原质

A simple, rapid and accurate method for the determination of hydroxyproline in collagenous proteins by high performance liquid chromatography-electrospray mass spectrometry (HPLC-ESI/MS) was developed.

建立了一种简单、快速地测定胶原蛋白中羟脯氨酸的高效液相色谱-电喷雾质谱(HPLC-ESI/MS)方法。

So far, many reports have indicated that extracellular matrix accumulation in connective tissue, particularly the collagenous component, played the important roles in gingival overgrowth.

目前为止，大部分的研究结果指出在结缔组织细胞外间质特别是胶原蛋白的堆积在牙龈增生的过程中扮演重要的角色。

Objective: To contructre combinant plasmid pET28a/hES and coexpress human Endostatin and predhPK-5 in E. coli. Methods: mRNA from human liver tissue was exracted, and the Endostatin gene was amplified by RT-PCR.

目的构建人内皮抑素原核表达质粒pET28a/hES，并与简化人纤溶酶原饼环区(predigested human PlasminogenKringle5，predhPK-5)在大肠杆菌中实现共表达。

Objective To study the changes of behavior and expressions of glial fibrillary acidic protein in dorsal commissural nucleuse and thalamic intralaminar nuclei in formalin induced acute colitis in rats. Methods An animal model of acute colitis in visceralgia rats was used in this study.

目的 探讨因福尔马林引起的大鼠急性结肠炎刺激诱导的行为学变化及骶髓后连合核(dorsal commissural nucleuse， DCN)和丘脑板内核群(thalamic intralaminar nuclei， ILN)胶质原纤维酸性蛋白(glial fibrillary acidic protein， GFAP)的表达及其意义。

Objective: To study the changes in expressions of synaptophysin and glial fibrillary acidic protein after coriaria lactone induced status epilepticus, and the mechanism of SE.

目的：研究马桑内酯所致癫痫持续状态下海马结构内突触体素及胶质原纤维酸性蛋白表达的变化规律，探讨癫痫的发生机制。

Methods: Isolated rat costalchondral growth plate by microdissection, dispersed RGC (rat costochondral growth plate chondrocyte) were obtained by digestion (0.05% hyaluronidase and 0.2% collagenase type Ⅱ), inoculated and cultured in monolayer....

显微解剖分离出大鼠肋生长板，酶消化法(0.05%透明质酸酶和0.2%Ⅱ型胶原酶)获得分散的大鼠肋生长板软骨细胞(rat costochondral growth plate chondrocyte，RGC)，常规接种，单层培养。

Objective To investigate the clinical and pathological features and differential diagnosis of cryptogenic organizing pneumonia and conventional interstitial pneumonia.

目的 探讨隐原性机化性肺炎和普通型间质性肺炎的临床和病理特征以及鉴别诊断。

Ingredients: Arbutin 2%, Electrodialyzed Deep Sea Water, Mink Oil, Emu Oil, Borage Seed Oil, Calendula Officinalis Flower Oil, Lavandula Angustifolia Fiower Oil, Rosmarinus Officinalis Oil, Daucus Carota Sativa Seed Oil, Eucalyptus Globulus Leaf Oil, Pelargonium Graveolens Oil, Lecithin, Tocopherol, Hydrolyzed Collagen, Hydrolyzed Elastin, Ubiquinone Ferment, Squalene, Sodium Hyaluronate, Retinol, Glycine Soya Phytoplacenta Extract, Ceramide, Panthenol, Allantoin, Melaleuca Alternifolia Extract, Rosa Damascena Flower Extract, Propolis Extract, Centella asiatica L.

主要成分：含熊果苷2%，海洋深层水，水貂油， Emu Oil， Borage Seed Oil，金盏菊花油，薰衣草油，迷迭香精油，胡萝卜素油，蓝桉叶油，天竹葵香薰精油，卵磷脂，维生素E，水解胶原蛋白，辅酶Q10，角鲨烯，透明质酸钠，视黄醇，甘氨酸大豆提取物，神经酰胺，D-L泛醇，尿囊素，互叶白千层提取物，玫瑰果油，突厥蔷薇，蜂胶，积雪草，黄芩，甘草，金银花瓢虫，银杏叶，人参，洋甘菊，葛根等提取物

Ingredients: Electrodialyzed Deep Sea Water, Disodium Laureth sulfosuccinate, Coco-Betaine, Mink Oil, Emu Oil, Jojoba Oil, Calendula Officinalis Flower Oil, Lavandula Angustifolia Fiower Oil, Rosmarinus Officinalis Oil, Rose Hip Oil, Borage Seed Oil, Daucus Carota Sativa Seed Oil, Eucalyptus Globulus Leaf Oil, Pelargonium Graveolens Oil, Lecithin, Tocopherol, Hydrolyzed Collagen, Hydrolyzed Elastin, Ubiquinone Ferment, Squalene, Sodium Hyaluronate, Retinol, Glycine Soya Phytoplacenta Extract, Ceramide, Panthenol, Allantoin, Melaleuca Alternifolia Extract, Rosa Damascena Flower Extract, Propolis Extract, Morus alba L.

