原质

Objective: To examine the property and proliferation ability of primary culturing MSC, investgate the effect of lightly centrifugation and chemical definition medium to induce MSC differentiate into chondroblast.

目的：了解骨髓基质干细胞原代培养的特性及增殖能力，探讨轻度离心和化学限定培养基法诱导骨髓基质干细胞向成软骨方向分化的作用；为软骨组织工程提供足量、合适的种子细胞。

Now MSC become the chief seed-cell in cartilage tissue engineer, but the technology to stimulate MSC differentiate into chondroblast is not very successful,,the standard technology is culture the cell-ball in centrifuge tube,the reconstructured cartilage-like tissue is not achieve the mechanics of hyaline cartilage.

软骨组织工程的材料学研究主要体现在两类基质材料方面的进展，一类为天然基质材料，如胶原、壳聚糖、纤维素等；一类为人工合成可降解材料，如PGA、PLA等，但两者在来源、塑形能力及细胞识别信号等方面存在不足。

Abstract］ Objective To study the influence of different residual cornea bed of laser in situ keratomileusis on the collagenic construction of cornea through investigating the expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases in cornea cells for different residual stroma bed after LASIK for six months.

目的 研究不同切削厚度的准分子激光原位角膜磨镶术（laser in situ keratomileusis，LASIK）后兔眼基质金属蛋白酶（matrix metalloproteinases，MMPs）及基质金属蛋白酶组织抑制剂(tissue inhibitors of metalloproteinases，TIMPs)的表达，以探讨LASIK手术的不同切削深度对角膜的胶原结构的影响。

Following brain stem injury,the number of GFAP-positive astrocytes in cerebral cortex and pervent-ricular area,dorsomedial and ventro-medial pafts of midbrain at 30 min antemortem,and in periventricular area,dorsomedial part of pens,dorso-medial and ventro-medial part of medulla oblongata at 60 min antemortem.

采取针刺法造成实验大白鼠脑干损伤，用免疫组织化学LSAB法检测大脑、中脑、桥脑及延髓等不同部位脑组织中胶质原纤维酸性蛋白的改变。结果发现，生前损伤30min，大脑顶部灰质及中脑腹例中央部的GFAP阳性星形胶质细胞数目增多；生前损伤60min，除大脑顶部灰质及中脑腹侧中央部外。

CD34 hematopoietic stem/progenitor cells reside in the bone marrow in close proximity to the endosteal bone surface, surrounded by osteoblasts, stromal cells, and various extracellular matrix molecules. We used a bioartificial matrix of fibrillar collagen I, the major matrix component of bone, as a scaffold for ex vivo expansion of HSCs.

CD34 的造血干细胞/祖细胞存在于接近骨内膜表面的骨髓中，由成骨细胞、间充质细胞以及各种细胞外基质分子所包绕，我们采用生物人工合成的纤维胶原I

CD34+ hematopoietic stem/progenitor cells reside in the bone marrow in close proximity to the endosteal bone surface, surrounded by osteoblasts, stromal cells, and arious extracellular matrix molecules. We used a bioartificial matrix of fibrillar collagen I, the major matrix component of bone, as a scaffold for ex io expansion of HSCs.

CD34+的造血干细胞/祖细胞存在于接近骨内膜表面的骨髓中，由成骨细胞、间充质细胞以及各种细胞外基质分子所包绕，我们采用生物人工合成的纤维胶原I

Tooth is a typical epithelium-mesenchyme organ, which is formed through the mutual function between the original oral epithelium and epiblastic mesenchyme from the neural crest below.

牙齿是典型的上皮-间充质器官，由原口腔上皮和其下方的神经嵴来源的外胚间充质相互作用而形成。

Aim: To observe the effect of topiramate on the expression of glial fibrillary acidic protein in the hippocampus of rat kindled by pentylenetetrazole.

目的：观察托吡酯对戊四氮点燃大鼠海马胶质原纤维酸性蛋白表达的影响，以探讨胶质增生在癫发病机制中的作用。

Immunocytochemistry method was used to demonstrate the expression of glial fibrillary acidic protein.

用塑料吸头划刮培养皿，制造机械损伤的模型，继续培养至72 h，分别取出不同时间点的细胞进行胶质细胞增生情况的观察和免疫组织化学染色，对胶质原纤维酸性蛋白表达产物光密度进行图像分析。

Except for control group, rats in the other groups were intratracheally administered with bleomycin. Animals in pulmonary fibrosis model groups were sacrificed on day 3, 7, 14, 28 and 56. The sections of the right lung were stained by HE, Masson and sirius red. The left lung was weighed and its hydroxyproline content was assayed. The mRNAs of TGF-β1, MMP-9 and TIMP-1 in the lung homogenate were measured by semi-quantitative RT-PCR.

除N组外，其余各组采用气管内注入BLM致大鼠肺纤维化模型，分别于3、7、14、28、56d处死各组大鼠，右肺行苏木精－依红、Masson胶原及天狼猩红染色，测定左肺经脯氨酸的含量RT-PCR法半定量测定转化生长因子－1(TGF-β1)、基质金属蛋白酶－9(MMP-9)和基质金属蛋白酶组织抑制物－1(nMP-1)mRNA在肺内的表达。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。