双切的

The results indicated that DNase E belonging to endonuclease.Conclusion1. Established a preparation technics of rude extracts of Lumbricus Bimastus nucleases.

应用琼脂糖凝胶电泳对DNase E水解双链环状DNA后的产物进行鉴定，证明了DNase E属于核酸内切酶。

Methods Expression plasmid was constructed with DNA recombi nant technique.

方法采用DNA重组技术构建结核分支杆菌Ag85B基因的表达载体，双酶切和聚合酶链反应鉴定重组子，阳性重组子转化大肠杆菌，并诱导表达外源蛋白。

The mechanism of electromagnetic leakage is analyzed, and then the slotted shielded-twist-pair is regarded as a cascaded network.

分析了切缝屏蔽双绞线的电磁泄漏机制，并把其等效为级联网络。

There is four two-color automated printing machines, three slotting cutting paper machines, five disparting paper machines with thin slice, ten punchers in the cardboard department. It is one of the best scale cardboards and cartons producing factories in Jiangmen city.

纸箱车间现拥有全自动三色印刷机一台、双色印刷机四台，开槽切纸机三台，分纸机带薄刀片五台，打钉机十台；是目前江门地区最具规模的纸板、纸箱生产厂家之一。

Methods The total RNA was acquired from young rabbit particular cartilage, the BMP7 gene was inserted into pcDNA-3.1 to construct eukaryotic expression vector plasmid of pcDNA3.1-BMP7; the target gene-BMP7 was identified respectively by PCR analysis, restriction enounces analysis and nucleotide sequencing; the plasmid was transfected into adult rabbit knee articular chondrocytes, then the expression of BMP7 was detected by in situ hybridization, PCR and Western blotting.

从体外培养的幼兔膝关节软骨细胞中提取总RNA；按GenBank BMP7基因序列化学合成2条引物，采用RT-PCR方法得到BMP7基因；将BMP7基因片段插入到真核表达载体pcDNA 3.1中，构建pcDNA3.1-BMP7真核表达载体质粒；利用双酶切、PCR及核苷酸序列分析鉴定BMP7目的基因；将重组pcDNA3.1-BMP7真核表达载体质粒转染家兔关节软骨细胞，分别采用原位杂交、PCR、Western blotting法测定BMP-7表达。

AD models were made by transecting bilateral fimbria-fornix.

采用双侧切段穹隆海马伞的方法制备AD大鼠模型。

Objective To study the safety and efficacy of transurethral resection of bladder tumor under block anesthesia of bilateral obturator nerves.

文章摘要：目的探讨双侧闭孔神经阻滞在经尿道膀胱肿瘤电切术（transurethral resection of bladder tumor，TURBt）的安全性与有效性。

Connective reaction: 6.5μl PCR productions, pMD18-T vector 0.5μl, Ligation SolutionⅠ5μl, T4 DNA Ligase 0.5μl, 10×T4 DNA Ligase 1.5μl were connected overnight at 16℃. 5、transformation of vector: DH5α was prepared by CaCl and mixed with connecters, cultured on Amp+LB overnight at 37℃.

双酶切及PCR鉴定。1%琼脂糖凝胶电泳。7、序列测定：测序结果与Gene Bank中报道的序列同源性为99.1%，说明变链ldh基因及同源区基因已克隆成功；命名该重组质粒为pMD18-T-ldh。

Marcello Lippi has tipped Manchester United to do the double over Chelsea and land both the Premier League and Champions League.

马塞洛。里皮支持曼联击败切尔西拿到联赛和冠军杯的双冠王。

Objective To investigate the surgical results of anastomosis with double linear cutting staplersby comparing with DGIA with circular end-to-end staplers.

目的通过与管状吻合器肠吻合进行比较，分析双直线切缝器进行肠吻合的临床应用价值。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。