双切的

High-grade advanced machinery and equipment is assumed business hardware security, the people Langfang Founder Phototypesetting owned printing system, the two groups are the four American Oriental color back 48 reported high-speed offset rotary printing machine, two rotary Taipei production, the two Taiwan Strait Fort off four-color, two quarto four-color printing presses, a four-color printing octavo; Beiren producing two-color, single-, double-sided offset platforms five books, domestic laminating machine, cutting machine palpable , UV, indentation machine, as well as fine books, paperback double booking packages and other major equipment.

高档先进的机器设备是业务承担的硬件保障，廊坊人民印刷厂拥有北大方正照排系统、两组美国东方正四背四八色高速胶印轮转印报机、两台北人产轮转机、两台海德堡对开四色、两台四开四色印刷机、一台八开四色印刷机；北人产双色、单、双面平台书刊胶印机五台、国产复膜机、扪切机、UV、压痕机以及书刊精、平装双套装订机等主要机器设备。

Numerical results demonstrated that there are three kinds of the instability for the under-expanded supersonic jets according to their shock cell structure patterns:(1) the regular reflection shock cell with a single shear layer,(2) the Mach reflection shock cell with two shear layers and (3) the curved Mach stem with a relatively high expansion ratio.

计算结果表明欠膨胀超声速射流的失稳机制根据射流激波结构的特征可分为3种失稳模式：具有规则反射激波结构和单一剪切层特征的射流不稳定性；带有马赫反射激波结构和双剪切层特征的射流不稳定性；具有弯曲马赫杆和高度欠膨胀射流的不稳定性。

The N gene ORF was then subcloned into pET-30a vector and the recombinant plasmid was transformed into E.coli BL21 (DE3) and induced with IPTG. The protein expression was determined by SDS-PAGE. The expressed protein had a molecular weight of 54.4 Ku that existed as inclusion body. Thin-layer scanning showed that the expression product accounted for 30.5% of the total bacterial proteins. The recombinant protein possessed native biological activity and could react with anti-PEDV hyperimmune serum in Western blot.

以阳性质粒为模板，用分别含有BamH Ⅰ和Xho Ⅰ酶切位点的上、下游引物扩增得到ORF，其PCR产物经BamH Ⅰ和Xho Ⅰ双酶切后定向克隆到pET-30a载体，构建的重组质粒命名为pET-30a-PN；将pET-30a-PN转化到大肠杆菌BL21 (DF3)中，在IPTG诱导下进行表达；SDS-PAGE结果表明表达出与预期大小相符的约54.4 Ku的重组蛋白，重组蛋白以包涵体形式存在；薄层扫描结果表明表达产物占菌体总蛋白的30.5%；Western blot分析表明表达的重组蛋白能与抗PEDV高免血清反应，说明该重组蛋白具有免疫学活性。

Upper central incisors moved forward and downward together with maxillary, at the same time they upright in the sagittal plane and tip mesially. 3 The occlusion plane rotated clockwise for about 1.5°. 4 The width increasement effect of RME comes from approximately the equal amounts of skeletal and dental expansion. The mode of dental movement was a combination of body and tipping movements of posterior teeth.

结果：1后牙在宽度方向上为整体移动和倾斜移动相结合的移动方式，但第一磨牙和第一双尖牙稍有差别；牙齿和骨对宽度增加的贡献各占约1/2；2后牙在垂直方向有伸长的趋势；3第一磨牙和第一双尖牙均向前整体移动，轴倾度保持不变；4中切牙整体随上颌骨前部向前向下移动，其牙长轴发生直立，轴倾度增加；5上颌平面发生顺时针旋转。

Six hundred ova were transfered into twenty-nine pseudopregnant recipients. Fifty-five offspring were born from the ova mircroinjected with the Bcl-2 fragment expression cassette.Twelve of them were proved to be gene integration positive by PCR .Four founder mice,two male and two female ,were further confirmed to be transgenic by Southern hybridization.

用BglII+SalI+PvuI三酶切pWBS和用BglII+BamHI双酶切pWCS，分别回收并纯化3.0kb的基因片段WAP-Bcl-2-SV40polyA和3.5kb的基因片段WAP-c-myc-SV40poly，作为目的基因，通过受精卵显微注射技术，分别导入C57BL／6J雌鼠与DBA／2J雄鼠交配的F1代小鼠受精卵的雄原核中，各注射约1000枚，将存活的受精卵各600枚分别移植至29只假孕母鼠输卵管壶腹部内。

A novel double hairpin molecular beacon that has more stable structure than traditional molecular beacon has been designed. Its hairpin structure will not fully open when it hybridizes to cDNA but the hybrid can be cut by restriction endonucleases, which leads to destroy of its structure and restore the fluorescence.

本论文的主要内容包括以下三个方面：(1)基于双发夹分子信标分析核酸甲基化酶及药物筛选我们设计了新型分子信标-双发夹分子信标，该探针具有比传统分子信标更稳定的分子结构，与互补核酸链杂交后发夹结构不完全打开，但在酶切反应后其发夹结构被破坏，荧光又可恢复。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

This is impractical for engineering problem. It is necessary to break the shear layer in order to enchance mixing. It is very useful for combustor design with cavity or dual-cavity. In such combustor the fuel jet can break the

要使超声速混合增强，必须打破稳定的剪切层，燃烧室采用凹槽或双凹槽设计是一种行之有效的方法，它产生的燃料射流就能打破剪切层，从而使混合和燃烧效率进一步提高。

SLG series double screw extrusion machine adopts the most advanced technology of double screw expansion,to make the material reach the states of being expanded,cured and texturized through high temperature and high pressure and high shearing.

SLG系列双螺杆膨化机采用国际先进的双螺杆膨化技术将物料经过高温、高压、高剪切，使物料膨化以达到膨化、熟化或组织化的状态。

The results of transient transfection showed that the recombinant plasmid could be lowly expressed in the murine macrophage cell line RAW264.7 and murine melanoma cell line B16, both of which can endogenously express Tim-3, but not in the African green monkey kidney cell line COS-7 in which no endogenous Tim-3 could be detected.

结果 pGL3-Tim-3P2经PCR、双酶切及DNA测序分析鉴定正确无误；pGL3-Tim-3P2转染RAW264.7和B16细胞（两种细胞均可表达内源性Tim-3）24 h和48h，双荧光素酶活性检测显示其启动子相对活性约为pGL3-Basic空载体的2倍；而pGL3-Tim-3P2转染不表达内源性Tim-3的COS-7细胞后检测不到荧光素酶活性。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。