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In this study, we found interesting two-cell stage embryos, which had a monopolar spindle in one blastomere and a bipolar spindle in the other during the second mitosis in a batch subjected to tetraploidization treatment.

在诱导四倍体过程中,我们发现了有趣的二细胞期胚胎现象,在第二次有丝分期间,它的一个分裂球中有一个单极纺锤体,二另一个有一个双极纺锤体。

If 〓mRNA positive expression, its DNA heteroploid type display as triploid or hypodiploidy, if 〓mRNA negative expression, its DNA heteroploid type display as hyperdiploidy、tetraploid or Bistem line. These from one flank demonstrate the role of 〓 gene suppress the proliferation of malignant tumor

表达阳性者,其DNA异倍体主要表现为三倍体或低二倍体,〓表达阴性者,其DNA异倍体主要表现为高倍体、四倍体和双干系,从一个侧面显示出〓基因的抑制恶性肿瘤细胞增殖的作用。

There was big difference among the 22 different materials in the disease resistance. Translocation lines Pm97033 showed the highest resistance in reaction than others. The percentage of diseased root and severed level were 11.3% and 5.4%; substitution lines Wan7107 showed medium resistance to the fungus, the percentage of diseased root and severity level were 21.4% and 10.6%; F5 generation of common wheat with Till showed higher resistance to take-all fungus than the PS progenies of common wheat with Aegilops tauschii or the other common wheat varieties.

菌饼+菌粒法对宁春4号的致病力明显高于菌饼法和菌粒法,其病根率、严重度和病茎率分别达到100.0%,57.3%和28.6%。22个遗传背景不同的小麦材料间抗病性差异较大,其中小麦-簇毛麦易位系Pm97033的抗病性最强,病根率和严重度分别为11.3%和5.4%;代换系Wan7107次之,病根率和严重度分别为21.4%和10.6%;硬粒小麦-簇毛麦双二倍体(TH1)与普通小麦品种杂交后代的抗病性强于粗山羊草与普通小麦品种杂交后代。

DNA C-values might be one of important indices in the evaluation of angiospermous invasiveness. To test this viewpoint, we compared DNA 1C-values and basic genome sizes in 3,676 angiosperms. The results suggest that:(1) the two nuclear values vary greatly among different groups, e.g., significantly higher in herbs than in trees, in monocots than in dicots, in perennials than in non-perennials;(2) the two nuclear values both have significant effects on plant invasiveness, especially in herbs, dicots, monocots, perennials, non-perennials, diploids, polyploids, Compositae, and Poaceae, while not significant in trees and Fabaceae.

DNA C值与被子植物入侵性密切相关,为考察应用DNA C值评估被子植物入侵性的可靠性和局限性,我们统计分析了全世界范围内3676种被子植物的DNA 1C值(配子未发生DNA复制时的染色体DNA含量)及基因组大小(单个染色体组的DNA 含量),结果表明:1)DNA 1C值和基因组大小在被子植物不同类群中差异极大,例如在草本植物中显著高于木本植物,单子叶植物显著高于双子叶植物,多年生植物显著高于非多年生植物;2)两项指标都与被子植物入侵性显著负相关,尤其是在草本植物、双子叶植物、单子叶植物、多年生植物、非多年生植物、二倍体、多倍体、菊科和禾本科中,然而在木本植物、豆科植物中其与植物入侵性无显著关系。

In the studies on transferring and identifying alien chro-mosomes into wheat,the combined use of mitotic and meioticchromosome N-banding is also successfully practiced in deter-mination of T.durum-H.villosa amphipoid,five wheatalien disomic additions with H.villosa chromosomes,〓〓,alien sulstitution line with chromosome 〓 andsome translocation lines.

