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双二倍体

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The genomic changes occurred form F_1 and the greatest changes of sequence was occurred from S_0 generation to S_1 generation,while,there were miner changes from S_1 to S_2, which indicated that the allopolyploid genome became more stable following the selfing process and the sequence change was a rapid event.

但在S_1到S_2代基因组序列变化比率基本接近,暗示了伴随着异源双二倍体的自交过程,早期基因组表现出稳定的趋势,说明基因组序列变化是一个快速的事件。

The result showed that newwheat species might be prodcued in progeny of unstable amphidiploid, which offeredsignificant pattern for evolution from octaploid wheat to hexaploid wheat and widenedthe train of thought of studying the origin of biological species.

这个结果显示,通过对双二倍体分离后代的有效选择,有可能形成一个新的小麦种,从而为从八倍体小麦向六倍体小麦演化提供了一个新的进化模式,拓宽了研究物种起源的思路。

Studies showed that the Giemsa-C banding patterns of Ae.tauschii- H.vulgare amphidiploid is much in accord with that of its two parents, and this further proved that the regenerated plants from embryo callus of hybrids between Ae.tauschii and H.vulgare (2n=28) were spontaneously chromosal doubling amphidiploid.

分析表明,节节麦-大麦双二倍体Giemsa--C带核型与两个亲本的带型基本一致,这证明了节节麦-大麦双二倍体的染色体组成。

Using SDS-PAGE technique, HMW-GS of Thinopyrum were detected by analyzing Thinopyrum accessions, wheat-Th. elongatum 1E〓 substitution lines and wheat-Th. intermediam partial amphidiploid Zhong2. It was showed that the composition of HMW-GSs of Thinopyrum were distinctly different from which in wheat.

对偃麦草、二倍体长穗偃麦草1E中国春代换系、小麦—中间偃麦草部分双二倍体中〓高分子量谷蛋白亚基进行了SDS-PAGE分析,发现在偃麦草材料中存在不同于小麦的特异HMW-GS类型。

By Giemsa--C technique, the chromosomes of Aegilops tauschii (85A-34) and Hordeum vulgare (H2) and Ae.tauschii- H.vulgare amphidiploid were all produced clear bands, the terminal band, centromeric band, secondary constrition band and rich intercalary bands were displayed distinctly, thus the corresponding Giemsa-C banding patterns were confirmed.

利用Giemsa--C带显带技术,节节麦、大麦、节节麦-大麦双二倍体都显示出了清晰的带纹,都具有端带、着丝点带、随体带和丰富的中间带,相应的确定了它们的Giemsa--C带核型。

Studies on sequence changes in the early genomes of interspecific F_1 hybrid and amphidiploid in CucumisIn the present study,extensive genomic changes were detected in newly obtained allotetraploid of Cucumis,namely Cucumis hytivus Chen and Kirkbride,by AFLP analysis using 12 pairs of EcoR-I/Mse-I primers.

甜瓜属远缘杂种F_1及人工异源双二倍体早期基因组序列变化研究利用12对Eco RI/Mse I选择性引物,对甜瓜属人工异源双二倍体C.hytivus及其二倍体父母本进行AFLP分析,结果发现多倍体基因组在形成早期发生了广泛的序列变化。

Common wheat ; Wheatgrass ; Amphiploid ; Specific DNA sequences ; RFLP analysis ; Genomic changes ; Polyploid genome evolution

小麦;天蓝冰草;双二倍体;专化DNA序列; RFLP分析;基因组变异;多倍体基因组进化

GISH technique was applied to TAF46 a partial amphiploid (2n=8x=56) derived from a Thinopyrum intermedium×Triticum aestivum hybrid and to 6 of the possible disomic addition line derived from it to determine the genome origin of the alien genome and the addition lines.

应用GISH对小偃麦部分双二倍体TAF46(2n=8x=56)及其衍生的6个二体异附加系的中间偃麦草染色体组种类进行了分析、鉴定。

The result suggested that different genomes had different genetic speed or stability during evolution.3 The similarities co-efficiency between wild emmer and hexaploid synthetic was higher than that between wild emmer and common wheat. So that crossing among species is not a simple combination of genomes, but involve introgression of chromosomes, and the evolution made by this action was bigger than that made by environment.4 According to the results of salt tolerance identification, the salt tolerance of wild emmer is lower than that of common wheat in the whole.

野生二粒小麦与普通小麦之间的遗传相似系数稍高于野生二粒小麦与其与节节麦合成的双二倍体的遗传相似系数,说明物种之间的杂交并不仅仅是染色体组简单的合并,其中包括复杂的染色体组本身的一些特性以及染色体之间的互渗且这种作用远大于环境作用所引起的变化。

However, the use of Aegliops tauschii in improvement of wheat yield trait has not been reported. In this study, T.durum-Ae.Squarrosa amphidiploids was crossed with common wheat PH85-16, then backcrossed with PH85-16. The variation characteristic of the main yield traits of BC1F1 and BC1F2 were analyzed. Then, QTLs affecting yield traits were detected by 130 SSR primer pairs of D genome in BC_1F_1 and BC_1F_2.The result showed chromosomes in D genome from Aegliops tauschii affected yield trait of wheat significantly.

本研究以二倍体小麦粗山羊草与四倍体硬粒小麦合成的双二倍体Am6为亲本,与普通小麦PH85-16 进行杂交,再用PH85-16 进行回交,对获得的回交后代进行了主要产量性状调查,利用位于小麦D 基组的SSR 分子标记进行了分子标记分析,对来自粗山羊草与产量性状相关的数量性状位点进行定位,探讨粗山羊草对小麦产量性状的影响。

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