原质

OBJECTIVE: To find the difference of gene sequence between human and porcine ECM and to identify the ECM immunogenicity based on bioinformatics.

目的：通过生物信息学的方法找到人与猪细胞外基质蛋白的基因序列差异，并制备抗体验证基质的免疫原性。

RESULTSdiabetic rats showed typical symptoms involving elevated serum glucose and weight loss (2)The rats in DM+VaD group reacted slower and made more error numbers in Y-maze than the rats in VaD group did, with a prolonged total reacting time in a whole day.(3)More neurons with serious damages, such as edema, ischemia or pyknosis could be found in DM+VaD group than in VaD group. More disarrangement of cone neurons, obvious glia proliferation, and more neuron apoptosis were found in CA area of hippocampus. CONCLUSIONS (1)2-VO in STZ-induced diabetic rats succeeded in establishing VaD animal models.(2) The cognitive dysfunction induced by VaD rats had been deteriorated by diabetes mellitus.(3)Morphology evidences proved that diabetes mellitus aggravated brain damages induced by VaD.PART II Effect of diabetes mellitus on the cholinergic nervous systemin CA1 area of hippocampus of VaDOBJECTIVE To estimate the role of cholinergic nervous system in hippocampus during the process of cognitive dysfunction aggravated by diabetes mellitus in DM+VaD group. METHODS Observe the changes of ChAT protein by immunohistochemistry stain. Chose three rats randomly from each group at each pointat two week, four week, and eight week, then sepa

经腹腔注射链脲佐菌素诱导慢性实验性糖尿病，1周后永久性结扎双侧颈总动脉(2-VO)制作VaD模型记录术后2周、4周和8周各组大鼠的体重及血糖；应用Y-迷宫检测空间定向学第二军医大学博士论文中…丈摘…要1ia；一色恤~﹁染澎糕行HE染色观察组织病理学孪叱；胶质细胞原纤维酸性蛋白protellZ，Cf冰尸标记观察星形胶质细舱的激活情况、橄声软公点娜育票票众糯黑橄居端粗默孺篇筑价井袱第二部分糖尿病对血管性痴呆大鼠海马c心区胆碱能神经系统的影响探讨海马CAI区胆碱能神经系统在糖尿病加重确D一大鼠认知障碍中所实验动物及分组同第一部分，应用免疫组织化学染色方法现察海马CAI{介狱牛第晕军l袭拔常博粗{论义

This study investigated the mechanisms that induce EMT and the significance of extracellular signal-regulated kinase cascade in this course in vitro.

本文旨在研究酸性成纤维细胞因子对上皮-间质转分化的影响，探讨有丝分裂原激动蛋白激酶的ERK信号传导途径在此过程中的作用，为肾小管-间质纤维化的防治提供理论依据。

Euchromatin was dominant and disthbuted evenly in cell nuclei, but surrounding cells and intercellular substance arranged disorderly, in lack of dense collagen fibers.

细胞核内以常染色质为主，且分布均匀，细胞周围及细胞间质结构稀疏、排列紊乱，缺乏紧密的胶原网络结构。

Antibody of mouse against rat of glial fibrilla acidic protein used to labeling astrocytes of the brain and antibody of rabbit against rat of c-fos labeling c-fos protein in the brain. The second fluorescence antibodies were FITC and Cy3, respectively. Observations of the results and quantitative analysis were conducted with LSCM.

在本实验中，我们应用免疫组织化学的双标方法，用小鼠抗大鼠胶质原纤维酸性蛋白标记大鼠脑内的星形胶质细胞，用兔抗大鼠c-fos标记大鼠脑内的c-fos蛋白，分别应用FITC和Cy3标记的荧光二抗来显示，最后用激光扫描共聚焦显微镜进行观察并定量分析。

Result: 1.(1) Three strips come to appear in the position of 6.0kb, 5.4kb and 600bp after gelose electrophoresis and their size accord with recombined plasmid DNA, pcDNA3 plasmid and VEGF165 gene accordingly.(2) Pure degree of the pcDNA3-VEGF165 recombined plasmid is: OD260/OD280=1.87. Its

在进行了重组质粒的扩增、提取、纯化、鉴定、兔MSCs的原代及传代培养以及RT-PCR、Western Blot 转染检测后，实验取得了令人满意的结果，证实了MSCs 能在体外有效转录外源VEGF 基因并分泌出VEGF 蛋白，同时也说明pcDNA3-VEGF165重组真核表达质粒是有效的。

Methods:Via a cannula iserted into adominal aorta, Gey's balanced salt solution containing 0.025% collagenase P was perfused into rat normal pancreas. After digestion with collagenase P and protease, Nycodenz density gradient centrifugation was performed to isolate PSC. PSC was identified by its morphology, cytoplasmic lipid droplets and immunocytochemical staining for desmin, GFAP and α-SMA. Results:The production,viability and purity of isolated PSC were（15.3 ± 4.6）× 103／g body weight,（95.0 ± 3.5）%, and ﹥80％ respectively.

大鼠腹主动脉插管灌注后，经胶原酶消化、Nycodenz密度梯度离心，分离得到PSC；通过观察细胞形态、胞质内脂滴和免疫细胞化学方法检测结蛋白、神经胶质酸纤维蛋白（glial fibrillary acidic protein，GFAP）、α平滑肌肌动素（α-smooth muscle actin，α-SMA）的表达来鉴定PSC。

On the 10th day, took out testis, fixed in the Boom fixtion solution, conventionally produced and section and stain. Finally observed under the optical microscope. Results The cytoplasm of mesenchymal cell and spermatogonium in test group showed hydropic degeneration.

结果 实验组小鼠睾丸组织中间质细胞和精原细胞胞质呈水样变性，出现红染细小的颗粒状物质，颗粒周围有大小不等的淡染区。

The associated minerals are topaz, lazulite, gorceixite, pyrophyllite, rutile etc. which constitute a suit of mineral assemblage of muddy or muddysandy muscovite schist in high pressure metamorphic belt.

与蓝晶石共生的黄玉、天蓝石、磷钙铝石、叶腊石、金红石等矿物构成高压变质带里原岩为泥质、泥砂质的白片岩或类白片岩的一套矿物组合。

For Goupitan Hydropwer Station on Wujiang River,over 1 200 anti-uplift anchor pile s are arranged in the water cushion pool to anchor the non-uniform soft rock strata composed of shale,clay rock,silty clay rock,thin layer of marlite and less medium-fine calcareous sandstones etc.

乌江构皮滩水电站水垫塘部位布置了1200多根抗浮锚桩，对页岩、粘土岩、粉砂质粘土岩，及夹薄层泥灰岩和少量中细粒钙质砂岩等不均质软岩层软岩进行锚固，原设计锚桩的间距为2.2m，为了优化设计，缩短工期，在施工前进行了4根锚桩原位抗拔试验，根据锚桩在抗拔过程中周边一定范围内岩体变形沿径向的分布规律，及破坏时裂隙的扩展形态和范围，确定了锚桩的间距，在此基础上提出了锚桩间距的优化方案，并应用于工程施工。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。