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METHODS AND MATERIALS We collected mosquitofish from a pond in Mecklenburg County, North Carolina, to condition water with predator chemicals.

材料与方法我们收集mosquitofish ，从一个池塘中mecklenburg 县，北卡罗来纳州，以水条件与捕食者的化学品。

Falciparum isolates prepared directly from 5 cases of cerebral malaria patients'blood in Mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding the regions 12—17 in MAD20 merozoite surface protein 1(MSP1) of Papua New Guinea strain of P . falciparum were used as primers. The PCR products were digested with EcoRI and KpnI, respectively, and the generated fragment regions 16—17 were cloned into M13mp18 and M13mp19 vectors and their DNA were analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应技术对中国5 例脑型疟患者恶性疟原虫云南省勐腊县勐罕CMH/YN 分离株和云南省盈江县农场CYJ/YN 分离株基因组DNA 裂殖子表面蛋白1 (MSP1)第13—17 区基因进行扩增，将扩增产物分别经EcoRI 和KpnI 双酶切后，回收的MSP1 第16—17 区基因分子定向克隆M13mp18 和M13mp19 载体，按Sanger 双脱氧链终止法进行DNA 序列测定，并与MAD20、K1 和Wellcome 株原型基因进行同源性分析比较。

Methods Genomic DNA samples of two isolated Plasmodium falciparum isolate strains prepared directly from 5 cases of cerebral malaria patients′ blood in mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding FC27 merozoite surface protein 2 (MSP2) of Papua New Guinea strain of Plasmodium falciparum were used as primers. The PCR products were digested with BamH1 and Hind Ⅲ respectively, and the generated fragment MSP2 were cloned into M13mp18 and M13mp19 vectors and their DNA was analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

应用多聚酶链反应对5例中国脑型疟患者恶性疟原虫云南省勐腊县勐罕分离株和云南省盈江县农场分离株基因组裂殖子表面蛋白2(MSP2)基因进行扩增，将扩增产物分别经BamHI和Hind Ⅲ双酶切后，回收的MSP2基因分子定向克隆M13mp18和M13mp19载体，按Sanger双脱氧链终止法进行DNA序列测定，并与恶性疟原虫株FC27、K1、IC1和CAMP株进行同源性分析比较。

Results In 1980, the filariasis have been basic eliminated in Yangshan County, the microfilaria rate have been come down to 0.120% from 4.35% before control.

结果经过反复查治，阳山县微丝蚴率从防治前的4.35％降至0.12％，1980年达到基本消除丝虫病标准，1996年经广东省卫生厅组织评审，确认全县范围已阻断丝虫病传播，达到了消除丝虫病标准。

The quake hit Thursday morning off the coast of Miyagi, about 180 miles (290 kilometers) north of Tokyo, the agency said.

气象部门表示，地震发生在东京以北大约180英里（290千米）的宫城县海域上。

The splendor of the coastline as a whole has makes it one of Miyagi's top spots for beauty.

沿岸一带海岸线优美、曲折，作为宫城县有代表性的风景区颇具名声。

Tomo was born in the former town of Hasama, in Miyagi Prefecture.

大友克洋出生于日本宫城县Hasama的小镇。

In 1265, Simon de Montfort summoned the Great Council to meet at Westminster, together with 2 knights from each county and 2 citizens from each town, a meeting which has been seen as the earliest parliament.

1265年，西蒙·德·孟弗特在威斯敏斯特召集大议会，每个县两名骑士、每个镇两名市民参加。这次会议被称为是最早的议会。

SHENGYUAN hotel is first four star hotel in ZIJIANG county,the decoration is eastern conventional style,it's elegance unique,harmonious morbidezza and uncommon style.

本酒店是资源县首家四星级商务酒店，其装饰为东方传统的中式风格，尽显高雅别致、和谐柔美、气派非凡。

Diet composition of forest musk deer Moschus berezovakii in winter and spring was studied by using a method of micro histological analysis of feces.

利用粪便显微组织分析法对陕西凤县野生林麝冬季和春季的食性进行了研究。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。