双倍

In the present study, the autotetraploid rice IR36-4X was treated with ion implantation technique by nitrogen ion beams and a polyembryonic seedling mutant (named as the IR36-Shuang) was identified in M2 generation. The mutant line was systematically investigated about the location of seedling in polyembryonic seedling, and the cytological initiation of the additional embryo in the 1R36-Shuang was observed and determined.

以低能氮离子束为诱变源，通过对同源四倍体水稻品系&IR36-4X&进行离子注入后在其第2代群体内筛选得到了1株具有多胚苗性状特征的突变株（IR36-双），对该突变株后代的多胚苗形态特征及其多胚来源进行了研究。

The pured mammary epithelial cell line could be obtained by 2~3 passages. 2.Using RPMI1640 containing 10黃, 100IU/ml Penicillin and 100IU/ml Streptomycin as basic media, several different growth factors such as insulin, hydrocortisone, insulin-like growth factor, EGF and so on, were added and selected to culture goat mammary epithelial cells.

以RPMI1640 为基础培养液，山羊乳腺上皮细胞在含血清、双抗的同时，添加氢化考的松、Insulin、IGF-1、E2、EGF 及ITS 的不同组合，以细胞生长群体倍增值为参考标准，评估不同组合的生物活性因子对山羊乳腺上皮细胞生长的影响。

The pured mammary epithelial cell line could be obtained by 2~3 passages. 2.Using RPMI1640 containing 10%FCS, 100IU/ml Penicillin and 100IU/ml Streptomycin as basic media, several different growth factors such as insulin, hydrocortisone, insulin-like growth factor, EGF and so on, were added and selected to culture goat mammary epithelial cells.

以RPMI1640 为基础培养液，山羊乳腺上皮细胞在含血清、双抗的同时，添加氢化考的松、Insulin、IGF-1、E2、EGF 及ITS 的不同组合，以细胞生长群体倍增值为参考标准，评估不同组合的生物活性因子对山羊乳腺上皮细胞生长的影响。

Transcriptional activity of ACL recombinants normalized by Renilla was significant difference with pGL3-Basic expect for construct -27 and -15 (P＜0.01). Construct -919 contains highest activity. 3 times reduction of transcriptional activity from-919 to -679bp indicated that negative regulation factors located probably in this region. The activity started on construct -73 suggested the basal promoter activity was located within the -73 to +77bp region; Transcriptional activity of IDHβrecombinants was not significantly different between the recombinant -58 and pGL3-Basic. The activity started on construct -82, decreasing with the length of the fragment up to -164 in despite of a bit of fluctuation, and kept increasing from construct -164 up to -279. Thus, the basal promoter activity was located within the -82 to +16bp region, whereas the upstream 197bp conferred maximal transcriptional activity.

采用5'端缺失策略，结合启动子预测结果，分别构建了8个ACL基因和19个IDH3β基因启动子区重组子，将其转染猪PK15细胞系，并利用荧光素酶双报告基因系统检测了它们的活性，结果表明：在所构建的猪ACL基因5侧翼序列的8个重组子中，除pGL-ACL27和pGL-ACL15外，其它重组子的荧光素酶活性与阴性对照均达到极显著水平(P＜0.01)，表明它们均具有启动子活性，pGL-ACL919活性最强，到pGL-ACL679活性下降了将近3倍，说明从-919bp到-679bp区域可能存在负的调控位点，从pGL-ACL621到pGL-ACL73活性有小幅度下降，到pGL-ACL27活性降到与阴性对照水平无显著差异，表明维持该启动子的基本活性区域位于-73bp到+77bp之间；IDH3β基因启动子活性开始于pGL-IB82，然后虽然有些波动，但是活性一直下降直到-164bp处，之后活性又开始升高，直到-279bp处活性达到最高，这些表明维持该启动子的基本活性区域位于-82bp到+16bp之间，从-82到-279之间的179bp区域具有最高的启动子活性。

To determine the free drug,three methods were compared on two-colorimetric UV spectrophotography,Bradford and two...

在吸收度和线性均良好的情况下，双波长考马斯亮蓝G-250染料法的检测范围为0.25~32μg.mL-1，与Bradford法的检测范围(5~80μg.mL-1)相比，检测灵敏度提高了20倍，检测范围更宽。

For example,α2-macroglobulin appears to have an alternative structure that consists of a tetrameric subunit linked via disulfide bridges.

