去...

The results showed that 1 there were no significant differences in the rates of cytoplast protrusion and enucleation between oocytes that were incubated in colchicine (0.4 μg/mL) for 0.5 h and oocytes that were incubated in colchicine (0.4 μg/mL) for 1 h, and the rate of cytoplast protusion can be 85.4% while the rate of cytoplast enucleation is 100%. 2 There was no significant difference in oocyte enucleation between oocytes treated with medium containing 0.2 μg/mL colchicine for 0.5 h and oocytes treated with medium containing 0.4 μg/mL colchicine for 0.5 h. 3 A maturation time of 18–23 h did not affect the rates of cytoplast protusion and enucleation by chemically assisted enucleation, whereas the rate of enucleation of oocytes by blind enucleation was found to decrease with a prolonged incubation time. 4 The development rates of reconstructed embryos could not be influenced by these two enucleation methods, increased from oocytes matured for 21–23 h.

结果表明： 1 卵母细胞在0.4 mg/mL的秋水仙素溶液中分别孵育0.5 h和1 h，胞质突起率和去核率没有显著的差异，突起率可高达85.4%，去核率达到100%； 2 0.2 mg/mL或0.4 mg/mL秋水仙素溶液将卵母细胞处理0.5 h，对去核效果没有显著影响； 3 对于体外成熟18~23 h的卵母细胞，随着成熟时间的延长，盲吸法的去核率降低，但没有影响秋水仙素诱导胞质突起的比率和去核率； 4 两种去核方法对重构胚的发育没有产生显著影响，但成熟21~23 h卵母细胞重构胚囊胚的发育率显著高于成熟18~20 h卵母细胞重构胚囊胚的发育率。

There was me and my monkey And with his dungarees and roller blades Smoking filter tips Reclining in the passenger seat of my super charged jet black Chevrolet He had the soft top down He liked the wind in his face He said son you ever been to Vegas I said no He said that's where we're gonna go You need a change of pace And when we hit the strip with all the wedding chapels and the neon signs He said left my wallet in El Segondo and proceeded to take two grand of mine We make tracks to the Mandalay Bay Hotel Asked the bell boy if he take me and my monkey as well He looked in the passenger seat of my car And with a smile he said If your monkey's got that kind of money sir Then we've got a monkey bed Me and my monkey With a dream and a gun I'm hoping my monkey don't point that gun at anyone Me and my monkey Like butch and the sundance kid Trying to understand why he did what he did Why he did what he did We got the elevator, I hit the 33rd floor We had a room up top with the paronama views like nothing you'd ever seen before He went to sleep in the bidest And when he awoke He ran his little monkey fingers through yellow pages Called up some escort services and ordered some okey doke Forty minutes later there came a knock at the door In walked this big bad ass baboom into my bedroom with three monkey whores Hi, my name is Sunshine, these are my girls Lace my palm with silver baby and oh yeah They'll rock your world So I watched pay per view and polished my shoes and my gun Was diggin' on Kurt Cobain singing about lithium There came a knock at the door and in walked Sunshine What's up you'd better get your ass in here boy Your monkey's having too much of a good time Got tickets to see Sheena Easton The monkey was high Said it was a burning ambition to see her before he died We left before encores he couldn't sit still Sheena was a blast baby but my monkey was ill We went to play blackjack kept hitting twenty three Couldn't help but notice this Mexican just staring at me Or was it my monkey I couldn't be sure It's not like you'd never seen a monkey in rollerblades and dungarees before Now don't test my patience cause we're not about to run That's a bad ass monkey boy and he's packing a gun My name is Rodrigue he says with death in his eye I've been chasing you for long time amigos And now your monkey's gonna die Me and my monkey Drove in search of the sun Now me and my monkey We don't wanna kill no Mexican But we've got Ten itchy fingers one thing to declare When the monkey is high You do not stare you do not stare You do not stare Looks like we've got ourselves a Mexican stand off here boy And I ain't about to run Put your gun down boy How'd I get mixed up with this fuckin' monkey anyhow

