双切的

This paper also describes the conditions for ensuring two engagement lines with double concave-convex circular arc profile.

讨论了中心距偏差和切深偏差相互补偿问题。文中还讨论了双圆弧齿形保持双啮合线的条件。

Falciparum isolates prepared directly from 5 cases of cerebral malaria patients'blood in Mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding the regions 12—17 in MAD20 merozoite surface protein 1(MSP1) of Papua New Guinea strain of P . falciparum were used as primers. The PCR products were digested with EcoRI and KpnI, respectively, and the generated fragment regions 16—17 were cloned into M13mp18 and M13mp19 vectors and their DNA were analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应技术对中国5 例脑型疟患者恶性疟原虫云南省勐腊县勐罕CMH/YN 分离株和云南省盈江县农场CYJ/YN 分离株基因组DNA 裂殖子表面蛋白1 (MSP1)第13—17 区基因进行扩增，将扩增产物分别经EcoRI 和KpnI 双酶切后，回收的MSP1 第16—17 区基因分子定向克隆M13mp18 和M13mp19 载体，按Sanger 双脱氧链终止法进行DNA 序列测定，并与MAD20、K1 和Wellcome 株原型基因进行同源性分析比较。

Methods Genomic DNA samples of two isolated Plasmodium falciparum isolate strains prepared directly from 5 cases of cerebral malaria patients′ blood in mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding FC27 merozoite surface protein 2 (MSP2) of Papua New Guinea strain of Plasmodium falciparum were used as primers. The PCR products were digested with BamH1 and Hind Ⅲ respectively, and the generated fragment MSP2 were cloned into M13mp18 and M13mp19 vectors and their DNA was analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应对5例中国脑型疟患者恶性疟原虫云南省勐腊县勐罕分离株和云南省盈江县农场分离株基因组裂殖子表面蛋白2(MSP2)基因进行扩增，将扩增产物分别经BamHI和Hind Ⅲ双酶切后，回收的MSP2基因分子定向克隆M13mp18和M13mp19载体，按Sanger双脱氧链终止法进行DNA序列测定，并与恶性疟原虫株FC27、K1、IC1和CAMP株进行同源性分析比较。

PCR-RFLP in FSHR gene 5'–flanking region with exon 1 digested by restriction endonuclease TaqⅠdemonstrated that the frequencies of allele A (0.5500) and genotype AA (0.5000) in twinning cows were higher than that of allele A (0.3500) and genotype AA (0.3000) in monovular cows for Qinchuan cattle, while The frequencies of allele B (0.5937) and genotype BB(0.6875) in twinning cows were higher than that of allele B (0.5416) and genotype BB (0.4167) in monovular cows for Holstein cattle. Key words: Bovine; Twinning; FSHR gene; PCR-RFLP

FSHR基因5'端（包括侧翼序列和第一外显子）的限制性内切酶TaqⅠ的PCR-RFLP标记研究发现，在秦川牛中双胎母牛的A基因频率（0.5500）和AA基因型频率（0.5000）分别高于单胎母牛的A基因频率（0.3500）和AA基因型频率（0.3000）；在黑白花奶牛中双胎母牛的B基因频率（0.5937）和BB基因型频率（0.6875）均高于单胎母牛的B基因频率（0.5416）和BB基因型频率（0.4167），也就是FSHR基因的5'端的PCR-RFLP标记在一个品种内单胎母牛和双胎母牛之间有一定趋势，但两个品种的趋势不同。

Zhejiang Xinda Printing Co., Ltd After ten years of development, has become the scale, the grade of the packaging and decoration printing enterprises, the past two years several technological transformation, from Germany to introduce CP2000 Heidelberg Speedmaster-type CD102 1 Taiwan, CD102 (off four-color), one Taiwan, SM74 (quarto four-color), one Taiwan, SM52, one Taiwan, SM52 (octavo four-color), one Taiwan, and Taiwan Bi Sheng automatic die-cutting machine , tipping machine, folder gluer, BAG paste machine, paste-mounted machine; UV polishing machine, pressure-ray machine, dual-use laminating machine, Corrugating Machine, hydraulic cutter program, books and binding machines, Germany ......