例如，第二号甲型巨型球蛋白似乎拥有一个由双硫键所连结的四倍体次单元所组成的结构。

Regulating the control group used traditional western medicine treatment: The application of the latest "chronic congestive heart failure treatment recommendations"China cardiovascular disease magazine, 2002; 30 (1 :7-21 Recommend the most appropriate method of treatment, According to patients after hospital-based treatment of illness:① general routine treatment: rest, salt restriction, oxygen, etc.;② to the diuretic (urine dihydrochloride grams thiadiazole 25 ~ 50 mg or 20 mg furosemide, antisterone 20 mg, 1 to 3 times daily oral);③ ACEI:(captopril 625 mg, twice daily beginning);④β-receptor blocker (metoprolol from 6.25 mg daily oral dose of 1 start);⑤ cardio (digoxin 0.25 mg daily oral administration, a week after the condition of patients based on the alignment of their drug dosage or suspension).

对照组采用西医规范治疗：采用最新的《慢性充血性心力衰竭治疗建议》（中华心血管病杂志，2002；30（1）：7—21）推荐的最适合的治疗方法，患者入院后即根据病情进行基础治疗：①一般的常规治疗：休息、限盐、吸氧等；②予利尿（双氢克尿噻25~50mg或速尿20mg、安体舒通20mg，每日1~3次口服）；③ACEI：（开搏通6.25mg，每日2次开始）；④β一受体阻滞剂（倍他乐克从6.25mg，每日1次口服开始）；⑤强心（地高辛0.25mg，每日一次口服，一周后依据患者的病情，调整其用量或暂停药）。

In order to analyze the conservation of maize centromeric satellite DNA and centromeric retrotransposon in the subspecies and relatives of Zea mays, dual fluorescence in situ hybridization was used to detect the existence and distribution of the above two repetitive sequences in Zea mays ssp. mexicana, Z. diploperennis, Z. perennis, Tripsacum dactyloides, Coix lacryma-jobi, and Sorghum bicolor.

为分析玉米着丝粒卫星DNA和着丝粒反转录转座子在玉米种的亚种及近缘种中的保守性，采用双色荧光原位杂交检测了这两种重复序列在墨西哥玉米、二倍体多年生类玉米、多年生类玉米、摩擦禾、薏苡、高粱中的存在和分布。

The latter has only one PL band peaking at about 1.48eV,while the former is a double-band PL spectrum with peaks at both 1.48eV and 1.97eV.The electroluminescenceform the Au／scored Si-rich SiO2 film／p-Si sample is about 6 times in intensity of that of the Au／unscored one.

未刻划样品的PL谱只有一个峰，位于840nm(1.48eV)，而刻划样品的PL谱是双峰结构，峰位分别位于630nm(1.97eV)和840nm.800℃退火的刻划富硅二氧化硅/p-Si样品在背面蒸铝制成欧姆接触和正面蒸上半透明金电极后在正向偏压10V下的电致发光强度约为同样制备的未经刻划样品在同样测试条件下的EL强度的6倍。

What Heppler didn't know is that out in thefree world,there is a larger urban global culturethat regards sneakers with the same highesteem—a streetwear-savvy population thatwill pay hundreds for the right shoe.Peoplewho post digital pics of their daily footwearchoices on message boards.Adult coupleswho save their best "deadstock" for their wedding days.Arty-sporty girlswho paint Nike swooshes on their bedroomwalls.Kids who camp outside urban boutiquesthe nights before limited-edition releases drop,so they can slap down $100-plus for a raritythey'll probably either put in storage or resell forthree times more on eBay.

不过那时候的海普勒并不知道，在外面自由的世界里正流行着一种范围更大的全球城市文化，对于球鞋，人们同样怀着崇高的敬意——他们对于街头服饰极其敏感，会为买到一双心仪的球鞋花上数百美元；他们会把自己每天所选择的各款球鞋拍成数码照片，贴到网站的留言板上；成年夫妻甚至会将他们最好的&多年珍藏&在结婚纪念日拿出来穿；拥有艺术细胞和运动细胞的女孩们会把耐克的&勾&形logo用油漆刷到卧室的墙上：在一些限量版球鞋发布的前一天晚上，孩子们为了排队，会在城里的专卖店门口宿营彻夜等候，直到啪地—声扔下100多美元拿走&稀世珍品&，然后要么把他们束之高阁，要么在eBay上以原价三倍再度转手。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。