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From the femur bone density analysis of protein expression, we can see that light chain lactoferrin, annexin decreased and the expression of enolase, ATP synthase, acetyl-coenzyme A reductase, the expression of troponin, may be related to ovariectomized femoral bone density in osteoporosis-related happened; strong bone of the femoral osteoporosis Po intervention effect may be related to reduced enolase, ATP synthase, troponin, CK-MB, acetyl phosphoglycerate enzyme, the expression of myosin, and the increase in lactoferrin light chain, pyruvate kinase isoenzyme, protein expression crown.

从股骨密质骨蛋白质组表达分析可知,乳铁蛋白轻链、膜联蛋白A3的表达下降及烯醇化酶、ATP合酶、乙酰辅酶A还原酶、肌钙蛋白的表达增强,可能与去卵巢后股骨密质骨骨质疏松的发生相关;强骨宝对股骨骨质疏松的干预效应可能与下调烯醇化酶、ATP合酶、肌钙蛋白、肌酸激酶同工酶、磷酸甘油酸变味酶、肌球蛋白的表达,和上调乳铁蛋白轻链、丙酮酸激酶同工酶、冠蛋白的表达有关。

Cancellous bone from the lumbar proteomic expression analysis show that actin, keratin, enolase, ATP synthase, the expression of myosin may be related to ovarian lumbar cancellous bone after osteoporosis-related happened; Qiang Gu Bao Po on the lumbar spine osteoporosis intervention effect may be related to reduced carbonic anhydrase, actin, crystal protein, 3- phosphoglycerate dehydrogenase, serum albumin, ATP synthase, myosin, the enolase expression.

从腰椎松质骨蛋白质组表达分析可知,肌动蛋白、角蛋白、烯醇化酶、ATP合酶、肌球蛋白的表达增强可能与去卵巢后腰椎松质骨骨质疏松的发生相关;强骨宝对腰椎骨质疏松的干预效应可能与下调碳酸酐酶、肌动蛋白、αB-晶体蛋白、3-磷酸甘油醛脱氢酶、血清白蛋白、ATP合酶、肌球蛋白、烯醇化酶的表达有关。

Methods seventy-two 15-week-old sd rats were divided into six groups,included control group and five ovariectomized model groups.the model groups were fed with different-dose lycopene[10,15,20 mg/] for 12weeks.then related indexes including length bone mineral density,bone mineral,serum estradiol,sertum alp and uterus index were analyzed.results compared with model group,three different dose groups and nilestriol[1.05mg/] group showed increased bone mineral density,bone mineral,serum estradiol and uterus index,but decreased serum alp (p.05).conclusion as a female sex hormone-like component,lycopene may ameliorate the bone quality and inhibit osteoporosis in ovariectomized rat.

将72只15周龄sd大鼠随机分为6组,空白组做假手术,其余5组做卵巢切除术,术后用药12周后处死,测定右侧股骨骨密度、骨矿含量、血清雌二醇、碱性磷酸酶含量及子宫湿重。结果番茄红素高[20mg/]、中[15mg/]、低剂量组[10mg/]、尼尔雌醇组[1.05mg/kg·bw]能对抗骨密度、骨矿含量、血清雌二醇的下降、血清碱性磷酸酶的升高,与模型组比较,p.05;番茄红素各剂量组、尼尔雌醇组能对抗子宫指数的减轻,与模型组比较,p.05。结论番茄红素可以改善骨质量,抑制大鼠去卵巢骨质疏松的发生。具有类似雌激素的效应。

Objective To explore the effects of Kang Gusong Pellet on bone quality and biomechanics performances of the lumbovertebral bone in ovariectomized rats.

目的 探讨抗骨松颗粒对去卵巢大鼠骨质疏松模型骨质含量和骨生物力学性能的影响。

The edgewise technique was used to align and level the upper and lower arches gradually and upright the lower incisors. Then, modified LeFort Ⅰ osteotomy was operated with 4 cases pterygomaxillary disjunction and the other 2 cases not. The maxillas were not moved during the operation and sutured mucoperiosteum at the same place. After 3-5 days latency period, elastic distraction about 700g/side and increasing to 1400g/side gradually, was started with two cases which the maxillas were not cut off totally.

