原质

Matrix extracelluar phosphoglycoprotein, which is one of the matrix extracelluar non-collage-nous proteins, is expressed in bone, teeth and renal proximal convoluted tubules.

细胞外基质磷酸糖蛋白是一种细胞外基质的非胶原性磷酸化糖蛋白，主要表达于骨组织、牙组织和肾近球小管中，在骨形成矿化以及调节磷吸收方面发挥重要作用。

By using liposome-mediated gene transfection technology, plasmid pCMVSV40T/PUR containing SV40Tag was transfected in primary embryonic precartilaginous stem cells, while non-transfected cells served as negative controls.

利用脂质体介导基因转染技术将含有SV40Tag的质粒pCMVSV40T/PUR转染原代胚胎前软骨干细胞，以未转染细胞作阴性对照。

METHODS: Femur and tibia were obtained from Wistar rats for primary culture of BMSCs. MSCs at passage 15-20 were firstly incubated in preinduction medium, for 24 hours, and then incubated in induction medium containing α-MEM, 2% dimethyl sulfoxide and 200 μmol/L butylated hydroxyanisole.

取Wistar大鼠股骨和胫骨，进行骨髓间充质干细胞原代培养，传至第15~20代融合状态的间充质干细胞置于预诱导培养基中培养24 h后，换用诱导培养基。

Methods M. penetrans was cultured and its LAMPs were extracted. Mouse macrophages were stimulated with M. penetrans LAMPs to analyze the production of nitric oxide. Cell prolification was detected in M. penetrans LAMPs-stimulated mouse macrophages by MTT assay.

用SP-4培养基培养穿透支原体并提取其脂质相关膜蛋白，用提取的脂质相关膜蛋白刺激小鼠巨噬细胞，以ELISA法检测NO的产生，并用MTT法检测其对小鼠巨噬细胞增殖的影响。

Objectives:Through the detection of the expression level of interleukins-1β(IL-1β), matrix metalloproteinase 9(MMP-9) and nuclear factor kappa B in Synovium and the detection of the concentration of IL-6 and TNF-αin serum of collagen induced arthritis Wister rat, to investigate the relationship between IL-1β、MMP-9、NF-κB and arthritis index in the development of arthritis,and to understand the pathology of synoviocyte.This may provide an experimented proof for the diagnosis of clinical rheumatical arthritis. Methods: Thirty male Wistar rats were randomly divided into two groups,namely the model controlled group and the normal controlled group,three subgroups were randomly divided in model group and controlled group separately.

目的：通过对白细胞介素1β，基质金属蛋白酶-9，转录因子核因子κB在胶原性关节炎大鼠滑膜细胞表达水平的观察，同时监测同期血清细胞因子IL-6和TNF-α的浓度，旨在探讨白细胞介素1β，基质金属蛋白酶-9，转录因子核因子κB与RA发生发展及其与关节指数之间的相关性，了解炎性病变关节滑膜细胞的病理生理状态，进一步为类风湿关节炎的临床治疗提供实验依据。

Viral vector mainly contain adenovirus, retrovirus,adeno-associated virus and so on, but it has potential danger of safety; it isrepeled by immune system when it is injected to organism for a greatimmunogenicity. An injection with adenovirus vector with highconcentration, it leads to serious inflammatory reaction of liver. Viralvector such as liposome and polycation are commonly used lately. But,liposome and polycation have low specificness and targetness of genetransfer tissue, have lower transfection efficiency and short period ofgene expression, for they can be phagocytized by endothelial system.

许多的载体，病毒载体和非病毒载体以前已广泛应用，病毒载体主要包括腺病毒，逆转录病毒，腺相关病毒等；但病毒载体在安全性方面存在潜在的危险；免疫原性比较强，注射到机体后很快会被机体的免疫系统排斥，当静脉注射高浓度的腺病毒载体会使肝脏发生严重的炎症反应；非病毒载体目前常用的有脂质体及多聚阳离子聚合物；但脂质体和阳离子聚合物介导基因转移缺乏组织的特异性和靶向性，转染效率较低且易被网状内皮系统吞噬，基因表达时间短；因此研制新型的非病毒载体已成为研究的热点，纳米颗粒具有小尺寸效应，表面效应，随着颗粒直径变小，比表面积将会显著增大，故具有很高的化学活性，因而纳米成为了最有应用前景的非病毒载体。

At postnatal 1st week immunopositive reaction of NGF was detected mainly in sustentacular cells and the spermatogonia also showed positive staining. NGF positive staining in the testes was observed in interstitial cells, spermatogenetic cells, sustentacular cells and Leydig cells at 3rd week. After the postnatal 5th week, NGF-positive immunostaining was also detected in intersitial cells and spermatogenetic cells, but the intensity of reaction was weaker than that at 1st and 3rd weeks.

免疫组化定位分析显示：睾丸组织的神经生长因子蛋白表达于小鼠出生后的各个时期内，1周龄睾丸组织免疫阳性反应主要位于支持细胞，精原细胞也有着色；3周龄睾丸组织的间质细胞、各级生精细胞、支持细胞、管周肌样细胞表达均呈现阳性；5周后的睾丸组织内神经生长因子呈低水平表达，主要表达于间质细胞和生精细胞内。

To improve the stability of small unilamellar vesicles in vitro, the permeability of SUV from soybean phosphatidylcholine coated by collagen and chitosan was studied respectively by using 5(6)-Carboxyflourescein5(6-CF as a fluorescence probe.

为了提高小单层脂质体（SmalUnilamelarVesicles，SUV）的体外稳定性，以5（6）－羧基荧光素（5（6）－CF）为荧光探针，研究了脂质体被胶原蛋白和壳聚糖这两种天然生物大分子包覆后的通透性。

The recombinant plasmid DNA of PrM-E gene but not unmethylated CpG was capable of inducting antibodies against D2-43 virus.

我国登革2型病毒PrM-E基因重组质粒DNA具有一定的免疫原性。在本实验条件下，含非甲基化细菌性CpG的pUC19质粒DNA，对PrM-E基因的DNA免疫效果没有促进作用。

Results Incubation of TEC with MAC increased the expression of collagen type Ⅳ(P.05) in comparison with unstimulated cell.Conclusion MAC can i...

结论亚溶破剂量的MAC能使HKC的Ⅳ型胶原表达上调，可能是引起肾小管间质纤维化细胞外基质增多的原因之一。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。