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The effects of several components in the culture medium on Lithospermum erythrorhizon cell growth and secondary metabolite synthesis were studied, as well as structural dynamic model. The two-liquid-phase culture of Lithospermum erythrorhizon was carried out by choosing the proper organic solvent as the second phase. The bioactive carrier for adsorption was prepared and the condition of cell immobilization was determined. We combined the technique of two-liquid-phase culture and immobilization to carry out the culture. We chose the suitable type of reactor, studied its characteristics and results of cell culture using this reactor. The fed-batch operation was also studied on the basis of twoliquid-phase culture and immobilization used in culture in the reactor.

本文研究了紫草细胞悬浮培养中培养基中多种成分对细胞生长与次生代谢产物合成的影响,进行了结构化的动力学模型研究;通过选择合适的有机溶剂对紫草细胞进行了液相培养研究;通过确定以吸附为细胞的固定化方法,进行了生物活性吸附载体的制备与固定化细胞的制备研究;并结合液相培养技术,对紫草细胞进行了固定化培养及其动力学模型的研究;对反应器进行选型,并进行冷模与热模研究;在反应器中进行了固定化紫草细胞的液相培养条件下的流加操作研究。

Results There were hemodynamic changes of double kidneys in all 120 cases of pregnant women, thereinto, the signs were seen for nephrauxe, hydronephrosis (including 35 cases with hydrops of double kidneys, 12 cases with hydrops of left kidneys ) and sparate collecting system of kidney in various degree in 108 cases, otherwise, there were no visible ultrasonic symptom on hydrops of double kidneys, but low-grade nephrauxe in 12 cases.

结果 120例中孕妇侧肾脏血流动力学均发生改变,108例受检孕妇中均有不同程度的肾脏体积增大、肾脏集合系统分离、肾脏积液,其中35例侧肾脏积液,12例仅表现为左侧肾脏积液;120例中有12例侧肾脏未见明显积液声像,但有轻度的肾脏体积增大。

To get more insight into the mechanism and kinetics of the RAFT process,it would be helpful to build a small molecular model reaction mimicking the RAFT process but free of a chain-propagation event. We hereby propose a model cross reaction between dithioester and alkoxyamine as a probe of the RAFT process,in which the alkoxyamine plays the role of the radical source.The generating rate of the transient alkyl radical and the nitroxyl radical can be described by the law of persistent radical effect.Thus,the advantage of the model cross reaction is that it affords a radical concentration determined by the known equation of the persistent radical effect.

为了更深入的理解RAFT聚合过程,我们构建了一个模型反应:即由烷氧基胺与硫酯进行交换反应,烷氧基胺均裂产生的烷基自由基将会向硫酯进行链转移,硫酯的引入对纯烷氧基胺分解动力学造成的影响可以提供RAFT过程的许多信息。

Upon binding ATP or a nonhydrolyzable analog such as AMPPNP, RecA undergoes an allosteric transition into a high-affinity DNA-binding form. When RecA combines with dsDNA to form RecA-dsDNA nucleofiliment, it can unwind dsDNA and change the dsDNA structure.

在ATP或其类似物的辅助下,RecA可以直接与股DNA结合而形成核丝,并将股DNA加以解螺旋,并改变股DNA的结构。

It is shown that because of the high nonlinear of oil film force of SFD, the nonsynchronous response and the bistable operation may occur in the rotor-SFD system, especially the latter with high vibration amplitude, which has a great effect on the reliability of the rotor system.

结果说明了由油膜力的高度非线性所引起的非协调及稳态,特别是当转子需要频繁的通过稳态区时,稳态跳跃将使转子系统的振动迅速增大,在实际应用中应对其引起足够的重视。

Finally, the cytotoxic and antibacterial activities of the oxamido-bridged binuclear complexes were studied in vitro.. This dissertation consists of three sections as follows:1. The crystal structure of a oxamide dinitrate,(1) [H_4dmaeoxd](NO_3)_2 H_2dmaeoxd = N,N\'-bis[2-ethyloxamide, has been obtained. The diversity of complexes with different structures is carried out by successful synthetic strategy of controlling species of metal ions, counter anions, pH values and solvents in the course of synthesis. Four dinuclear complexes bridged by trans-dmaeoxd2-: [Cu_2_2](ClO_4)_2 (2), [Cu_2_2](ClO_4)_2 (3),、[Cu_2(Me_2phen)_2](ClO_4)_2 (4), [Cu_2_2(H_2O)_2] (5); Four dinuclear complexes bridged by cis-dmaeoxd2-: [NiNi_2](ClO_4)_2 (6), [CuNi_2](ClO_4)_2·2CH_3OH (7), [CuZn_2]-(ClO_4)_2·2H_2O (8), [Cu(H_2O)Ni_2](ClO_4)_2.0.5CH_3OH (9); three 1-D polynuclear complexes: [Cu_2]_n·nH_2O (10), [Cu_2(H_2O)_2-]_n·4nH_2O (11), [Cu_2(H_2O)]_n(NO_3)_n·2nH_2O (12); one 2-D polynuclear complex,[Cu_6_3(μ_3-OH)_2(H_2O)_2]_n(ClO_4)_(4n).2nH_2O (13); the others: [VO(H_2O)]·2H_2O (14), [Zn_2_4](ClO_4)_2·H_2O (15), [Cu_2]·2DCM (16) have been synthesized and characterized by X-ray single crystal diffraction, elemental analysis, IR spectra.

