双杆菌

Results The ISR sequence of Bifidobacterium longum containing 568 bp,was the same as Bifidobacterium breve and they were different from Bifidobacterium adolescentis and Bifidobacterium infantis which were only 510 bp.

结果长双歧杆菌和短双歧杆菌16S~23S rDNA ISR序列一致，全长568个碱基对；青春双歧杆菌与婴儿双歧杆菌序列一致，全长510个碱基对。

This paper studies the effects of Jerusalem artichoke on the growth of Bifidobacterium in skim milk in order to provide theoritical foundation for the utilization of Jerusalem artichoke resource and development of bifidus yogurt, using Jerusalem artichoke powder as experimental material and B.

以菊芋粉为材料，以长双歧杆菌和两歧双歧杆菌为实验菌株，研究菊芋在脱脂乳中对双歧杆菌生长的影响，以期为进一步开发菊芋资源和研制双歧杆菌酸奶提供理论依据。

The optimum hydrolytic conditions of razor clam for neutral protease were : enzyme concentration 2.4%, hydrolysis temperature 50癈, substrate concentration 1:3, pH 7 and reaction time 6 hrs with the degree of hydrolysis and extraction rate of total nitrogen 42.46% and 83.04% respectively; and foracid protease they were: en/yme concentration 6.0%, hydrolysis temperature 50癈, substrate concentration 1:4, pH3.5 and reaction time 5 hrs with the degree of hydrolysis and extraction rate of total nitrogen 46.37% and 87.94% respectively, the hydrolysate being clear light yellow. For the combination of two kinds of proteases they were: first hydrolyzing with 2.6% f 1 avouryme for 3 hrs, then hydrolyzing with 2.4% neutral protease for 3 hrs with the degree of hydrolysis and extraction rtiie of total nitrogen 4-1.57% and 86.95% respectively.

最后综合考虑了水解率、总氮回收率和水解液的色泽、澄清度等指标，得出了较适宜的酶种为单酶以枯草杆菌中性蛋白酶和酸性蛋白酶效果较好，双酶以风味蛋白酶与枯草杆菌中性蛋白酶双酶组合水解效果较好，其适宜的水解工艺条件分别为：枯草杆菌中性蛋白酶加酶量为2.4%，水解温度50℃，料水比1：3，pH值中性条件下水解6h，绕蛙蛋白质的水解率和总氮回收率分别为42.46%和83.04%；酸性蛋白酶水解编蛙肉的适宜的工艺条件为加酶量6.0%、水解温度50℃，料水比l：4，pH值3.5条件下水解sh，水解率为46.37%，总氮回收率为87.94%，水解液呈较清澈的淡黄色；双酶采用先用2.6%风味酶水解3h后，再加入2.4%中性蛋白酶水解3h，水解率达44.57%，总氮回收率为86.95%。

Firstly, normal mice was given orally by SM(qd×2d), and the dysbacteriosis were caused in them ,and then they were given orally by STM,these mice were formed experimental salmonellosis models ,finally these salmonellosis models were treated by bifidobacterium ,and count respectively the cfu/g of STM number in the mice excrement during different time of therapy,detect the index numbers of liver and spleen respectively, detect content of TNF-a in blood and in spleen respectively.

先用硫酸链霉素给正常小鼠灌胃2d，出现菌群失调症状，再用STM灌胃造成小鼠STM感染，使用两岐双岐杆菌液干预治疗STM感染小鼠，检测不同治疗时间各组小鼠粪便STM菌落数、肝脾指数、血及脾中TNF-a的含量。采用SPSS12.0统计软件包进行数据处理分析，统计学方法采用双因素重复测量资料的方差分析、单因素随机化设计的方差分析、卡方检验、多组均数比较的非参数秩和检验。结果：随着治疗天数的增加，STM菌落数逐渐减少，未干预组、生理盐水组及双歧杆菌组比较结果均为P＜0.01；组间比较，双歧杆菌组STM数下降最快。

And unrooted trees were established. The accession numbers in GenBank of the sequences of 16S rDNA of the strains were followed by PFK1(DQ295034), PFK2(DQ295035), PFK3(DQ295036), LZCL(DQ295040), ZCS1(DQ295037), MMA1(DQ295041), MMA2(DQ295038), JSQ1(DQ295042), JSQ2(DQ295039), JSQ3(DQ295043). The Blast and phylogenetic tree analysis showed that

3将分离菌株进行形态、生理和糖发酵实验，结果表明这些表型鉴定的结论与16 S rRNA基因序列同源性分析结论一致，分离纯化5种产品的10株菌中仍有相同的3株菌与标签标注的不一致，即其中标签上分别标注为青春双歧杆菌和长双歧杆菌的两株菌鉴定结果均为动物双歧杆菌

Responsible for constructional planning of clinical application research, and its implementation, along with the examination studies on intestinal tract micro ecology and hematological attributes of AIDS patients, which including the quantitative tests of Bifidobacterium, Lactobacillus, Bacteroides, Enterobacter, Escherichia coli and cl.perfringen in feces.

负责临床应用总体设计和临床应用研究，负责艾滋病人血液指标及肠道微生态学检测研究，其中包括：粪便中双歧杆菌、乳杆菌、拟杆菌、肠杆菌、肠球菌、产气荚膜梭菌数量的检测。

Before fed with antibiotics, the predominant intestinal aerobic and facultative anaerobic bacteria were Enterobacteriaceae, Flavobacterium, Acinetobacter and Vibrio.

投喂药饵前，鲤肠道好氧及兼性厌氧菌优势菌群为肠杆菌科、黄杆菌属、不动杆菌属和弧菌属；厌氧菌群中双歧杆菌属最多，乳杆菌属次之，拟杆菌属最少。

Meanwhile other lactic acid bacteria such as Lactobacillus plantarum, Lactobacillus bifermentum, Lactobacillus acetotolerans, Lactobacillus curvatus, Lactobacillus crispatus, Lactobacillus casei, Pediococcus pentosaceus, Pediococcus acidilactici, Leuconostoc were also identified.

同时也分离到植物乳杆菌、双发酵乳杆菌、耐酸乳杆菌、弯曲乳杆菌、卷曲乳杆菌、干酪乳杆菌、戊糖片球菌、乳酸片球菌、明串珠菌等多种乳酸细菌和德巴利氏酵母、球拟酵母等微生物。

According to the nutritional needs of B.bifidum,based culture and stimulated factors were optimized by quadratic design.The optimum based culture was as following: yeast extract was 0.9%,tryptone was 0.3%,soya peptone was 0.3%,glucose was 2.0%,bifidus was 0.8%,growth factor was 10%.

针对两歧双歧杆菌的营养需要，采用正交试验设计优化增殖培养基，结果表明，酵母膏是影响短双歧杆菌的显著因素，最佳的优化培养基配比是：酵母膏0.9%，胰蛋白胨0.3%，大豆蛋白胨0.3%，葡萄糖2.0%，双歧因子0.8%，生长因子10%。

These are foundation stones of biological classification of the genus Bifidobacteria. The designation of species and biotypes is on the basis of carbohydrate fermentation physiological characteristics and DNA-DNA homology studies.

在婴儿和成人的肠道、阴道和口腔以及许多动物的消化器官中都可检出双岐杆菌，它们可分为人类种群、非人类种群，并具有不同的生物型。1双岐杆菌的形态双岐杆菌是无运动性不产芽孢的杆菌，在不同条件下呈现不同的形态。

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢？

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我，一个心中布满虚荣的女孩子真实的想法。