双变性的

Two relevant sites of enzymatic digestion were added to the mTNF-α by PCR. The mTNF-α was linked to the 3'end of m/〓 in pGEX4T-1 vector. The prokaryotic expression vector pGEX4T-1m/〓-mTNF-α was constructed successfully. After induction and expression by IPTG, the expression of two kinds of fusion protein is 15% and 12% of total bacteria proteins respectively. The anti-HCC bifunctional antibodies m/〓-mTNF-α were identified by electrophoresis after the inclusion bodies were purified, denature, renature, re-purified, digested by thrombin and further purified.

采用PCR的方法在mTNF-α的两端加上所需要的酶切位点，将之连接在m／〓的3'端，构建原核表达载体pGEX4T-1 m／〓-mTNF-α，通过IPTG的诱导表达之后，两种融合蛋白的表达量分别占细菌总蛋白的15％、12％，表达产物经包涵体的纯化→变性→复性→纯化→凝血酶酶切→进一步纯化后，可以得到纯度为电泳纯的m／〓-mTNF-α抗肝癌双功能抗体。

Common transparent quartz tube SiO2 more than 99.9% purity, geometry, chemical stability, thermal stability, resistance to thermal denaturation and anti-crystallization properties of the fine, optical transmittance above 93%; from hydroxyl in hydroxyl tube below 10ppm, after vacuum from hydroxyl, hydroxy-up to 5ppm below apply to high-pressure mercury lamp, sodium lamp, metal halide lamp, such as low-hydroxy products; white pipe, used mainly for heating tube, heating tube, far infrared control the manufacture, milky quartz pearl quartz tube possession of new technology, the management side as if the surface of pearls, all aspects of performance than ordinary white tube better; filter tube to filter out ultraviolet UV band in the harmful wavelengths of 190-320nm ultraviolet light source applied to environmental protection manufacturing; no ozone UV filter tube and pipe the contrary, through the ultraviolet wavelength around 253.7nm, there is no ozone UV lamp main material, widely used in medical equipment, water treatment, food hygiene, such as sterilization; quartz casing diameter 8-120mm in between, has a single opening, double opening round, flat-top shape and widen the mouth of necking, flanging, sanding and other processing, high temperature resistance, corrosion resistance, good light transmission and moisture-proof, explosion-proof characteristics such as widely used in aquarium thermostat, disinfection equipment, heating pipes, water treatment, metal halide lamp, such as product manufacturing and environmental protection, health, chemical and other fields; quartz Clamshell specifications 8-180mm, the shape according to the customer requirements, the bubble shell can take capillary has to carry out the exhaust, high temperature, light transmission is good, mainly for JC, JD, H1-H7, the stage lights and high-pressure mercury lamp, sodium lamp, metal halide lamp, such as product manufacturing; quartz water jacket mainly used in printing and packaging processing industry ---- UV light-curing, UV light curing is a very reliable and can be used to replace hot air dryers and other drying technique of curing technology, which in the printing and packaging of the processing industry has been widely.

普通透明石英管SiO2纯度达99.9%以上，几何尺寸、化学稳定性、热稳定性、抗热变性和抗析晶性能优良，光学透过率达93%以上；脱羟管羟基在10ppm以下，经过真空脱羟，羟基可达5ppm以下，适用于高压汞灯、钠灯、金卤灯等低羟基产品；乳白管，主要用于发热管、加热管、远红外管的制造，珠光石英管乳白石英管之新工艺，管面好象珍珠的表面，各方面性能比普通乳白管更优；滤紫外线管能够滤除紫外光谱波段中对人体有害的190-320nm波长的紫外线，适用于环保光源的制造；无臭氧管与滤紫外线管相反，可透过253.7nm左右波长的紫外线，是无臭氧紫外线杀菌灯的主要材料，广泛用于医疗器械、水处理、食品卫生等杀菌；石英套管直径范围在8-120mm之间，有单开口、双开口、圆头、平头等外形和扩口、缩口、翻边、磨砂等加工，具有耐高温、耐腐蚀、透光性好及防潮、防爆等特点，广泛使用于水族恒温器、消毒设备、加热管、水处理、金卤灯等产品的制造和环保、卫生、化工等领域；石英泡壳规格Φ8-180mm，形状根据客户要求，泡壳上可以接有毛细管，可进行排气，耐高温，透光性好，主要用于JC、JD、H1-H7、舞台灯和高压汞灯、钠灯、金卤灯等产品的制造；石英水套主要用于印刷和包装加工工业---- UV光固化， UV光固化是一种十分可靠的、可取代热空气干燥机和其他干燥技术的固化技术，它在印刷和包装加工工业已经得到广泛应用。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！