主要成分：含海洋深层水， Disodium Laureth sulfosuccinate，可可甜菜碱，水貂油， Emu Oil， Borage Seed Oil，金盏菊花油，薰衣草油，迷迭香精油，玫瑰果油，胡萝卜素油，蓝桉叶油，天竹葵香薰精油，卵磷脂，维生素E，水解胶原蛋白，弹性蛋白水解，辅酶Q10，角鲨烯，透明质酸钠，视黄醇，甘氨酸大豆提取物，神经酰胺，D-L泛醇，尿囊素，互叶白千层提取物，突厥蔷薇，蜂胶，薄荷，芝麻，何首乌，银杏叶，枸杞，荨麻腿子属，洋甘菊，葛根等提取物

Objective To study the relationship of endangium proliferation and the expression of metalloproteinases and tissue inhibitors of metalloproteinases after angioplasty of iliac artery in rabbits to investigate the probable mechanism of composite Danshen pill in prevention and treatment of restenosisMethods 30 white male rabbits of Japan （the average body weight was 25～30 kg）were randomly divided into 3 groups:normal control group,model group (intima destroyed by balloon and given hypercholesterol diet),CDP group (intima destroyed by balloon and given hypercholesterol diet plus drug CDP 150 mg/d),10 ones each groupResults The results of iliac artery angiogram and the analysis of pathology:there were lumens stenosis,thinner intima,smaller intima area,in CDP group compared with those in model group,and ratio of intima and tunica media thickness and area among each group (P＜001)Immunohistochemistry analysis:the MMP2,MMP9,TIMP1,TIMP2 in model group significantly increased than those in control group (P＜001)The CDP group had lower MMP2 and MMP9 expression,higher TIMP1 and TIMP2 expression,and increased MMP2/TIMP1,MMP9/TIMP2 than model group (P＜001)The thickening of vascular neointima and stenosis degree of vascular lumen were relevant to MMP2 and MMP9 (r=0896,P＜001)Conclusions MMP and TIMP play the very important role in the restenosis process of arteryCDP could inhibit the thickness of vascular neointima after balloon injury,the probable mechanism of which may be inhibiting collagen formation,smooth muscle immigration and decreasing hyperplasia of intima by interfering expression of MMPs and TIMPs

目的 研究兔髂动脉成形术后血管内膜增生和基质金属蛋白酶及其抑制物表达之间的关系，探讨复方丹参滴丸预防再狭窄的可能机制。方法健康成年二级雄性日本大耳白兔30只，平均体重25～30 kg。随机分为3组：正常对照组10只，模型组10只（球囊内膜剥脱加高胆固醇饮食），治疗组10只（球囊内膜剥脱加高胆固醇饮食以及复方丹参滴丸150 mg/d）。结果兔髂动脉造影、血管病理图像分析检测结果：①复方丹参滴丸组较模型组血管造影示管腔直径狭窄、内膜厚度减少、内膜面积减少、内膜厚度和中膜厚度比、面积比，各组之间有显著性差异(P＜001)。②免疫组化分析：模型组MMP2、MMP9、TIMP1、TIMP2表达均高于对照组（P＜001）；复方丹参滴丸组MMP2、MMP9的表达低于模型组，而TIMP1、TIMP2的表达高于模型组；TIMP1/MMP2、TIMP2/MMP9明显增加，差异有显著性（P＜001）。③内膜的增生以及管腔的狭窄程度与MMP2、MMP9有很好的相关性(r=0896，P＜001)。结论 MMP和TIMP在再狭窄形成中起重要作用；复方丹参滴丸能够明显抑制血管损伤后的内膜增生，可能的机制是通过影响金属蛋白酶MMP2、MMP9及其抑制物TIMP1、TIMP2的表达从而抑制胶原的生成、平滑肌的迁移、增生，而减少内膜的增生。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。