原质

In this study, the effect of nCDHA on chondrogenic differentiation of rat adipose-derived adult mesenchymal stem cells in alginate gel was evaluated. nCDHA/alginate composites containing different amounts of nCDHA were prepared and used to encapsulate rat ADAS. The chondrogenic differentiation of ADAS was enhanced after 7 days and the total collagen content increased after 14 days at 500 ppm of nCDHA.

中文摘要本研究先制备一系列nCDHA/alginate奈米复合材料以三维培养方式，探讨大鼠脂肪干细胞经过ㄧ周与二周培养后，软骨分化基因及软骨基质分泌(collagen，GAG)进行评估，结果发现500 ppm nCDHA可增加第7天软骨基因分化与第14天胶原蛋白增生。

The adventitia is the outermost layer of the artery and typically consists of a loose matrix of elastin, smooth muscle cells, fibroblasts, and collagen.

动脉外膜是动脉的最外层，典型上，包含了疏松的弹力蛋白基质、平滑肌细胞、纤维母细胞、以及胶原。

In addition, after stably expression of bFGF, the biological characteristics of BMSCs and bone marrow-derived osteoblasts were analyzed in the fields of morphology, proliferation, cell cycle, alkaline phosphatase, typeⅠ collagen and so on.

从形态、增殖特性和细胞周期、碱性磷酸酶、Ⅰ型胶原等方面分析稳定表达碱性成纤维细胞生长因子的骨髓间充质干细胞和骨髓源性成骨细胞生物学特性。

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METHODS: Adipose was obtained from regions surrounding epididymis, retroperitoneal space and nape after anesthesis by pentobarbital, and adipose-derived mesenchymal stem cells could be harvested through type Ⅰ collagenase digestion. ADSCs were incubated in vitro and purified by differential adhesion.

戊巴比妥耳缘静脉麻醉，依次无菌取出附睾附近脂肪垫、腹膜后脂肪、颈背部脂肪，I型胶原酶酶解法分离出脂肪源间充质干细胞（adipose-derived mesenchymal stem cells，ADSCs），进行体外传代后差速贴壁培养纯化。

Methods: Mesenchymal stem cells were isolated from human term placenta by digestion of collagenase Ⅱ and their unique growth characteristic of attaching to the wall of cell culture flask. The proliferation ability was detected by living cell number counting and propidium iodide staining. Their surface markers were detected by flow cytometry. The cells were induced to osteoblast with dexamethasone, antiscorbutic acid and β-sodium glycerophosphate. And they also were induced to adipocytes with dexamethasone and insulin. After induction, the cells were observed by Von Kossa staining and oil red O staining.

将人足月胎盘组织经胶原酶Ⅱ消化和贴壁培养法获取间充质干细胞，运用活细胞计数和碘化丙吮检测其增殖能力；采用流式细胞术检测其细胞表面标志的表达；用地塞米松、抗坏血酸及β-磷酸甘油诱导其向成骨细胞分化，并用Von Kossa染色进行鉴定；用地塞米松与胰岛素诱导其向脂肪细胞分化，并以油红O染色进行鉴定。

Objective To introduce a method for condition primary culture of astrocyte of optic nerve.

目的 介绍一种快速原代培养、分离、纯化视神经星形胶质细胞的方法。

Objective To study the relationship between GFAP and the malignant degree of astrocytoma.

目的 研究胶原纤维酸性蛋白与星形胶质细胞瘤恶性程度的关系。

This subject aimed at observing the behaviors of rats, working in coordination with Barbitone , and assay of neural matter to verify it has influence on improve the slumber.

本项目通过现代药理实验的方法，以行为学改变，与戊巴比妥类药物协同和神经递质的测定为指标，验证《三因方》卷九载温胆汤原方剂量是否具有促进睡眠的作用。

Acellular allogeneic dermal matrix is the dermal replacement of heterogenous human skin in which the cellular elements have been removed by special technique and basement membrane complex have been remained. It becomes a kind of 3D porous acellular dermal scaffold. It supports fibroblast infiltration, neovascularization and epithelialization in the absence of an inflammatory response.

异体脱细胞真皮基质（acellular allogeneic dermal matrix， ADM）来源于正常的异体皮肤组织，经过特殊的理化处理去除真皮中的细胞成分，保留原有的胶原三维结构和完整的基底膜，成为一种多孔的三维膜性支架，其空间网状结构有利于细胞的增殖、毛细血管增生，加速组织的愈合过程，移植后可以迅速被血管化和自体细胞重建。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。