本论文主要包括以下三部分:一、合成得到了一个草酰胺配体硝酸盐的单晶结构,[H_4dmaeoxd](NO_3)_2 (1);通过调控金属离子的种类、端基配体、抗衡阴离子、溶剂、pH值等条件定向合成了四个反式草酰胺桥联核铜配合物:[Cu_2_2](ClO_4)_2 (2)、[Cu_2_2](ClO_4)_2 (3)、、[Cu_2(Me_2phen)_2](ClO_4)_2 (4)、[Cu_2_2(H_2O)_2] (5);四个顺式草酰胺桥联同/异核配合物:[NiNi_2](ClO_4)_2 (6)、[CuNi_2](ClO_4)_2·2CH_3OH (7)、[CuZn_2]-(ClO_4)_2·2H_2O (8)、[Cu(H_2O)Ni_2](ClO_4)_2.0.5CH_3OH (9);三个一维聚合物: [Cu_2]_n·nH_2O (10)、[Cu_2(H_2O)_2-]_n·4nH_2O (11)、[Cu_2(H_2O)]_n(NO_3)_n·2nH_2O (12);一个二维聚合物: [Cu_6_3(μ_3-OH)_2(H_2O)_2]_n(ClO_4)_(4n)。2nH_2O (13);单核钒配合物[VO(H_2O)]·2H_2O (14)、草酸根桥联核锌配合物[Zn_2_4](ClO_4)_2·H_2O (15)、美洛西康铜配合物[Cu_2]·2DCM (16),利用元素分析、摩尔电导、红外光谱、电子光谱和单晶X-射线衍射对上述所得化合物进行了结构表征,探讨了结构影响因素,并研究了其超分子结构。

The test result shows that the mechanical properties of the dual-phase structure are much higher than those of ferritic plus pearlitic structures, that the dual-phase structure is of better corrosion resistance in some cases for Ca_2 has corrosion-moderating effects, and that hot-rolling dual-phase structure of the low-carbon Mn-Si steel wit...

试验结果表明:相组织的力学性能远高于通常的铁索体+珠光体组织;在一定条件下,相组织具有更好的耐蚀性,Ca_2有明显的缓蚀作用;采用台阶淬火工艺,不含Cr、Mo的低碳锰硅钢也易于获得热轧相组织。

Sulfonic-4-(β-sulphatoethyl-sulphone) phenylamine is often applied to producing bifunctional-soluble reactive dyes and is an important dye intermediate. It plays an important role in the synthesis of KN style reactive dye M style double reactive dye and modified dissimilar double reactive dye. Used 2-sulfonic-4-(β-sulphatoethyl-sulphone) phenylamine as raw material can improve the uniformly dying performance and scourablility of dyestuff molecules and can improve the firm degree of colour staining.

2-磺酸-4-苯胺是制备水溶性活性染料的重要中间体,在KN型活性染料、M型活性染料以及改性异种活性染料的合成中起着重要的作用,用2-磺酸-4-苯胺作原料合成活性染料可增加染料分子的匀染性和易洗涤性,提高染料的粘色牢度。

A series of novel polys: poly, poly and their copolymer poly were synthesized at high temperature by nucleophilic polycondensation of bisphenol 4 (3 phenyl 4 hydroxyphenyl) 2,3 phthalazin 1 one with activated difluorodiphenylketone, dichlorodiphenylsulfone and both together.

将苯基取代类酚化合物 4-(3 -苯基-4-羟基苯基)-2 ,3 -二氮杂萘-1 -酮与活性卤单体如 4,4 -二氟二苯酮、4,4 -二氯二苯砜进行高温缩聚反应,合成了高分子量的新型苯基取代杂萘联苯型聚芳醚材料;用1 H NMR、FT-IR表征了类酚单体及聚合物的结构。

But under the same conditions, the reactions of the complex (41) with organic lithium reagents give a kind of novel complexes in which the nucleophile is added to the a-carbon of the phthalazine ring.

然而含有桥羰基的2,3-二氮杂萘配位的羰基铁络合物4l在相同的条件下与芳基锂试剂反应时,芳基锂却是亲核加成在氮杂环的α位羰上,生成一类结构独特的铁羰基络合物。

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What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢?

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我,一个心中布满虚荣的女孩子真实的想法。

Oh, and I bought myself a new laptop," he said."

哦,我还给我自己买了一个新笔记本"他说。"