双切的

Analyzing T-DNA flanking sequences isolated by TAIL-PCR revealed that the T-DNA nick position in the tagged rice plants are more complex than in tansgenic dicot species as tobacco and Arabidopsis.

分析TAIL-PCR分离的T-DNA侧翼序列，发现水稻转化体中T-DNA右边界的剪切位点比转基因拟南芥和烟草等双子叶植物中T-DNA右边界的剪切位点要复杂得多。

In this paper,die design for cut tongue bending part with double inclined plane and knife mouth are introduced,for cut tongue bending part with single plane.

通过理论分析，介绍了双斜面刃口切舌弯曲模的设计方法及单面切舌零件的卸料及防止垂直切口撕裂的方

Minter's paintings were included in the 2006 Whitney Biennial along with billboards in Chelsea that coincided with the Biennial.

铭泰的画被列入2006年惠特尼双年展伴随着广告牌，与切尔西的双年展不谋而合。

Then pMD-P80 is digested by the enzymes Xho I and Apa I, separated and linked to the linear plasmid pEGPF-C1 after digested by Xho I and Apa I. So the recombined expression plasmid pGFP-P80 is achieved. Then it is sequenced, PCR and digested by the restriction enzymes Xho I and Apa I. The results give that pGFP-P80 is achieved successfully and the insert sites, direction and reading frame are all right. It builds the basis for expressing, purifying, gaining p80 protein from mammiferous cells.

将pMD-P80 分别经Xho I 和Apa I 双酶切和回收，然后与经过Xho I 和Apa I 酶解的真核表达载体pEGPF-C1 连接、转化，获得重组质粒，经PCR，Xho I 和Apa I 限制性酶切和序列测定，鉴定为真核表达质粒pEGFP-P80，并且目的基因的插入位置、方向和读码框完全正确，为在哺乳动物细胞中表达并分离纯化p80 蛋白奠定了基础。

And then, the pBI101.2 wirelike vector and the promoter fragment of 886bp were acquired. Becase the promoter was modified by the HindⅢand BamH I, the acquired target genes fragment, digested by the restriction endonuclease, was matched to the wirelike vector.

由于在启动子两端有人工设计合成的HindⅢ和BamHⅠ酶切位点，经上述双酶切消化后，回收目的基因片段与线状载体的粘性接头恰好匹配。

Product main business: 1, 1400 ~ 2500mm, single, three, five, seven corrugated cardboard production line 2, 2200 ~ 3500mm, double, three, four, five-color printing slotting die-cutting machine three, 2400 1200mm automatic visco-box machine , semi-automatic machine stick me four, all kinds of specifications±sub-machine semi-automatic paper isobar nail box machine, paper balers, etc. Friday, 800 ~ 1620 mm automatic platen die-cutting the six-ping, 1100 ~ 1450mm automatic paper mounting machines, seven semi-automatic paper mounting machine, a variety of bundlers, eight winding machine, all kinds of specifications, types of testing equipment carton¨Buck Shanghai Machinery Co., Ltd. of China's packaging industry and the packaging machinery market very well.

主要经营产品：一、1400~2500mm ，单、三、五、七层瓦楞纸板生产线二、2200~3500mm，双、三、四、五色印刷开槽模切机三、2400×1200mm 全自动粘箱机、半自动粘箱机四、各种规格的薄刀分纸压线机半自动钉箱机、废纸打包机等五、800~1620 mm 全自动平压平模切六、1100~1450mm全自动裱纸机、半自动裱纸机七、各种规格的捆扎机、缠绕机八、各种规格、类型纸箱检测设备上海川斯霸克机械有限公司对中国包装行业及包装机械市场非常了解。

We have advanced machinery such as ink printing machine, two-color and three-color presses, four-color press grooving machine, Rotary press breaking angles cutting machine, paper sheet cutters, packaging machines, paper press machines, die cutter and battery plastic equipment.

本厂拥有先进的水墨、双色、三色印刷机、四色印刷开槽机、轮滚切角机、分纸机、装钉机、裱纸机、压纸机、模切机、电池塑料底圆设备等一系列完善的纸箱、纸品制造机械。

Has advanced production equipment and testing equipment, strong technical force, the main products are various sizes of dry cars, trucks kiln and drying cars, vacuum Yingsuzhuanji, efficient biaxial blenders, roll-on, cut blanks, cutting And other equipment, products widely used in mining, building materials, metallurgy, metal and other industries.

拥有先进的生产设备及检测设备，技术力量雄厚，主导产品有各种尺寸的干燥车、窑车、烘干车、真空硬塑砖机、高效双轴搅拌机、对辊机、切坯、切条机等设备，产品广泛应用于矿山、建材、冶金、五金等行业。

This Belt is used to transport the slugs to the belt of Piece-Cutting Machine with Chamfering Device.

主要作用是将双泥条垂直切条机切割后的泥条，输送到带倒角切坯机的输送皮带上。

Methods(1) Construction of plant expression vector containing RS geneThe cloning vector pT-RS was constructed containing RS gene extracted from the leaf tip of Vitis vinifera by PCR amplification and RS sequence was identified by PCR amplification,enzymes digestion and sequence analysis.The plant expression vector containing RS gene was obtained and RS sequence was identified by PCR amplification and double enzymes digestion too.

研究方法(1)含白藜芦醇合酶基因的植物表达载体的构建提取葡萄基因组总DNA，通过PCR扩增得到RS基因，将此基因连接到克隆载体pGEM-T Vector，得到重组载体pT-RS；经PCR、酶切及序列分析鉴定后，将RS基因克隆到植物表达载体pBI121，得到重组载体pBI-RS，用PCR及双酶切方法进行鉴定。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。