM鉴定SEC；采用TEM及AnnexinV联合PI双染的方法检测SEC的凋亡。

⑴There are NOS/ER positive neurons in the hypothalamic paraventricular nucleus, supraoptic nucleus, periventricular nucleus and lateral hypothalamic area.⑵The double-labeled neurons were mostly distributed in the medial dorsal and lateral dorsal of supraoptic nucleus and in the medial parvicellular, ventral zone. They were extensive slightly,but scattered in lateral hypothalamic area, sporadic in the periventricular nucleus.

1在大鼠下丘脑视上核、室旁核、下丘脑外侧区及室周核均有NOS与ER双染神经元分布。2 NOS与ER双染神经元主要集中分布在视上核的背内侧和背外侧部及室旁核小细胞部腹内侧区，在下丘脑外侧区分布较广但比较分散，室周核呈散在分布。

Sulfonic-4-(β-sulphatoethyl-sulphone) phenylamine is often applied to producing bifunctional-soluble reactive dyes and is an important dye intermediate. It plays an important role in the synthesis of KN style reactive dye M style double reactive dye and modified dissimilar double reactive dye. Used 2-sulfonic-4-(β-sulphatoethyl-sulphone) phenylamine as raw material can improve the uniformly dying performance and scourablility of dyestuff molecules and can improve the firm degree of colour staining.

2-磺酸-4-苯胺是制备水溶性双活性染料的重要中间体，在KN型活性染料、M型双活性染料以及改性异种双活性染料的合成中起着重要的作用，用2-磺酸-4-苯胺作原料合成活性染料可增加染料分子的匀染性和易洗涤性，提高染料的粘色牢度。

Of CBMC can be purified by Mini-MACS as CD34〓 stem cells. B. The number of CD34〓 stem cells can expand to 40.24±9. 86 fold after 14 days. C. No matter in the expression of CD1a, CD80, CD86, and HLA-DR, or in the function of stimulating xenogenous lymphocyte proliferation, there was no difference between CD34+ stem cell DCs or monocyte DCs. D. The percentage of CD3〓CD56〓 cells is the same in CIK cells co-culture with DCs transfected with SKOV3 RNA, CIK cells co-culture with DCs, and CIK cells. E. The expansion rate of CIK cells can be accelerated by co-culturing with loaded or unloaded DCs. However, the expansion rate between loaded or unloaded group is the same. F. The strongest cytotoxicity against SKOV3 cell line was achieved by CIK cells co-cultured with DCs loaded with SKOV3 RNA.

结果：1、Mini-MACS分选系统可自CBMC中提取0.78±0.31%的CD34〓细胞；2、体外培养14天后可获得原始CD34〓细胞量40.24±9.86倍的细胞；3、不论在CD1a、CD80、CD86及HLA-DR的表达上，或是刺激异体淋巴细胞增生的功能上，脐带血CD34〓细胞与单核细胞来源的DC都没有差别；4、CIK细胞中CD3〓CD56〓双阳性的表达率在与RNA转染DC共培养的CIK细胞组、与DC共培养的CIK细胞组及单纯CIK细胞组3组间比较无差异；5、脐带血CIK细胞增殖显著，培养14天时可扩增18.18±5.59倍，培养21天时可扩增35.02±6.30倍；5、与未转染或转染DC共培养的CIK细胞在培养第14天后增殖速率大于单纯CIK细胞。

Through discussions on the effect of shrinkage in scouring-dyeing on the apparent and inherent quality of crepe de chine products, suggestions are made on some adjustments of crepe de chine's specifications and shrinkage in scouring, i.

探讨了染整工艺中练缩率对双绉成品外观质量和内在质量的影响，建议对双绉的规格和练缩率进行适当调查，将&统一出口绸缎规格&(1983年编)中规定的双绉经向练缩率2％～4％提高到8％～11％，以提高真丝双绉的成品质量。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。