参考系统

After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象，提取细菌基因组DNA，用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切，回收大小为1～5Kb的DNA片段，将其连接入原核表达载体pET-28a-c，最后转化宿主菌，结果成功地构建了基因表达文库，为后续的基因筛选工作奠定基础；另外，本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计，以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板，进行PCR扩增反应，结果表明成功地获得两个新基因：23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸)，并进一步做了不同物种之间的分子系统发育分析，构建了基于23S rRNA基因的邻接法系统发育树，阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

Third, introducing the evaluation theory of ecosystem service, it set up an appraisable system of ecosystem service values of water resources, which is specially carried on to Haihe Basin too. Fourth, it coupled the evaluation theory of ecological service function and the holistic analysis model of water resources carrying capacity for a try, and then set up the analysis mechanism of regional macro-economy, water resources and ecological environment, which carried on a preliminary research of carrying capacity of ecological environment of water resources in Haihe Basin.

第二，建立了水资源承载能力分析模型，针对海河流域进行专门的分析，提出流域合理的经济社会发展规划参考目标，第三，引入生态系统服务功能价值理论，建立了水资源生态系统服务功能价值评价体系，并在海河流域内进行实践应用，第四，将生态服务功能价值理念与水资源承载能力分析模型进行尝试性的耦合，初步提出基于区域宏观经济、水资源及生态环境分析机制的流域的水资源生态环境承载能力分析框架。

By studying characteristics, merits and solving problems of the different nozzle and new spray equipment in asphalt gas decontaminating for carbon calciner, the research provides a basis for the industrial design of swirl nozzle.

在阐述沥青烟气净化系统中不同雾化设备及新型雾化设备特性的基础上，分析了碳素焙烧炉沥青烟气净化系统的雾化冷却的技术现状，并指出了存在的问题，为工程设计选择雾化设备提供了参考

In order to help people have a better understanding on third-party logistics, which to make it play a more effective role, thus is better for the economic development and the improvement of people's life. This paper based on a rapid development logistics companies --Lang Chaco logistics , and analyze the company's logistics structure effectively in order to find its shortcomings, also, design a structure more complete logistics system for reference.

为了使人们更进一步的认识和了解第三方物流，以使其更有效的发挥自身的作用，从而更好为经济的发展和人们生活水平的提高服务，本文立足于一个处于快速发展的物流公司---浪潮物流，对其业务的处理流程和整个系统的高效性进行了分析，力求在此基础上找出其不足，设计一种结构比较完善的物流信息系统，以供参考。

In order to help people have a better understanding on third-party logistics, which to make it play a more effective role, thus is better for the economic development and the improvement of people's life. This paper based on a rapid development logistics companies --Lang Chaco logistics , and analyze the company's logistics structure effectively in order to find its shortcomings, also, design a structure more complete logistics system for reference.

为了使人们更进一步的认识和了解第三方物流，以使其更有效的发挥自身的作用，从而更好为经济的发展和人们生活水平的提高服务，本文立足于一个处于快速发展的物流公司---青海省邮政局物流，对其业务的处理流程和整个系统的高效性进行了分析，力求在此基础上找出其不足，设计一种结构比较完善的物流信息系统，以供参考。

As the method of summary classific ation does not fit with automatic summarization classification,automatic summar ization is classified using different standards in the paper. The classification is based on the features of automatic summarization.

目前 ，文摘的分类方法不适用于自动文摘的分类，因此，本文试着从多角度对自动文摘系统进行了分类，这样的分类根据自动文摘的特点进行的划分，是对自动文摘分类的一种总结，可以作为构造自动文摘系统和思考自动文摘发展方向的参考和借鉴。

In order to promote the protection and utilization of mangrove ecosystem,this paper reviewed some ecological functions of mangrove ecosystems which can provide large quantity of organic matters,epeirogenic processes and shelterbelts,and protect dikes...

本文对红树林生态系统在生产有机物、维持热带亚热带河口生态系统、降低风速以及海水流速、防治和减轻灾难、截留碎屑形成陆地、保护海堤、吸附污染物净化水体、提供多种生境、维护生物多样性，以及形成优美的环境开展生态旅游等几个方面的生态服务功能进行了综述，旨在为红树林资源的保护与合理利用提供参考。

Water-stage and discharge immediate information for hydraulician in decision-making time important basis, after water conservation bureau many years endeavor, in this bureau website, has nowadays been possible to provide inquiry the immediate important river water-stage unconfirmed value, but this water-stage the relative discharge firmly still was unable to know, this research namely based on this consideration, was the application network technology conformity information bank management system management system, the organic set of water-stage and discharge conversion system, the time could provide reference current discharge inquiry this immediate water-stage.

水位与流量即时资讯为水利工程师在决策时之重要依据，经水利署多年之努力，现今该署网站上，已可提供即时之重要河川水位未经确认值之查询，但该水位之相对流量确仍无法得知，本研究即基於此考量，乃应用网路技术整合资料库管理系统，建制一套水位流量换算系统，期能提供该即时水位之参考流量查询。

This model has overcome the shortcoming of single CBP model and other models limited in ability to express with double trend prediction; also separated the time dependence and environmental factor, and respectively treated the two weight of the double trend, this model have fully utilized the advantage of the CBP isotropism characteristic.

最后，以云南某烟草公司某区域近6年的卷烟销售数据为研究模型试验研究对象，建立了APMSTS基于神经网络的烟草销售预测模型系统，系统从该公司数据库中进行实时增量抽取数据，以及录入该区域年鉴数据作为环境参考因子。

The serious recording limitations due to the finite size of CCD and the space-bandwith products of an off-axis system and a lensless Fourier transform system are discussed.

分析了CCD参数对记录条件的限制、不同参考光的记录对CCD空间带宽的影响以及普通离轴全息系统与无透镜傅里叶变换全息系统中信息量的空间带宽积问题。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。