去垢剂

CHAPS efficiently solubilized the P450 of microsomes in midgut and fatbody, while lubrol PX , emulgen 911 and sodium cholate were less efficient.

对CHAPS、Lubrol PX、Emulgen 911和胆酸钠四种去垢剂的比较研究发现：CHAPS对中肠和脂肪体微粒体P450的增溶效率最高，Lubrol PX、Emulgen 911和胆酸钠较差。

Four detergents above mentioned didn't denature P450 of microsomes in midgut and fatbody at concentration of 0.5%.

在0.5％浓度下，四种去垢剂均未使中肠和脂肪体微粒体P450变性。

In this paper the effect of gangliosides on the erythrocte membrane injured by detergent Triton X- 100 is studied.

观察了神经节苷脂对去垢剂Triton—X—100损伤大鼠红细胞膜的影响。

Tissue engineering cornea stromata were constructed in vitro.

用去垢剂联合酶的方法脱细胞处理猪角膜基质，在去细胞猪角膜基质材料上接种体外培养的兔角膜基质细胞，并将兔角膜基质细胞进行PKH26荧光标记，在体外构建组织工程角膜基质。

METHODS: Porcine cornea stromata were processed by serial digestion of trypsin, DNase and RNase, and then were implanted into corneal stroma layer of New Zealand rabbits.

将用去垢剂联合酶的方法脱细胞处理的猪角膜基质材料植入兔角膜基质囊袋中，进行裂隙灯观察并照相。

Using the cationic detergent benzyldimethyl -alkylammonium chloride instead of cytochrome C in spreading procedure, we have observed the binding site for EcoR Ⅰ on linear pBR322 DNA molecule in the presence of gluteraldehyde. The diameter of the bound EcoR Ⅰ molecules are 15nm which may be the tetrameric EcoR Ⅰ particles.

用低分子量阳离子去垢剂苯二甲基苄基氯化胺取代经典展层技术中的细胞色素C，汲取前人经验，建立了无蛋白展层技术，适用于蛋白质--DNA相互作用的电镜研究。

Treating the trimeric LHCⅡ with phospholipase A2(PLA2) could induce the dissociation of the complexes.

用去垢剂增溶及高速离心的方法，从菠菜类囊体膜中提取并纯化了光系统Ⅱ的捕光天线LHCⅡ异质三聚体。

In this paper, purification of growth hormone receptor from ovine liver and identification of its biochemical character have been done.Firstly, by homogenizing, triturating, high-speed centrifuging, hypervelocity centrifuging and sucrose density grade centrifuging cell membrane of ovine liver was separated and its electron microscope slice wasprotein was disL~olved with TritonX-100,amL-thebinding activity between cell membrane or membrane protein and ~I-hGH using RRAwas measured, perfect saturated curve wasgained. Thirdly, specific binding of GILR protein was measured ,by SDSPAGE, its molecular weight is about 1 19KD.

本研究采取新鲜羯羊肝脏，匀浆、研磨，经高速离心，超速离心和蔗糖密度梯度离心，分离肝脏质膜，得到肝脏质膜的电镜切片：其次，用去垢剂TritonX-100增溶膜蛋白，利用放射受体分析法，测定细胞膜及膜蛋白与~(125)I-hGH的结合活性，均得到理想的饱和曲线；再次，将两点结合试验法与凝胶层析法结合检测到特异结合物，然后，经SDS-PAGE电泳分析，纯化后的样品呈现一条带，与标准分子量比较，得出分子量为119KD。

The ciliates were always ideal materials in the studies of the structure and function of cytoskeleton and its cell control and adjustment, for they have many kinds of cytoskeletons with complexity and they could be easily cultured and handled.

由于原生动物纤毛虫具有多种复杂的细胞骨架结构，加之其材料培养和处理的优点，一直被作为研究细胞骨架结构与功能及其细胞调控的理想材料。20世纪80年代初，许多学者应用蛋白银染色方法和超微结构方法，显示了纤毛虫细胞皮层中以纤毛基体作为主成分的骨架结构，探讨了纤毛虫皮层纤毛器的形态、形态发生及其结构形成机理，在国内外原生动物细胞学领域成为主流。20世纪90年代起，先后应用非离子去垢剂处理和扫描电镜相结合的方法、稳定保存微管结构的透射电镜术，微管蛋白的抗体标记及其免疫荧光技术及相关的生化与分子生物学等方法显示纤毛虫的微管胞器及其微管蛋白组分，探索微管胞器的形态、功能及其微管装配机理，积累了较多的资料，取得了重要的进展。

The results are as follows: the DDM detergent dissolves the Cyt b6f better thanβ-OG detergent does; DDM can keep the Cyt b6f in native state, under which the hemes and Chl a experience specific environment, while theβ-OG make the complex in denatured state in some sort, under which the catalyzed electron transfer activity of the complex

结果表明，DDM去垢剂能使Cyt b6f处于较好的分散体系中，其中血红素和Chl a分子处于特定的蛋白环境中，不会导致Cyt b6f变性；而β-OG去垢剂会使Cyt b6f产生聚合现象，其中的血红素和Chl a与蛋白环境的相互作用减弱，和DDM相比其电子传递活性显著降低，Chl a单线激发态寿命延长，Chl a更容易被光

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。