在外源染色体导入、鉴定研究中,利用根尖有丝分裂中期染色体和花粉母细胞减数分裂中期Ⅰ染色体的N-分带,快速、准确地鉴定了硬粒小麦一簇毛麦双二倍体、普通小麦一簇毛麦异耐加系V〓、V〓、V〓、V〓和V〓,普通小麦一簇毛麦异代换系V〓以及一些具有易位的杂种后代。

By phenotype observation, combined with root tipchromosome counting, PMC MI pairing analysis and chromosome C-banding, a ditelosomic addition line 94K227 and a disomic addition line 94K280 was identified among BC2F2 and BC2F3 population respectively.C-banding results showed that 94K227 contains 1 pair of long arms in chromosome B, and 94K280 contains 1 pair of chromosome D from R.

用普通小麦-纤毛鹅观草双倍体与普通小麦中国春连续回交两次,然后连续自交,通过形态观察、根尖细胞染色体计数、花粉母细胞减数分裂中期Ⅰ染色体配对分析及染色体C-分带,在BC2F3和BC2F3群体中,分别筛选到一个端二体异附加系94K227和一个二体异附加系94K280,其中C-分带显示94K227添加的是纤毛鹅观草染色体B的一对长臂, 94K280添加的是纤毛鹅观草的一对染色体D。

In this study,Amplified fragment length polymorphism analysis was carried out by using 23 pairs of EcoRI-NN/MseI-NNN selective primers in amplification of the DNAs from reciprocal F_1 hybrids of Cucumis hystrix Chakr.and C.

为了研究细胞质背景对基因组序列丢失的影响,利用23对Eco RI-NN/Mse I-NNN选择性引物对Cucumishystrix与栽培黄瓜C.sativus的正反交F_1、异源双二倍体及亲本DNA进行AFLP分析。

In order to analyze the conservation of maize centromeric satellite DNA and centromeric retrotransposon in the subspecies and relatives of Zea mays, dual fluorescence in situ hybridization was used to detect the existence and distribution of the above two repetitive sequences in Zea mays ssp. mexicana, Z. diploperennis, Z. perennis, Tripsacum dactyloides, Coix lacryma-jobi, and Sorghum bicolor.

为分析玉米着丝粒卫星DNA和着丝粒反转录转座子在玉米种的亚种及近缘种中的保守性,采用双色荧光原位杂交检测了这两种重复序列在墨西哥玉米、二倍体多年生类玉米、多年生类玉米、摩擦禾、薏苡、高粱中的存在和分布。

The behavior of chromosomes at meiosis of PMC of 9 combination and selfed seed set of 16 combination of Triticum-Aegilopsamphidiploids were studied.

对小麦-山羊双二倍体9个组合的细胞遗传学和16个组合的自交结实率进行了观察。

Employing high-dilute method and 5-fold excess amine prepared two new "two-armed" acyclic diamine intermediates I_a and I_b, which are also intermediates of synthesizing microcyclic compounds, and eight aromatic Schiff bases, II_, were synthesized from reaction of I_a or I_b condensing with different aromatic aldehydes. Their composition and structure were characterized by Elemental analysis, IR, UV-Vis, LIF,~1HNMR and MS. The hydrogen bond interactions and MS fragmental patterns were also discussed.3. Several novel transition metal complexes using II_a as ligand were synthesized and identified by Elemental analysis, IR, UV-Vis,~1HNMR and molar conductivity.

1,采用高度稀释及胺过量五倍的方法合成了两个具有吡啶双酰胺臂的非环二胺Ⅰ_a和Ⅰ_b,并以它们为中间体进一步合成了八个芳香席夫碱Ⅱ_a-Ⅱ_h,通过元素分析及各种波谱测试鉴定了它们的组成与结构,对化合物的UV-Vis、LIF、IR、~1HNMR及MS进行了较为详细的讨论,还探讨了席夫碱的氢键相互作用及质谱裂解方式; 2,以席夫碱Ⅱ_a为配体合成了几个新型的过渡金属配合物,并通过元素分析、UV-Vis、IR、~1HNMR、摩尔电导及DTA—TG对其进行了表征,讨论了化合物的配位情况及配体在配位中的脱质子行为。

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Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时,他斥责什叶派民兵领导人对中央集权的挑衅行为。

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