浙江新大印刷有限公司历经十多年的发展，已成为上规模、上档次的包装装潢印刷企业，近两年来几次技改，从德国引进海德堡速霸CP2000型CD102一台、CD102一台、SM74一台、SM52一台、SM52一台，以及台湾铋晟全自动模切机、烫金机、糊盒机、贴袋机、贴裱机；UV上光机、压光机、双用复膜机、瓦楞机、液压程控切纸机、书刊装钉机、德国

Thereafter,DNA fragment of Hyl-1-Amp-Hyl-1 was subcloned into pBR322 vector,the resulting construct was then checked by PCR and restriction analysis for the proper configuration of the knockout vector pBR322-SA.

该载体与质粒pBR322分别用EcoRⅠ、PstⅠ双酶切后进行连接，得到基因敲除的重组载体pBR322SA，PCR 及限制性酶切分析，构建的敲除载体与设计结果相符。

The main jobs of this paper are as followed: 1 The reuse of the frame design technology for aircraft assembly fixtures is researched. a frame line part library is built with the templet driven technology, the reuse of the frame line part come true by the tools for instantiation and instance modification. The design resources are effectively used, some repeating work is cut. 2 Two methods to obtain the assembly feature are proposed: the straight way by the Enumeration method and the indirect way by project features to the assembly plane. All of the assembly features of the frame and the adjustable bearings are obtained by these two methods which makes the frame automatic assembly technology for aircraft assembly fixtures come true. 3 The frame automatic assembly technology aircraft assembly fixtures based on assembly features is researched. A binary tree assembly model is used to express the assembly information of the frame for aircraft assembly fixtures, the automatic assembly between the frame, the aircraft and the adjustable bearing is completed within the three steps: distribute, obtain and evaluate assembly feature. Finally, the efficiency of the frame assembly design is proved. 4The frame interpart modeling technology for aircraft assembly fixtures is researched, the intersection methods are concluded and the arithmetic of the single-cut, double-cut and inter-cut intersection is proposed, the interpart fast modeling of the frame design for aircraft assembly fixture come true. 5Based on the researches mentioned before, a sub-system of frame fast design for aircraft assembly fixture is developed on CATIA. The sub-system is used to design the aircraft assembly fixtures in a large aeronautics corporation and make a good- effect.

论文的主要工作如下： 1研究了飞机装配型架骨架设计重用技术，在总结归纳骨架线框结构类型的基础上，应用模板驱动的方法建立了骨架线框库，通过骨架线框零件的实例化和离线编辑实现骨架线框的重用，有效利用设计资源，减少重复劳动； 2提出穷举几何对象的装配特征直接获取方法，遍历零件模型的几何对象，从中捕捉所需装配特征，该方法简捷、高效；提出向装配面投影的装配特征间接获取方法，该方法在现有特征基础上生成装配特征，解决了部分装配特征难以直接获取的问题； 3研究了基于装配特征的飞机装配型架骨架自动装配技术，采用一种二叉树装配模型表达骨架的装配关系，并通过装配特征的分配、获取和赋值三个步骤，实现了骨架分别与飞机产品和可调支承的自动装配，避免了繁琐的人工交互操作，提高了骨架设计的效率和规范化程度； 4研究了飞机装配型架骨架零件间建模技术，在总结归纳骨架元件相贯处理方式的基础上，提出了骨架元件单剪切相贯、双剪切相贯和互剪切相贯的实现算法，简化了骨架相贯处理的过程； 5基于以上研究，在CATIA平台上开发了飞机装配型架骨架快速设计子系统，并应用于某大型航空企业的装配型架设计，提高了设计效率和设计质量。

Main business: CNC engraving machine Series: Applications: stainless steel, copper engraving Case insets processing; stainless steel, copper Case Shouban; spherical, Globoidal processing, text, sculpture ★ Case 5-axis double-headed insets engraving machine ★ Case 5-axis three insets engraving machine ★ Case 4-axis linkage double-headed insets engraving machine ★ Case 4-axis linkage three insets engraving machine ★ high triaxial strong double-headed die machine ★ high strength triaxial 3 Die machine ★ Case Drilling Machine, surface drilling machine ★ Case-meter pit, watches and clocks, glass-processing machine production testing of aircraft series:● test the waters Series: 10 automatic machine manually test the waters, six automatic Manual examination of water, really test the water leak-loaded needle ● Series: Semi-automatic assembly line of watches and clocks, watches and clocks, with three-pin CNC machines, semi-automatic machine ● single needle assembly Series: Semi-automatic cutting the core of machines, Case glass adhesive glue Machines, pneumatic pressure Digai ● strap-test: strap Rally torque testing machine, clasp switch testing machine, strap swing testing machine ● first test table: Table heading to the functional testing of aircraft, form the first vibration test machines, Table first parabolic dynamic testing machine has been with the German company Rexroth, Japan Yasukawa, Panasonic, Mitsubishi, and other well-known international companies have a long-term stability of technical cooperation, continue to study and absorb the advanced technology at home and abroad.