有我和我的猴子并且与他的dungarees 和路辗刀片抽烟过滤器要诀斜倚在我的超级被充电的喷气机黑色薛佛列汽车乘客座位他下来有软的上面他喜欢风在他的面孔他说您曾经是到维加斯I 说没有的儿子他说we're 去去的that's 您需要步幅的变动并且当我们击中了小条以所有婚礼教堂和霓虹灯广告他左说我的钱包在El Segondo 和进行采取二盛大我的我们做轨道对曼德勒海湾旅馆问响铃男孩如果他采取我并且我的猴子他看了在我的汽车乘客座位并且以微笑他说如果您的monkey's 得到了那金钱先生然后we've 得到了猴子床我和我的猴子与梦想和枪 I'm 希望我的猴子don't 把那杆枪指向任何人我和我的猴子象butch 和sundance 孩子设法了解为什么他做了什么他为什么他做了什么他我们得到了电梯，我击中了第33 楼我们有一间屋子上面以paronama 看法象没什么you'd 以前曾经被看见他去睡觉在bidest 并且当他醒了他跑了他小的猴子手指通过黄页召集了一些伴游服务和定购了一些okey doke 那里四十分钟以后来了敲在门在走了这大坏驴子baboom 入我的卧室与三个猴子妓女喂，我的名字是阳光，这些是我的女孩系带我的棕榈与银色婴孩和oh 呀 They'll 岩石您的世界如此我观看了薪水每看法和擦亮了我的鞋子和我的枪是diggin'在Kurt Cobain 唱歌关于锂那里来了敲在门和在走的阳光里 What's you'd 更好得到您的驴子在这里男孩您的monkey's 有太多好时光被得到的票看见Sheena Easton 猴子是高的前述这是灼烧的志向看见她在他死了之前我们离开在他couldn't 坐直的encores 之前 Sheena 是疾风婴孩但我的猴子是不适我们去演奏大酒杯继续击中二十三 Couldn't 帮助但注意这墨西哥人凝望我或是它I couldn't 是肯定的我的猴子 It's 不是喜欢you'd 以前从未被看见一只猴子在rollerblades 和dungarees 现在don't 测试我的耐心起因we're 不跑 That's 坏驴子猴子男孩和he's 包装枪我的名字是他说以死亡在他的眼睛的Rodrigue I've 追逐您为久时间朋友并且现在您的monkey's 去死我和我的猴子驾驶了寻找太阳现在我和我的猴子我们don't 不想要杀害墨西哥人但we've 得到了十个痒的手指一件事宣称当猴子是高的您不凝望您不凝望您不凝望看起来象we've 这里得到了墨西哥立场男孩并且I ain't 跑把您的枪男孩放下 How'd I 得到与这fuckin'混合；猴子无论如何或有我和我的猴与他dungarees和滚刀片吸烟过滤嘴斜倚在客运座位我国超级控喷射黑色雪佛兰他有软顶下来，他最喜欢的风在他的面对他的儿子说：你到过赌城我说没有，他说哪里，我们gonna你走需要改变步伐，而当我们打带所有的教堂婚礼和霓虹灯他说离开我的钱包在El segondo进而采取两项盛大的矿井，使我们路轨次电子曼德勒湾酒店询问钟男孩，如果他把我和我的猴子以及他的表情，在次电子乘客座位上我的车，面带微笑，他说，如果你的猴得到这笔钱思住宅，然后我们拿到猴床上，我和我的猴同一个梦想和一支枪，我希望我的莫 nkey不点炮人，在我和我的猴像布奇和森丹斯孩子设法了解后WH y他做他为何他做了什么，我们得到了电梯，我打到33楼，我们有一个房间顶与paronama看法没有像您见过会捶他睡在bidest而当他醒来，他跑他的小猴子手指透过黄坝天麻打电话询问过保姆服务，并订购了一些东西doke四十分钟左右，有一个前来敲门做或者走这个大坏驴子baboom到我的卧室有三名妓女猴喜，我的名字是阳光，硒是我的女孩花边我的手心与银宝宝，哦噢人家，你的摇滚世界，让我眼睁睁看着付每意见，并擦拭鞋子，我的枪是diggin '库尔特罗克塞特歌唱锂出现了一针玉在门口，并走在阳光下是怎么回事，你最好明白你的驴子在这里你男童和尚安永的有太大的一个好时机得到入场券见sheena伊斯顿猴高表示，这是燃烧的雄心，看到她生前我们离开之前，以encores他不能坐视sheena仍然是一个基本法 AST的婴儿，但我的猴子生病，我们去发挥酒杯一直打到23不禁通知书策墨这只是瞪着我，抑或是我的猴子，我也不能肯定它不像您在Neve 住宅看到一只猴子在晒太阳和dungarees之前，现在不考验我的耐心，我们的事业不是对办好次下午的一个坏驴子猴男孩和他的包装带枪我的名字是罗德里格说，他的死亡与他眼睛，我一直追你很久地球，现在你的猴死gonna我和我的博士猴脏器寻找太阳，现在我和我的猴子，我们不想杀任何墨西哥，但我们拿到碲氮手指发痒一回事申报时，猴子是高你不盯着你不盯哟美国不瞪眼样子，我们拿到了自己的立场，墨西哥产的男孩在这里，我并不是对办好把你的枪下男孩，我为什么会有这种攻防得到混为一谈这fuckin '猴不管怎样

Everyday besides have a meal to sleep even if get online, resemble of course I can give the door on the weekend, like to go out very much before me journey but time is closer now, hardly what sky goes out, bar also did not go, KTV gets collect to just fall to poll, the most place that goes possibly is cinema and odeum, also often a person goes, feel to be done from personal pretty laugh, nevertheless a desire wants to issue Sichuan namely now, because oneself aided financially a few children to read in a few years, this year Sichuan elementary school of a child there graduated quickly, I want to see him namely, but according to present condition for, do not know to be able to be met really give time to go for nothing.

