去血浆法

To develop a HPLC method for determination of deferiprone concentration in dialysate and to study the plasma protein binding rate of DFP.

目的建立测定透析液中去铁酮浓度的高效液相色谱法，观察DFP的大鼠血浆蛋白结合率。

A sample-pretreated method of ether-extraction-precolumn-derivation was established, and the chromatographic conditions, which was used for determinating the derivant of extracted UDCA, were decided to be Hypersil C_(18) column(250×4.6mm, 10μm), acetonitrile-methol-0.005mol·L~(-1) phosphoric buffer-triethylamine(50:27:42:0.25, adjusted to pH3.0±0.1 by phosphoric acid) as mobile phase with detective UV wavelength at 247nm and column temperature at 35℃.

建立了乙醚提取-柱前衍生化血浆样品预处理方法，采用Hypersil C_(18)柱(250×4.6mm，10μm)，乙腈-甲醇-0.005mol·L~(-1)NaH_2PO_4缓冲液-三乙胺(50：27：42：0.25，磷酸调节pH3.0±0.1)为流动相的HPLC法，以溴苯乙酮为衍生化试剂，在247nm处测定家兔血浆中熊去氧胆酸衍生化产物。

HR、MAP、ECG、SPO_2、PETCO_2 and Ppeak were monitored and the venous blood samples were obtained to measure serum concentrations of epinephrine, NE, IL-6 and IL-10 by ELISA at different perioperation time: 5 min before anesthesia (T_0), before the CO_2 was insufflated into peritoneal cavity (T_1)and 5 minutes after(T_2),20 minutes after(T_3), the end of peritoneal insufflation (T4)and 30 minutes after the end of peritoneal insufflation(T_5). Blood samples from artery were obtained atT_1、T_2、T_3、T_4、T_5 for blood gas analysis.

于麻醉前5min(T_0)、气腹前5min(T_1)、气腹开始后5min(T_2)、气腹开始后20min(T_3)、气腹结束时(T_4)及气腹结束后30min(T_5)，抽静脉血标本，分离血浆，用ELISA法测肾上腺素、去甲肾上腺素、细胞因子IL-6、IL-10浓度，并于T_1、T_2、T_3、T_4、T_5各时点抽动脉血行血气分析。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。