双杆菌

Results:The level of total anaerobic population and total aerobic population of excrement specimens of three kinds of IBS sufferers were different significantly, particularly the level of bifidobacterium and cillobacterium(P.01);the level of bacteroid population was higher significantly in diarrhea-predominant and mixed type of IBS sufferers (P.01);the level of enterococcus population was higher significantly in diarrhea-predominant IBS sufferers(P.05);among the aerobes, the number of Escherichia coli and Staphylococcus aureus colonies were increased by different level(P.01or0.05) in the IBS suffers, in particular, the number of Escherichia coli colonies in diarrhea-predominant IBS sufferers increased most significantly.

结果：三种类型的IBS患者粪便中总厌氧菌群和需氧菌群数目与健康人差异明显，特别是双歧杆菌属和乳酸杆菌属明显减少（P.01；拟杆菌菌落数在腹泻型和混合型IBS患者中明显增加（P.01）；肠球菌菌落数在腹泻型IBS患者中明显增加（P.05）；需氧菌中患者组大肠杆菌和金黄色葡萄球菌菌落数均不同程度增加（P.01或0.05），大肠杆菌菌落数在腹泻型IBS患者中增加最明显。

Project Content: Plan to utilize the local plentiful carrot resources and adopt the diplococcus fermented technology to build one diplococcus fermented carrot compound beverage production ling with the annual production capacity of 2000 tons.

项目内容：该项目拟利用当地丰富的优质胡萝卜资源，采用双歧杆菌发酵技术，建设年产2000吨双歧杆菌发酵胡萝卜复合汁生产线一条。

We also compare the correlation between normal PCR bifidobacteria quantification and traditional diluting bifidobacteria quantification.

并且比较普通PCR定量的双歧杆菌结果和传统稀释滴种法定量的双歧杆菌结果的相关性。

The test of white blood cell count was finished by the different number of bifidobacterium suspension coloclysis and general irradiation with 5.0 Gy γ ray to establish acute radiation damage pattern.The 30 survival rate was tested and reported after the bifidobacterium suspension coloclysis of 1×109CFU/ml and 5.0 Gy ray to establish acute radiation damage pattern number in every day.

WBC计数：用不同数量级浓度的双歧杆菌菌悬液对小鼠灌肠28 d后，5.0 Gy照射后3 d、6 d和10 d采取小鼠外周血检测WBC数。30 d小鼠生存率：用1×109CFU/m l双歧杆菌菌悬液灌肠28 d后，用一次性8.0 Gy60Coγ射线全身照射，建立急性放射损伤模型，每日上下午各观察小鼠1次，记录死亡动物数和死亡日期。

The levels of SOD and CAT significantly increased and MDA decreased in bifidobacterium group compared to radition damage group.Electronic microgragh of spine neurocyte and neurogliocyte showed that cytoplasm,cytoblast and plastiosome were significantly engorged ,and neurogliocyte apoptosised in earlier period. TLR2mRNA increased in C group compared to B group by RT-PCR. Cytochrome C in kidney increased in C group compared to B group, but bcl-2,NF-Кb decreased. Conclusions : Enteroclysm is better compared to intragastric administration.

双歧杆菌处理组肝组织中SOD和CAT水平明显高于放射损伤组(P.01)，同时MDA明显低于放射损伤组(P.01)；电镜结果显示：放射损伤组与双歧处理组的细胞浆、细胞核和线粒体均明显肿胀，双歧处理组同时具有早期凋亡现象；RT-PCR结果显示：双歧处理组TLR2mRNA的表达明显高于放射损伤组；免疫组化结果显示，双歧处理组Cytochrome C的表达明显优于放射损伤组，而NF-кBp65和bcl-2则相反。

On 4th, 11th and 18th experiment day, intestinal bacteria, i. e. enteric bacilli, enteric enterococci, Bacillus bifidus, and Bacillus acidi lactici, on colonic and ileac mucosa of the mice were analized quantitatively and their content in liver, spleen, kidney and blood was determined.

在实验第4、11及18天，对各组小鼠结肠与回肠肠黏膜肠杆菌、肠球菌、双歧杆菌、乳酸杆菌进行定量分析，并取小鼠肝脏、脾脏、肾脏、血液做细菌培养。

Secondly, Bifidobacterium medium was developed using JAJ as main material. The results showed that: Adding Tryptone,soy peptone.beef extract,com extract and Cys-HCl to JAJ could obviously promote the growth of Blm and Bbm;By the orthogonal experiment of three elements and three levels,a satisfying JAJ compound medium was acquired which included corn extract(0.3%), soy peptone(0.05%)and Cys-HCl(0.025%).Nextly,after establishing a selective Bifidobacterium medium, the effects of JAJ on the growth of Bifidobacteria in vivo were studied,using healthy mouse of Kunming species as experimental animal.

研究了以菊芋汁为主要原料的双歧杆菌培养基，大量试验结果表明，在菊芋汁中添加胰蛋白胨、牛肉膏、大豆蛋白胨、玉米浆和半胱氨酸盐酸盐等成分，对双歧杆菌有明显的促进生长作用；利用大豆蛋白胨、玉米浆和半胱氨酸盐酸盐设计了三因素三水平的正交试验，确定了菊芋汁复合培养基的优化配方：菊芋汁+0.3％玉米浆+0.05％大豆蛋白胨+0.025％半胱氨酸盐酸盐。

Put the detrained Bifidobactirium in different consistency berberine-culture medium and keep on screen Bifidobactirium that proof berberine.

将分离出来的双歧杆菌在不同浓度的黄连素培养基上培养，不断筛选获得抗黄连素的双歧杆菌。

The translocated bacteria and endotoxin trigger the production of inflammatory mediators, activation of complement and coagulation system.

纠正SIRS患者胃肠电活动的异常③保护肠道双歧杆菌和乳杆菌，抑制肠杆菌等革兰氏阴性细菌过度生长。

The study also found that the predominant intestinal florae of Giant panda returned to wild and Giant panda in captivity were enterobacteria, enterococcus and lactobacillus.

酵母菌、乳杆菌、类杆菌、双歧杆菌，小韦荣氏球菌，空肠弯曲杆菌。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