天天除了吃饭睡觉就是上网，当然像周末我会出一下门，我以前很喜欢出门旅行但是现在时间比较紧，几乎没什么空出去，酒吧也不去了，KTV得凑到人头才去下，可能去的最多的地方就是电影院和音乐厅，也经常是一个人去的，觉得自己蛮搞笑的，不过现在有个愿望就是想去下四川，因为几年里自己资助了几个孩子读书，今年四川那边一个孩子小学就快毕业了，我就是想去看下他，可按照现在的状态来说，不知道会不会真会空出时间来过去了。

Go back to Mississippi, go back to Alabama, go back to South Carolina, go back to Georgia, go back to Louisiana, goback to the slums and ghettos of our northern cities, knowing that somehow this situation can and will be changed.

回到密西西比去吧；回到亚拉巴马去吧；回到南卡罗来纳去吧；回到佐治亚去吧；回到路易斯安那去吧；回到我们北方城市中的贫民窟和黑人居住区去吧。要知道，这种情况能够而且将会改变。

Go back to Mississippi, go back to Alabama, go back to Georgia, goback to Louisiana, go back to the slums and ghettos of our northerncities, knowing that somehow this situation can and will bechanged.

回到密西西比去吧；回到亚拉巴马去吧；回到南卡罗来纳去吧；回到佐治亚去吧；回到路易斯安那去吧；回到我们北方城市中的贫民窟和黑人居住区去吧。要知道，这种情况能够而且将会改变。

To right the unrightable wrong To be better far than you are To try when your arms are too weary To reach the unreachable star This is my quest, to follow that star, No matter how hopeless, no matter how far To be willing to give when there's no more to give To be willing to die so that honor and justice may live And I know if I'll only be true to this glorious quest That my heart will lie peaceful and calm when I'm laid to my rest And the world will be better for this That one man scorned and covered with scars Still strove with his last ounce of courage To reach the unreachable star.

去做那不可能的梦想去和那打不败的敌人战斗承担那无法承受的哀愁奔向那勇者们都不敢前去的地方去改正那无法修正的错误从远处献上纯洁的爱在你的双臂都已疲累的时候继续努力伸手去探取那遥不可及的星星这就是我的理想去追寻那颗星星不论希望多麽渺茫不论路途有多遥远没有疑惑永不休止的为正义而战斗为了天赋的使命即使被打入地狱也心甘情愿而我知道只要我忠於这璀璨的梦想当我被安葬的时候我的心将会宁静祥和而世界也将变得更加美好因为有个备受责难满身创伤的人仍然在拼着他最后一丝的勇气去探取那遥不可及的星星

Where our dogmatists err on this question is that, on the one hand, they do not understand that we have to study the particularity of contradiction and know the particular essence of individual things before we can adequately know the universality of contradiction and the common essence of things, and that, on the other hand, they do not understand that after knowing the common essence of things, we must go further and study the concrete things that have not yet been thoroughly studied or have only just emerged.

例如，无产阶级和资产阶级的矛盾，用社会主义革命的方法去解决；人民大众和封建制度的矛盾，用民主革命的方法去解决；殖民地和帝国主义的矛盾，用民族革命战争的方法去解决；在社会主义社会中工人阶级和农民阶级的矛盾，用农业集体化和农业机械化的方法去解决；共产党内的矛盾，用批评和自我批评的方法去解决；社会和自然的矛盾，用发展生产力的方法去解决。

" They went to the camp in black, but they came back to the town in white; they went down the camp in ropes, they came back in chains of gold; they went down to the camp with their feet in fetters, but came back with their steps enlarged under them; they went also to the camp looking for death, but they came back from thence with assurance of life; they went down to the camp with heavy hearts, but came back with pipe and tabor playing before them"

&他们去营地时是夜里，但是他们回到镇上的时候是白天；他们去营地时带着绳索，他们回来时带着金链子；他们去营地时脚上带着脚镣，但是回来时却大步流星；他们去营地时以为会死去，但是他们回来时却获得了生命的保证；他们去营地时，心情沉重，但是回来时去看到了人们敲锣打鼓。&

Go back to Mississippi, go back to Alabama, go back to South Carolina go back to Georgia, go back to Louisiana, go back to the slums and ghettos of our modern cities, knowing that somehow this situation can and will be changed.

让我们回到密西西比去，回到阿拉巴马去，回到南卡罗来纳去，回到乔治亚去，回到路易斯安那去，回到我们北方城市中的贫民区和少数民族居住区去，要心中有数，这种状况是能够也必将改变的。

Go back to Mississippi, go back to Alabama, go back to South Carolina go back to Georgia, go back to Louisiana, go back to the slums and ghettos of our modern cities, knowing that somehow this situation can and will be changed.

回到密西西比去吧；回到亚拉巴马去吧；回到南卡罗来纳去吧；回到佐治亚去吧；回到路易斯安那去吧；回到我们北方城市中的贫民窟和黑人居住区去吧。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。