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Experimental liver fibrosis; stromelysin-1; proenzyme protein expression; Western blot

实验性肝纤维化;基质分解素-1;酶原表达; Western印迹法

Usually they exist as proenzyme, but after activation, they rapidly attack the blood brain barrier, degrade some matrix proteins of the basis membrane, break down the endothelial cell close connection protein, and promote the hydro-cephalus formation and phlogocyte infiltrating.

通常它们以酶原的形式存在,一旦活化,则迅速攻击血脑屏障,降解基底膜的一些基质蛋白,破坏内皮细胞的紧密连接蛋白,促进脑水肿的形成和炎细胞的浸润。

Main Efficiency: Contain deep sea natural plants and minerals, combined with vitamin B3 and provitamin B5,effectively remove dirt and oil from pores, regularize oil secretion, improve quality of oily skin.

主要功效:结合深海天然海洋植物及丰富矿物质,加入维他命B3、维他命原B5,有效排走毛孔内的堆积油脂及污垢,调节油脂的分泌,改善油性肤质。

Ingredients: green cucumber extracts, sodium hyaluronate, provitamin B5, sea lavender, fish cartilage essence, female thistle essence, whey protein.

成分:青瓜萃取精华、透明质酸钠、维他命原B5、海鞭草、鱼类软骨精华、雌蓟草精华、乳清蛋白。

RESULTS:①Retroviral vector EGFP-pLNCX2 transfected primary chondrocytes of rabbits,and high expression of transfected chondrocytes could be obtained through preliminary screening of G418.After being screened and expressing EGFP,chondrocytes kept normal morphology,with pseudopod adhering to the wall and matrix secreting vigorously.

结果:①重组逆转录病毒载体EGFP-pLNCX2转染原代兔软骨细胞,经G418初步筛选而获得的高表达格局,可见软骨细胞经过筛选和表达绿色荧光蛋白后,保持正常的形态学,细胞伸出伪足贴壁,基质分泌旺盛。

Rat tail collagen can induce EPC involved in immigration, proliferation and pullulation in angiogenesis. The three-diamension model of EPC can be used to angiogenesis research.

鼠尾胶原凝液可以诱导EPC参与血管新生的迁移、增殖、发芽等步骤;EPC三维基质模型可用于新生血管的研究。

Methods Mouse macrophages were stimulated with Mpe LAMPs to analyze the activitation of NF-κB, and the effect of pyrrolidine dithiocarbamate, an inhibitor of NF-κB, on NF-κB activation was also studied by indirect immunofluorescence and western blot.

用从穿透支原体中提取的脂质相关膜蛋白刺激小鼠巨噬细胞,经Western blot和间接免疫荧光等方法检测NF-κB是否被激活,以及NF-κB抑制剂PDTC对NF-κB活性的影响。

Results]the gross morphology of the tendons showed the experimental group had lesser adhesion and better reactiveness than that in the control group.the fibrin had degraded at 3 weeks. cell tracing showed that the labeled bone marrow derived mesenchymal stem cells remained viable and presented in the intratendinous region for at least 6 weeks, becoming more diffuse at later timepoints.at 3 weeks, collagen fibers appeared more organized and there were better morphometric nuclear parameters in the treatment group. at 6 and 12 weeks, there were no differences between the groups with regard to morphometric nuclear parameters.

结果]实验组相比对照组肌腱大体观察粘连差,肌腱活动性好。3周后纤维蛋白载体即降解,细胞示踪结果显示标记的骨髓间充质干细胞至少6周内仍可保持活性并存在于肌腱组织中,但之后逐渐扩散。3周时,实验组与对照组相比胶原纤维排列更为有序,且胞核形态结构更规则,但在6周及12周时,实验组与对照组胞核参数测定无统计学意义。3周时实验组比对照组具有更强的的生物力学特性,但之后则差别不明显。

Results there were unconsciousness, respiratory rhythm disturbance and hyporeflexia of pupil light reflex immediately after injury, and reactiveness decrease and activity retardation still existed even after resuming consciousness. at anatomical scene, subarachnoid hemorrhage or cerebroventricular haemorrhage were widespread. at an early stage, there were swelling, collapse, and axonal retraction ball formation at cortico-medulla junction, callosum, brainstem, and cerebellar white matter under microscope. but at the later stage, gitter cell proliferation and nest-like aggregation were major pathophysiological changes at focal brain tissue.

结果 伤后大鼠均即刻出现原发昏迷,其中2只于损伤后20 min内死亡,余持续时间1-30 min不等;伤后大鼠呼吸节律紊乱,瞳孔对光反射减弱或消失,醒后均有程度不等的反应性下降,肢体活动迟缓;肉眼可见广泛蛛网膜下腔出血或脑室出血;光镜下可见皮髓交界区、胼胝体区、脑干、小脑白质等部位的神经轴索有不同程度的肿胀、断裂、轴索球形成,后期有小胶质细胞增生,局部呈巢样聚集。

Mesenchymal stem cells in bone marrow, embryonic stem cells, and skeletal myoblasts are known sources of CM repletion; however, there are a number of critical problems for clinical application.

骨髓间充质干细胞,胚胎干细胞,骨骼肌肌原细胞均为已知的CM祖细胞;但是,在临床应用中存在许多关键问题。

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I didn't watch TV last night, because it .

昨晚我没有看电视,因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

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我比喻得过头了。