主营业务： CNC雕刻加工机系列：应用：不锈钢、铜表壳镶石雕刻加工；不锈钢、铜表壳手板；球面、弧面加工、文字雕刻★表壳五轴联动双头镶石雕刻机★表壳五轴联动三头镶石雕刻机★表壳四轴联动双头镶石雕刻机★表壳四轴联动三头镶石雕刻机★高强力三轴双头模具加工机★高强力三轴三头模具加工机★表壳钻孔机、表面钻孔机★表壳米坑位、玻璃位加工机钟表生产测试机系列：●试水系列：十头自动手动试水机、六头自动手动试水机、真水试漏机●装针系列：半自动钟表装配生产线、数控钟表装三针机、半自动单针机●装配系列：半自动切把芯上的机、表壳玻璃胶水粘接机、气动压底盖机●表带测试：表带拉力扭力测试机、表扣开关测试机、表带摇摆测试机●表头测试：表头按的功能测试机、表头震动测试机、表头抛动测试机一直以来，公司与德国Rexroth、日本安川、松下、三菱等国际知名企业有着长期稳定的技术合作，不断地学习和吸收国内外的先进技术。

The company has about 700 employees, the factory covers an area of about 15,000 square meters, plant area of 20,000 square meters to over the past few years development, already have a certain scale, the printing workshop of existing four-color Heidelberg presses, Mitsubishi four-color, five-color presses, Akiyama four-color printing presses, two-color Heidelberg presses, single-color machine, the entire computer paper cutter, plate-making equipment group; printed paper mounting after the workshop has a fully automatic machines, semi-automatic on the mounted machine , automatic water-based UV machine, fully automatic UV machine, polishing machine, pressure-ray machine, laminating machine, automatic rolling box machine, semi-automatic folder gluer and other equipment: fine line between many existing automatically be on the white plastic plastic machine, all-purpose adhesive on the glue machine, beer machine, cutter, sawing machine, grinding machine, gong machine, towel angle machine, tipping machine, sewing machine, angle cutter, portable drilling machine, wait until the equipment corns machine.

公司现有职工约700人，厂区占地面积约15000平方米，厂房使用面积20000平方米，以过几年来的发展，已拥有一定的规模，印刷车间现有海德堡四色印刷机，三菱四色、五色印刷机，秋山四色印刷机、海德堡双色印刷机、单色机、全电脑裁纸机、制版设备一批；印后车间拥有全自动裱纸机、半自动对裱机、全自动水性UV机、全自动UV机、上光机、压光机、覆膜机、全自动轧盒机、半自动糊盒机等设备：精品国间现有自动流水线多条，白胶上胶机、万能胶上胶机、啤机、切纸机、锯床、打磨机、锣机、巾角机、烫金机、针车、切角机、笔记本打孔机、鸡眼机等到设备。

The main products: printed, PC Refeng eagerly machines, closed-end, the closure of the bilateral closure bag machine, dry-type solutions, multi-purpose PE, PO, PP blown film, stretch film machine, computer - Pockets, vest-pocket cut bags, even vol-off point, R-series; Zhenzhu Mian foam machines whole plant equipment including foam extruder, Fumo machines, complex machines, straight cutting machines, and other family Recycling .

主要产品：凹版印刷机、电脑热封热切机、底封机、中封机、双边封制袋机、干式复合机、多用途的PE、PO、PP吹膜、拉伸膜机、电脑平口袋、背心袋切袋机、点断连卷机、R机等系列；珍珠棉发泡机整厂设备包括发泡挤出机、复膜机、复合机、直切机、回收再生机等系列。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。