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Result The nested PCR assay was specific and there is no cross-reaction with other parasites, such as Eimeria spp., Cryptosporidium spp., Giardia sp., Toxoplasma sp., Trichuris sp. and cattle ciliate. The assay was able to detect as low as 2.85 × 10^(-2) fg of control positive DNA. The results of detection of clinical samples revealed that the assay was coinciding with microscopic examination.

该套式PCR能特异性地扩增出牛源环孢子虫基因组DNA中相应片段,与艾美耳球虫、隐孢子虫、贾第虫等10种相关原虫基因组DNA无交叉反应;最低能检测到2.85×10^(-2)fg的阳性参照DNA;对临床样品检测结果表明PCR技术检查阳性者显微镜检查都为阳性。

Article 57 Where the valuation or the receipt or payment of usage fees in respect of such business dealings as the transfer of property or the granting of rights to the use of property between an enterprise and an associated enterprise is not based on business dealings between independent enterprises, adjustments may be made thereto by the local tax authorities with reference to amounts that would be agreed to by unassociated and unrelated parties.

第五十七条企业与关联企业之间转让财产、提供财产使用权等业务往来,不按独立企业之间业务往来作价或者收取、支付使用费的,当地税务机关可以参照没有关联关系所能同意的数额进行调整。

In the case in which the alienation of property or the provision of property rights between an enterprise and its associated enterprise is not priced, charged or paid according to the rates for business dealings between unassociated enterpris , the tax authorities may make adjustments with reference to the amount that may be agreeable between unassociated parties.

第五十七条企业与关联企业之间转让财产、提供财产使用权等业务往来,不按独立企业之间业务往来作价或者收取、支付使用费的,当地税务机关可以参照没有关联关系所能同意的数额进行调整。

Use properly placed, properly made matched HUNTING arrows (matched to the bow/archer combination, and made for hunting; hunting arrows should be about 10 grains for every pound of draw, sometimes heavier - 400 grains for a 40 lb bow is a good starting point), with SHARP broadheads, and you won't have to worry about being "underbowed"-- as long as you pay to the local regulations where you hunt.

只要能准确的打中有效部位,用猎箭(每磅至少10格令,对初学者来说40磅的配400格令挺好的),用极锋利的猎箭头,你不用担心威力不足--参照各州法律规定在什么地方狩猎(译者说明,一般必须上树)。

In this study, We used longer DD primers in combination with a two-step PCR protocol to overcome these two problems in term of modified methods of Martin and Pardee.K562 erythroleukemia cell differentiation induced by HEMIN or PMA for 36 hours was used as erythriod or megakaryocytic differentiation model in vitro.Total RNAs were extracted from induced and uninduced K562 cells and were applied to mRNA differential display analysis.

为解决此问题,我们参照Martin和Pardee的改进方案[3],建立了一种使用长引物,进行二步PCR的改进DDRT-PCR方法,以氯高铁血红素和肉豆蔻佛波酯诱导K562细胞36小时作为体外红细胞和巨核细胞分化模型,取诱导前后的细胞总RNA进行mRNA差异显示分析。

In allusion to the current situation that the administrative reconsideration law is implemented unsatisfiedly in China, it is necessary to set the principle of adverse alteration prohibition in administrative reconsideration procedure in accordance with the judicial remedy procedure and alien administrative remedy practice. The principle has its special meaning, character and applying rules. It doesn't disobey the legal principle of current constitution and governmental organic law and the principle o...

针对目前我国行政复议法实施不尽人意的现状,有必要参照司法救济程序和域外的行政救济实践,在我国行政复议程序中确立不利变更禁止原则;行政复议中的不利变更禁止原则有其特定的含义、特征和适用规则;在行政复议程序中确立不利变更禁止原则并不违背现行宪法和政府组织法的立法宗旨,也不会冲击依法行政原则,相反,却有利于保护行政相对人的复议申请权和充分发挥行政复议制度的功能。

9 With reference to Exception No.1 to 11.2.8 , the magnet coil of a molded bobbin transformer having a slot for the crossover or start lead - unspliced at the windings - is acceptable as crossover lead insulation if :"

& 11.2.9 参照11.2.8的例外1,胶芯的磁铁线圈的变压器的交叉或开头引线有一个狭缝——在绕组处没有连接——作交叉引线绝缘是可接受的,如果:&

The current unstressed configuration is adopted as reference in global analysis, which is better to solve the problem of accumulated large displacement and large rotation during collapse.

本文采用当前无应力构形作为参照建立总体方程,较好的解决了结构倒塌过程中的累积大位移和大转动问题。

Closed system , sealed system , unvented system

参照开式系统9.14。

According to morphology of the telispore and telispore balls, the pathogen was identified as Urocystis dioscoreae Syd.

根据引起病害的症状特点,参照相关文献将其鉴定为山药条黑粉菌(Urocystis dioscoreae Syd。·)。

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Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在,'歼灭战perfuming自己与胡努特,那麼,他来到和SAT 。

The shamrock is the symbol of Ireland and of St.

三叶草是爱尔兰和圣特里克节的标志同时它的寓意是带来幸运。3片心形叶子围绕着一根断茎,深绿色。