术后经过3—5天的延迟期即开始牵引,2例行不完全截开术者,采用弹性牵引,力量从700g/侧逐渐增加至1500g/侧;4例行完全骨截开术者,首先采用刚性牵引和口外牵引钩,每天缩短牵引钢丝1mm,2例直接达到矫治要求,其余2例达到浅覆盖后,截去口外牵引钩,换弹性牵引和口内牵引钩继续前牵上颌骨。

Results:(1) The XLGBC can obviously increase the estradiol of serum in the castrational animal;(2) The XLGBC can obviously increase the spermathecal gland coefficient and the ovary coefficient in the Yangxu animal;(3) The XLGBC can obviously increase the spermathecal gland coefficient and the ovary coefficient in the Yinxu animal.

结果 (1)对去势动物模型,仙灵骨葆胶囊明显提高血清雌二醇水平;(2)对阳虚动物模型,仙灵骨葆胶囊明显提高阳虚小鼠精囊腺和卵巢的系数;(3)对阴虚动物模型,仙灵骨葆胶囊明显提高阴虚小鼠精囊腺和卵巢的系数。

Methods:(1) The two-sided ovary of female animal and the two-sided testicle of male animal were excised. After the XLGBC to treat 28 days, the estradiol and the teststerone of serum were checked;(2) With intramuscular injection the Hydrocortisone,the Yangxu animals was made. After the XLGBC to treat 10 days, the testicle coefficient,the spermathecal gland coefficient and the ovary coefficient were observed;(3) With ig the Thyroid tablets and the Reserpine, the Yinxu animal was made. After the XLGBC to treat 10 days, the testicle coefficient,the spermathecal gland coefficient and the ovary coefficient were observed.

(1)雌性动物摘除双侧卵巢,雄性动物摘除双侧睾丸,复制成去势动物模型后予仙灵骨葆胶囊治疗28天,检测血清雌二醇和血清睾酮;(2)肌注氢化可的松,复制成阳虚动物模型后予仙灵骨葆胶囊治疗10天,观测并计算动物的睾丸系数、精囊腺系数和卵巢系数;(3)灌胃甲状腺片和利血平,复制成阴虚动物模型后予仙灵骨葆胶囊治疗10天,观测并计算动物的睾丸系数、精囊腺系数和卵巢系数。

Whereas osteoporosis is unassociated with bone pain, osteomalacia has been associated with isolated or generalized bone pain.39,40 The cause is thought to be hydration of the demineralized gelatin matrix beneath the periosteum; the hydrated matrix pushes outward on the periosteum, causing throbbing, aching pain.7 Osteomalacia can often be diagnosed by using moderate force to press the thumb on the sternum or anterior tibia, which can elicit bone pain.7,40 One study showed that 93% of persons 10 to 65 years of age who were admitted to a hospital emergency department with muscle aches and bone pain and who had a wide ariety of diagnoses, including fibromyalgia, chronic fatigue syndrome, and depression, were deficient in itamin D.41

然而,骨质疏松症与骨痛无关联,而软骨病则与局部性或全身性骨痛有关。其原因被认为是骨膜下已去矿质化的胶原基质上发生的水合反应,水合的胶原基质在骨膜上向外扩张,引起阵痛。软骨病可通过以拇指适度挤压胸骨和胫骨前方以引起骨痛感。一项研究显示10岁到65岁中有93%的人向医院急诊室承认有肌肉疼痛和骨痛症状,他们还有一些其他症状包括纤维肌痛、慢性疲劳综合征、抑郁等,该研究显示他们都缺乏维生素D。本人已认领该文第9、10两部分,48小时后若未提交译文,请其他战友自由认领。本人已认领该文11、12部分编译,48小时后若未提交译文,请其他战友自由认领。本人认领第十三部分,48小时内交稿请战友纠错!

Methods: thirty-four Japanese white rabbits are used as experimental animals. Under no destroying the posterior ligaments, expose the fifth and sixth transverse processes and intertransverse membrane. The transverse processes are decorticated. On each side bone graft bed the following four kinds of grafts are put, they are: autologous iliac crest、allograft+rhBMP-2、xeograft+rhBMP-2、and xeograft+HA.

34只成年日本大耳白兔,在不破坏脊柱后侧稳定结构的前提下,显露双侧的L5、L6横突,用磨钻去皮质,然后每侧分别随机地植入2.5ml左右的下列四种植入物:自体髂骨、同种冷冻干燥异体骨+rhBMP-2、异种冷冻干燥骨+rhBMP-2、异种冷冻干燥骨+HA。

The residual calcium and phosphorus contents of bone particles were measured after degreased and demineral ized.

取猪四肢长管状骨皮质骨经理化处理后,制备脱脂脱钙和脱脂脱钙去细胞两种粒径为250 ~ 810 μm 的DBM 颗粒和浸提液,骨颗粒脱脂脱钙后测钙、磷含量。

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