去神经

However, we observed distinct abnormalities within presynaptic terminals of spinal MNs at the neuromuscular junction, as well as profound denervation.

无论如何，我们观察到神经肌肉接头处的脊髓运动神经元突触前末梢的明显的异常，还有显著的去神经支配。

Most of thesemodels rely on a partial denervation of the limb of the animalby ligating a selected nerve.

绝大多数的这些模型依赖于结扎所选择的神经使物肢体部分去神经。

An enriched PCR fragments with less complexity were obtained and mainly expressed in the denervated hippoampus After cloning into the plasmid vector, 46 clones were initially screened and subjected to autosequencing By comparison with the EMBO gene databases, 21 gene fragments of different types were obtained with 11 similar to genes of known function and 10 similar to ones of unknown function It is inferable that the altered gene expressions of hippocampus following the entorhinally denervation may underlie the specfic reinnervation and the formation of synapses by transplanted entorhinal neurons with host neuropil within the denervated target areas.

初步挑选出的46个转化后的阳性克隆，经点杂交筛选和基因序列分析，与Gene Database同源性比较后，得到21种基因片段，其中功能已知的有11种，功能未知的有10种。结果表明海马在去内嗅皮层后某些基因表达的改变，这可能是移植胚胎内嗅皮层神经细胞轴突特异支配去神经靶区和重建突触联系有关。

Bacterial pyrogen was injected into denervated rabbbitear and novocain blocked rabbit knee joint capsule to see whether pyrogen induces fever through nervous reflex mechanism via local receptors or not.

为观察细菌致热原是否通过局部感受器——神经反射机制引起发热而进行下列实验：把细菌致热原注入去神经兔耳和完整神经兔耳皮下；把细菌致热原注入事先用奴弗卡因封闭的兔膝关节囊和事先注射生理盐液的兔膝关节囊；分别比较两组动物的发热效应。

8 Fold in the hippocampus 10 days following perforant path injury, 32 were up-regulated and 152 down-regulated. Roughly the function categories of the target genes are: immunity reaction, environmental response, cell structure, membrane transport and protein turnover.

CustomcDNA array筛选的8000个cDNA克隆中有47个在去神经30天海马显示差异表达且表达变化在3倍以上，其中25个表达上调，22个表达下调；Northernblot实验表明cDNA array的假阳性率是较高的。cDNA microarray在大鼠中枢神经系统表达的6234种基因和EST序列中，筛选到了152个基因和EST序列在去神经10天海马差异表达，表达变化在1.5-2.8倍之间，其中表达上调的32个，下调的120个。

Of 8000 cDNA elements, custom cDNA array identified 47 as differentially expressed genes/ESTs with above 3-fold changes in the hippocampus 35 days after lesion, in which 25 were up-regulated and 22 down-regulated. Northern blot hybridization indicates that false ratio is high with the cDNA array experiment. Of 6234 rat CNS genes/ESTs, cDNA microarray identified 152 as differentially expressed cDNA clones with changes between 1.5-2.8 fold in the hippocampus 10 days following perforant path injury, 32 were up-regulated and 152 down-regulated.

Custom cDNA array 筛选的8000个cDNA 克隆中有47个在去神经30天海马显示差异表达且表达变化在3倍以上，其中25个表达上调，22个表达下调； Northern blot 实验表明cDNA array 的假阳性率是较高的。cDNA microarray在大鼠中枢神经系统表达的6234种基因和EST序列中，筛选到了152个基因和EST 序列在去神经10天海马差异表达，表达变化在1.5-2.8倍之间，其中表达上调的32个，下调的120个。

To elucidate the molecular mechanisms underlying the neural plasticity after CNS injury, the gene expression profile in the rat hippocampus following perforant path transections was explored by several methods including custom differential screening, custom cDNA array and cDNA microarray. Of 2300 cDNA clones from low-abundance rat brain cDNA bank, 6 were identified as differentially expressed genes/ESTs in the hippocampus 35 days after lesion, in which none was confirmed by Northern blot. Of 8000 cDNA elements, custom cDNA array identified 47 as differentially expressed genes/ESTs with above 3-fold changes in the hippocampus 35 days after lesion, in which 25 were up-regulated and 22 down-regulated.

中文题名去传入海马的差异表达基因筛选和Cystatin C表达副题名外文题名 Screening of the differentially expressed genes and cystatin C expression in the deafferented hippocampus 论文作者应国新导师周长福研究员学科专业神经生物学研究领域\研究方向学位级别博士学位授予单位中国科学院上海生命科学研究院学位授予日期2002 论文页码总数121页关键词海马星型胶质细胞 Cystatin C 基因表达去神经海马馆藏号BSLW /2003 /Q42 /27 为了阐明中枢神经系统损伤后神经可塑性的分子机制，本实验探索了多种方法，包括传统差异筛库、传统cDNA array和cDNA microarray，对穿通纤维切断后大鼠海马的基因表达图谱进行了研究。

The specimen were examined with light and electronmicroscope.Results The metabolism of the denervated muscle was lower than the normal.

结果 去神经后肌肉的代谢降低；移植到有正常神经支配的肌肉内12周后有神经长入。

Objective:To investigate the effects of denervated skeletal muscle extract on proliferation of Schwann cells in vitro and elucidate the mechanism of proliferation of SCs after peripheral nerve injury.

目的 ：观察去神经支配骨骼肌粗提液在体外培养条件下对雪旺细胞(Schwanncells，SCs)增殖的作用，了解外周神经损伤后SCs大量增殖的机理。

The results indicated that:(1) 4 d or 6 d after denervation, increase of endocyto--sis and proliferation of satellite cells in denervated muscle were induced.(2) Actino--mycin D inhibited activation of satellite cells and endocytosis in normal muscle.

结果表明：(1)去神经4d或6d的肌肉可引起胞纳的增加和卫星细胞的增殖；(2)放线菌素D抑制正常肌肉的卫星细胞激活和胞纳作用；(3)在去神经的肌肉中，放线菌素D抑制了卫星细胞增殖的同时还抑制了胞纳的增加，但不能阻止去神经肌肉的萎缩。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。