双二倍体

Wheat germplasm lines were obtained from the posterity of Aegilops tauschii-Triticum Carthlicum amphiploid, and analysis results showed that the 30 germplasm lines have many excellent characters, such as multi-tillers, multi-spikes per plant, good disease resistance, large ear, etc., they are very useful in genetic improvemert of wheat.

从粗山羊草与硬粒小麦合成的双二倍体后代中选育出30个种质系，对其鉴定结果表明，它们的分蘖力、单株穗数、抗病性、穗长等性状表现优异，具有重要利用价值。

Having a diploid set of chromosomes derived from each parent.

双二倍体的具有从父母''。'双亲'。''获得的两套染色体的

Fig 1 The results of two color FISH A:Normal sperms(a sperm nucleus with a red fluorescence domain was X sperm, and with a green fluorescence domian was Y sperm);B:Numeric chromosome abnormal sperm two green fluorescence domains within the same sperm nucleus which may be classified in Y disomic sperm or diploidy sperm, in the case of hybridization with two probes, one euchromosome specific and the other sex chromosome specific, this sperm could be discriminated as disomic or diploidy sperm

图1 双色荧光原位杂交结果 A：正常精子(精子头部有1个红色荧光信号者为X精子，有1个绿色荧光信号者为Y精子)；B：染色体数目异常精子两个绿色荧光信号同在1个精子核内，可为Y双体精子或二倍体精子，若用1条常染色体探针和1条性染色体探针进行双色荧光原位杂交，可以区别该精子属Y双体精子或二倍体精子

If 〓mRNA positive expression, its DNA heteroploid type display as triploid or hypodiploidy, if 〓mRNA negative expression, its DNA heteroploid type display as hyperdiploidy、tetraploid or Bistem line. These from one flank demonstrate the role of 〓 gene suppress the proliferation of malignant tumor

表达阳性者，其DNA异倍体主要表现为三倍体或低二倍体，〓表达阴性者，其DNA异倍体主要表现为高倍体、四倍体和双干系，从一个侧面显示出〓基因的抑制恶性肿瘤细胞增殖的作用。

There was big difference among the 22 different materials in the disease resistance. Translocation lines Pm97033 showed the highest resistance in reaction than others. The percentage of diseased root and severed level were 11.3% and 5.4%; substitution lines Wan7107 showed medium resistance to the fungus, the percentage of diseased root and severity level were 21.4% and 10.6%; F5 generation of common wheat with Till showed higher resistance to take-all fungus than the PS progenies of common wheat with Aegilops tauschii or the other common wheat varieties.

菌饼＋菌粒法对宁春4号的致病力明显高于菌饼法和菌粒法，其病根率、严重度和病茎率分别达到100.0%，57.3%和28.6%。22个遗传背景不同的小麦材料间抗病性差异较大，其中小麦－簇毛麦易位系Pm97033的抗病性最强，病根率和严重度分别为11.3%和5.4%；代换系Wan7107次之，病根率和严重度分别为21.4%和10.6%；硬粒小麦－簇毛麦双二倍体(TH1)与普通小麦品种杂交后代的抗病性强于粗山羊草与普通小麦品种杂交后代。

DNA C-values might be one of important indices in the evaluation of angiospermous invasiveness. To test this viewpoint, we compared DNA 1C-values and basic genome sizes in 3,676 angiosperms. The results suggest that:(1) the two nuclear values vary greatly among different groups, e.g., significantly higher in herbs than in trees, in monocots than in dicots, in perennials than in non-perennials;(2) the two nuclear values both have significant effects on plant invasiveness, especially in herbs, dicots, monocots, perennials, non-perennials, diploids, polyploids, Compositae, and Poaceae, while not significant in trees and Fabaceae.

DNA C值与被子植物入侵性密切相关，为考察应用DNA C值评估被子植物入侵性的可靠性和局限性，我们统计分析了全世界范围内3676种被子植物的DNA 1C值（配子未发生DNA复制时的染色体DNA含量）及基因组大小（单个染色体组的DNA 含量），结果表明：1）DNA 1C值和基因组大小在被子植物不同类群中差异极大，例如在草本植物中显著高于木本植物，单子叶植物显著高于双子叶植物，多年生植物显著高于非多年生植物；2）两项指标都与被子植物入侵性显著负相关，尤其是在草本植物、双子叶植物、单子叶植物、多年生植物、非多年生植物、二倍体、多倍体、菊科和禾本科中，然而在木本植物、豆科植物中其与植物入侵性无显著关系。

In the studies on transferring and identifying alien chro-mosomes into wheat,the combined use of mitotic and meioticchromosome N-banding is also successfully practiced in deter-mination of T.durum-H.villosa amphipoid,five wheatalien disomic additions with H.villosa chromosomes,〓〓,alien sulstitution line with chromosome 〓 andsome translocation lines.

在外源染色体导入、鉴定研究中，利用根尖有丝分裂中期染色体和花粉母细胞减数分裂中期Ⅰ染色体的N-分带，快速、准确地鉴定了硬粒小麦一簇毛麦双二倍体、普通小麦一簇毛麦异耐加系V〓、V〓、V〓、V〓和V〓，普通小麦一簇毛麦异代换系V〓以及一些具有易位的杂种后代。

In this study,Amplified fragment length polymorphism analysis was carried out by using 23 pairs of EcoRI-NN/MseI-NNN selective primers in amplification of the DNAs from reciprocal F_1 hybrids of Cucumis hystrix Chakr.and C.

为了研究细胞质背景对基因组序列丢失的影响，利用23对Eco RI-NN/Mse I-NNN选择性引物对Cucumishystrix与栽培黄瓜C.sativus的正反交F_1、异源双二倍体及亲本DNA进行AFLP分析。

The behavior of chromosomes at meiosis of PMC of 9 combination and selfed seed set of 16 combination of Triticum-Aegilopsamphidiploids were studied.

对小麦-山羊双二倍体9个组合的细胞遗传学和16个组合的自交结实率进行了观察。

A wheat- Th.intermedium addition line,which was yellow rust resistance,was found to be homoeologous to group 7 based on endopeptidase isozyme analyses.

对小麦—中间偃麦草部分双二倍体无芒中 4、异附加系 C0 76、宛 71 0 7和中国春进行了肽链内切酶(EP- 1 )等电聚焦电泳，结果表明，肽链内切酶在阳极处有一特异带。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。