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双二倍体

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In this study, we found interesting two-cell stage embryos, which had a monopolar spindle in one blastomere and a bipolar spindle in the other during the second mitosis in a batch subjected to tetraploidization treatment.

在诱导四倍体过程中,我们发现了有趣的二细胞期胚胎现象,在第二次有丝分期间,它的一个分裂球中有一个单极纺锤体,二另一个有一个双极纺锤体。

By phenotype observation, combined with root tipchromosome counting, PMC MI pairing analysis and chromosome C-banding, a ditelosomic addition line 94K227 and a disomic addition line 94K280 was identified among BC2F2 and BC2F3 population respectively.C-banding results showed that 94K227 contains 1 pair of long arms in chromosome B, and 94K280 contains 1 pair of chromosome D from R.

用普通小麦-纤毛鹅观草双倍体与普通小麦中国春连续回交两次,然后连续自交,通过形态观察、根尖细胞染色体计数、花粉母细胞减数分裂中期Ⅰ染色体配对分析及染色体C-分带,在BC2F3和BC2F3群体中,分别筛选到一个端二体异附加系94K227和一个二体异附加系94K280,其中C-分带显示94K227添加的是纤毛鹅观草染色体B的一对长臂, 94K280添加的是纤毛鹅观草的一对染色体D。

In order to analyze the conservation of maize centromeric satellite DNA and centromeric retrotransposon in the subspecies and relatives of Zea mays, dual fluorescence in situ hybridization was used to detect the existence and distribution of the above two repetitive sequences in Zea mays ssp. mexicana, Z. diploperennis, Z. perennis, Tripsacum dactyloides, Coix lacryma-jobi, and Sorghum bicolor.

为分析玉米着丝粒卫星DNA和着丝粒反转录转座子在玉米种的亚种及近缘种中的保守性,采用双色荧光原位杂交检测了这两种重复序列在墨西哥玉米、二倍体多年生类玉米、多年生类玉米、摩擦禾、薏苡、高粱中的存在和分布。

Employing high-dilute method and 5-fold excess amine prepared two new "two-armed" acyclic diamine intermediates I_a and I_b, which are also intermediates of synthesizing microcyclic compounds, and eight aromatic Schiff bases, II_, were synthesized from reaction of I_a or I_b condensing with different aromatic aldehydes. Their composition and structure were characterized by Elemental analysis, IR, UV-Vis, LIF,~1HNMR and MS. The hydrogen bond interactions and MS fragmental patterns were also discussed.3. Several novel transition metal complexes using II_a as ligand were synthesized and identified by Elemental analysis, IR, UV-Vis,~1HNMR and molar conductivity.

1,采用高度稀释及胺过量五倍的方法合成了两个具有吡啶双酰胺臂的非环二胺Ⅰ_a和Ⅰ_b,并以它们为中间体进一步合成了八个芳香席夫碱Ⅱ_a-Ⅱ_h,通过元素分析及各种波谱测试鉴定了它们的组成与结构,对化合物的UV-Vis、LIF、IR、~1HNMR及MS进行了较为详细的讨论,还探讨了席夫碱的氢键相互作用及质谱裂解方式; 2,以席夫碱Ⅱ_a为配体合成了几个新型的过渡金属配合物,并通过元素分析、UV-Vis、IR、~1HNMR、摩尔电导及DTA—TG对其进行了表征,讨论了化合物的配位情况及配体在配位中的脱质子行为。

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